Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.

Abstract:

:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyacrylamide gel electrophoresis. Molecular weights were estimated to be approximately 34,500 by several methods. Molecular sieve polyacrylamide gel electrophoresis indicated that the two esterases had the same molecular weight but different charge, which is indicative of isoenzymes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Shum AC,Markovetz AJ

doi

10.1128/JB.118.3.880-889.1974

subject

Has Abstract

pub_date

1974-06-01 00:00:00

pages

880-9

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

118

pub_type

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