Abstract:
:The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt, and cadmium resistance in strain AE104, which is a cured derivative of the metal-resistant bacterium Alcaligenes eutrophus CH34. ncc is not expressed in Escherichia coli. The nre locus causes low-level nickel resistance in both Alcaligenes and E. coli strains. The nucleotide sequence of the ncc locus revealed seven open reading frames designated nccYXHCBAN. The corresponding predicted proteins share strong similarities with proteins encoded by the metal resistance loci cnr (cnrYXHCBA) and czc (czcRCBAD) of A. eutrophus CH34. When different DNA fragments carrying ncc genes were heterologously expressed under the control of the bacteriophage T7 promoter, five protein bands representing NccA (116 kDa), NccB (40 kDa), NccC (46 kDa), NccN (23.5 kDa), and NccX (16.5 kDa) were detected.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schmidt T,Schlegel HGdoi
10.1128/jb.176.22.7045-7054.1994subject
Has Abstractpub_date
1994-11-01 00:00:00pages
7045-54issue
22eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::A mutator mutation of Salmonella typhimurium, observed in a strain resistant to P22 and rifampin, affects mutation rates of bacterial, R factor, and prophage P22 genes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.638-639.1974
更新日期:1974-08-01 00:00:00
abstract::The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we used Affymetrix Antisense GeneChips to compare global gene expression profiles from isogenic MG1655 wild-type and Deltafnr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1135-1160.2005
更新日期:2005-02-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1298-1304.1966
更新日期:1966-03-01 00:00:00
abstract::Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6852-6855.2005
更新日期:2005-10-01 00:00:00
abstract::The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4664-4670.1997
更新日期:1997-08-01 00:00:00
abstract::A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1148-1152.1975
更新日期:1975-12-01 00:00:00
abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00834-08
更新日期:2008-12-01 00:00:00
abstract::Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.437-442.1983
更新日期:1983-04-01 00:00:00
abstract::An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.583-588.1977
更新日期:1977-08-01 00:00:00
abstract::The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01080-06
更新日期:2007-02-01 00:00:00
abstract::The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.8.2737-2746.2005
更新日期:2005-04-01 00:00:00
abstract::Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6358-6363.1993
更新日期:1993-10-01 00:00:00
abstract::One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2804-2812.1995
更新日期:1995-05-01 00:00:00
abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.159-168.1983
更新日期:1983-07-01 00:00:00
abstract::Species of marine bacteria belonging to the genus Beneckea and strains of Photobacterium fischeri were negatively stained and examined by means of the electron microscope to determine the structure and arrangement of their flagella. All of the species of the genus Beneckea had single, polar, sheathed flagella when gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.1.295-302.1971
更新日期:1971-07-01 00:00:00
abstract::The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6153-6159.1995
更新日期:1995-11-01 00:00:00
abstract::Das, S. K. (University of Calcutta, Calcutta, India) and G. C. Chatterjee. Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bacteriol. 83:1251-1259. 1962.-Staphylococcus aureus has been adapted to pyrithiamine, a thiamine analogue; as a result of this adaptation, the col...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1251-1259.1962
更新日期:1962-06-01 00:00:00
abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1090-1100.1974
更新日期:1974-06-01 00:00:00
abstract::The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5789-5794.1990
更新日期:1990-10-01 00:00:00
abstract::Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1044-1055.1969
更新日期:1969-06-01 00:00:00
abstract::Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.9.2312-2320.1998
更新日期:1998-05-01 00:00:00
abstract::The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.19.5460-5466.1995
更新日期:1995-10-01 00:00:00
abstract::Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.4.609-614.1961
更新日期:1961-10-01 00:00:00
abstract::Dehalogenases play key roles in the detoxification of halogenated aromatics. Interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme A (CoA) dehalogenase, has been reported. Here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01547-09
更新日期:2010-06-01 00:00:00
abstract::pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4809-4812.1995
更新日期:1995-08-01 00:00:00
abstract::Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00715-08
更新日期:2008-12-01 00:00:00
abstract::We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.1719-1724.1990
更新日期:1990-04-01 00:00:00
abstract::Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01445-10
更新日期:2011-03-01 00:00:00
abstract::Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1381-1388.2006
更新日期:2006-02-01 00:00:00