Sequence of the Streptococcus pneumoniae bacteriophage HB-3 amidase reveals high homology with the major host autolysin.

Abstract:

:We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This similarity completely disappeared outside the open reading frames coding for both amidases. The hbl gene transformed amidase-deficient strains of S. pneumoniae to the wild-type phenotype, and Southern blotting experiments provided evidence for recombination between donor and recipient genes. A comprehensive evaluation of these and previous results on the peptidoglycan hydrolases of S. pneumoniae and its bacteriophages suggested that recombination mechanisms participate in the evolution of the genes coding for these enzymes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Romero A,Lopez R,Garcia P

doi

10.1128/jb.172.9.5064-5070.1990

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

5064-70

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
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