Activation of the lac genes of Tn951 by insertion sequences from Pseudomonas cepacia.

Abstract:

:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to confer the ability to utilize lactose. The lac genes of Tn951 were poorly expressed in P. cepacia and were not induced by isopropyl-beta-D-thiogalactopyranoside. Lac+ variants of the pGC91.14-containing strains which formed beta-galactosidase at high constitutive levels as a consequence of transposition of insertion sequences from the P. cepacia genome to sites upstream of the lacZ gene of Tn951 were isolated. Certain of the elements also increased gene expression in other bacteria. For example, IS407 strongly activated the lacZ gene of Tn951 in Pseudomonas aeruginosa and Escherichia coli, and IS406 (but not IS407) did so in Zymomonas mobilis. The results indicate that IS elements from P. cepacia have potential for turning on the expression of foreign genes in a variety of gram-negative bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wood MS,Lory C,Lessie TG

doi

10.1128/jb.172.4.1719-1724.1990

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

1719-24

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

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