Topology analysis of the colicin V export protein CvaA in Escherichia coli.

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

journal_title：Journal of bacteriology

authors： Krantz MJ,Ballou CE

更新日期：1973-06-01 00:00:00

abstract：:CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...

journal_title：Journal of bacteriology

authors： Johnson VG,Nicholls PJ

更新日期：1994-08-01 00:00:00

abstract：:Streptococcus faecalis obtains metabolic energy chiefly from the conversion of glucose to lactic acid; the present experiments deal with the mechanism of lactic acid translocation across the cytoplasmic membrane. Efflux of [(14)C]lactate from preloaded cells was accelerated by raising the external pH, and also by the ...

journal_title：Journal of bacteriology

authors： Harold FM,Levin E

更新日期：1974-03-01 00:00:00

abstract：:Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...

journal_title：Journal of bacteriology

authors： Wong CY,Heuzenroeder MW,Quinn DM,Flower RL

更新日期：1997-06-01 00:00:00

abstract：:Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...

journal_title：Journal of bacteriology

authors： Golbeck JH,Albaugh SA,Radmer R

更新日期：1983-10-01 00:00:00

abstract：:The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322. These genes were mapped on the cloned DNA fragments by restriction endonuclease analysis and construction of the deletion derivatives. Their gene products were identified, by the minicell method, as proteins whose molecular weights w...

journal_title：Journal of bacteriology

authors： Homma M,Kutsukake K,Iino T

更新日期：1985-08-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...

journal_title：Journal of bacteriology

authors： Thiele I,Vo TD,Price ND,Palsson BØ

更新日期：2005-08-01 00:00:00

abstract：:Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

journal_title：Journal of bacteriology

authors： Bouillaut L,Self WT,Sonenshein AL

更新日期：2013-02-01 00:00:00

abstract：:Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...

journal_title：Journal of bacteriology

authors： Golub EE,Bronner F

更新日期：1974-09-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...

journal_title：Journal of bacteriology

authors： KOBAYASHI GS,FRIEDMAN L,KOFROTH JF

更新日期：1964-09-01 00:00:00

abstract：:We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

journal_title：Journal of bacteriology

authors： Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

更新日期：2011-04-01 00:00:00

abstract：:The mechanisms protecting nitrogenase in Gluconacetobacter diazotrophicus from damage by oxygen were studied. Evidence is provided suggesting that in G. diazotrophicus these mechanisms include respiratory protection as well as conformational protection in which a putative FeSII Shethna protein is involved. ...

journal_title：Journal of bacteriology

authors： Ureta A,Nordlund S

更新日期：2002-10-01 00:00:00

abstract：:We have identified a gene encoding a putative membrane protein homologous to the major facilitator superfamily, mapping upstream of the lacS gene in Sulfolobus solfataricus. Permeases from this family mediate secondary transport and are widely distributed among eubacteria and eukaryotes; the finding of an archaeal mem...

journal_title：Journal of bacteriology

authors： Prisco A,Moracci M,Rossi M,Ciaramella M

更新日期：1995-03-01 00:00:00

abstract：:Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...

journal_title：Journal of bacteriology

authors： Vaughan EE,van den Bogaard PT,Catzeddu P,Kuipers OP,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

journal_title：Journal of bacteriology

authors： ROGOSA M

更新日期：1964-01-01 00:00:00

abstract：:Selenium is a constituent in Escherichia coli of the anaerobic enzyme formate dehydrogenase in the form of selenocysteine. Selenium is also present in the tRNA of E. coli in the modified base 5-methylaminomethyl-2-selenouracil (mnm5Se2U). The pathways of bacterial selenium metabolism are largely uncharacterized, and i...

journal_title：Journal of bacteriology

authors： Kramer GF,Ames BN

更新日期：1988-02-01 00:00:00

abstract：:A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

journal_title：Journal of bacteriology

authors： Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

更新日期：2002-11-01 00:00:00

abstract：:The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

journal_title：Journal of bacteriology

authors： Reusch RN,Hiske TW,Sadoff HL

更新日期：1986-11-01 00:00:00

abstract：:The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced and was found to contain four open reading frames which were highly homologous to the traA, traL, traE, and traY gene pr...

journal_title：Journal of bacteriology

authors： Finlay BB,Frost LS,Paranchych W

更新日期：1986-11-01 00:00:00

abstract：:Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport p...

journal_title：Journal of bacteriology

authors： Sperandeo P,Lau FK,Carpentieri A,De Castro C,Molinaro A,Dehò G,Silhavy TJ,Polissi A

更新日期：2008-07-01 00:00:00

abstract：:The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

journal_title：Journal of bacteriology

authors： Zeilstra-Ryalls JH,Schornberg KL

更新日期：2006-01-01 00:00:00

abstract：:Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

journal_title：Journal of bacteriology

authors： Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

更新日期：2013-12-01 00:00:00

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein ...

journal_title：Journal of bacteriology

authors： Riegman N,Kusters R,Van Veggel H,Bergmans H,Van Bergen en Henegouwen P,Hacker J,Van Die I

更新日期：1990-02-01 00:00:00

abstract：:A chemically defined medium (MI(c)) is described in which cells of Haemophilus influenzae Rd grow rapidly (generation time, 35 +/- 5 min) and reach a stationary level of 10(10) cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that pu...

journal_title：Journal of bacteriology

authors： Herriott RM,Meyer EY,Vogt M,Modan M

更新日期：1970-02-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...

journal_title：Journal of bacteriology

authors： Komano T,Inouye S,Inouye M

更新日期：1980-12-01 00:00:00