Abstract:
:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state). Sucrose-limited cultures exhibited linear relationships between qO2 and D, each of which, however, depended on the dissolved oxygen concentration in the range of 12 to 192 microM O2. From these plots, qO2 required for maintenance processes (mO2) were extrapolated. mO2 values did not increase linearly with increasing dissolved oxygen concentrations. With citrate- or acetate-limited cultures qO2 also depended on D. At 108 microM O2, however, qO2 and mO2 of the latter cultures were significantly lower than those of sucrose-limited cultures. Specific rates of acetylene reduction increased linearly with D, irrespective of the type of limitation and of the dissolved oxygen concentration (J. Kuhla and J. Oelze, Arch. Microbiol. 149:509-514, 1988). The reversible switch-off of nitrogenase activity under oxygen stress also depended on D and was independent of qO2, mO2, or the limiting substrate. Increased switch-off effects resulting from increased stress heights could be compensated for by increasing D. Since D represents not only the supply of the carbon source but also the supply of electrons and energy, the results suggest that the flux of electrons to the nitrogenase complex, rather than qO2, stabilizes nitrogenase activity against oxygen inactivation in aerobically growing A. vinelandii.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kuhla J,Oelze Jdoi
10.1128/jb.170.11.5325-5329.1988subject
Has Abstractpub_date
1988-11-01 00:00:00pages
5325-9issue
11eissn
0021-9193issn
1098-5530journal_volume
170pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.173.19.6132-6138.1991
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pub_type: 杂志文章
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更新日期:1970-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.119.2.469-477.1974
更新日期:1974-08-01 00:00:00
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更新日期:2001-12-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.177.12.3587-3588.1995
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.3979-3984.1995
更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4287-4290.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1429-1433.1983
更新日期:1983-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1962-06-01 00:00:00
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更新日期:1998-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.973-982.1977
更新日期:1977-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00