Kinetics of uptake and incorporation of meso-diaminopimelic acid in different Escherichia coli strains.

abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

journal_title：Journal of bacteriology

authors： Srinivas S,Cronan JE

更新日期：2017-04-25 00:00:00

abstract：:Two open reading frames (ORFs) were identified immediately downstream of the four structural genes for the soluble hydrogenase (SH) of Alcaligenes eutrophus H16. While a mutation in ORF2 had no obvious effect on hydrogen metabolism, an in-frame deletion in ORF1, subsequently designated hoxW, led to a complete loss of ...

journal_title：Journal of bacteriology

authors： Thiemermann S,Dernedde J,Bernhard M,Schroeder W,Massanz C,Friedrich B

更新日期：1996-04-01 00:00:00

abstract：:Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

journal_title：Journal of bacteriology

authors： McCarthy D,Kupfer DM

更新日期：1987-02-01 00:00:00

abstract：:Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

journal_title：Journal of bacteriology

authors： Lubitz W,Harkness RE,Ishiguro EE

更新日期：1984-07-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00

abstract：:The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...

journal_title：Journal of bacteriology

authors： Wiater LA,Grindley ND

更新日期：1990-09-01 00:00:00

abstract：:Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produ...

journal_title：Journal of bacteriology

authors： Straight PD,Willey JM,Kolter R

更新日期：2006-07-01 00:00:00

abstract：:The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...

journal_title：Journal of bacteriology

authors： Kurn N,Shapiro L,Agabian N

更新日期：1977-09-01 00:00:00

abstract：:To assess the contributions of single-strand DNases (ssDNases) to recombination in a recBCD+ background, we studied 31 strains with all combinations of null alleles of exonuclease I (delta xon), exonuclease VII (xseA), RecJ DNase (recJ), and SbcCD DNase (sbcCD) and exonuclease I mutant alleles xonA2 and sbcB15. The xs...

journal_title：Journal of bacteriology

authors： Thoms B,Borchers I,Wackernagel W

更新日期：2008-01-01 00:00:00

abstract：:Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

journal_title：Journal of bacteriology

authors： Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

更新日期：2008-06-01 00:00:00

abstract：:The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

journal_title：Journal of bacteriology

authors： Aslanidis C,Schmitt R

更新日期：1990-04-01 00:00:00

abstract：:CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...

journal_title：Journal of bacteriology

authors： Shimkets LJ,Rafiee H

更新日期：1990-09-01 00:00:00

abstract：:The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

journal_title：Journal of bacteriology

authors： Bupp K,van Heijenoort J

更新日期：1993-03-01 00:00:00

abstract：:The histidine ATP-binding cassette (ABC) transporter of Salmonella enterica serovar Typhimurium is among the best-studied type I ABC import systems. The transporter consists of two transmembrane subunits, HisQ and HisM, and a homodimer of the nucleotide-binding subunit, HisP. Substrates are delivered by two periplasmi...

journal_title：Journal of bacteriology

authors： Heuveling J,Landmesser H,Schneider E

更新日期：2018-12-20 00:00:00

abstract：:Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...

journal_title：Journal of bacteriology

authors： Ogura M,Tanaka T

更新日期：1996-01-01 00:00:00

abstract：:Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...

journal_title：Journal of bacteriology

authors： Liu S,Yahr TL,Frank DW,Barbieri JT

更新日期：1997-03-01 00:00:00

abstract：:Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

journal_title：Journal of bacteriology

authors： KONDO E,MITSUHASHI S

更新日期：1964-11-01 00:00:00

abstract：:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

journal_title：Journal of bacteriology

authors： Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

更新日期：1998-10-01 00:00:00

abstract：:We present experimental evidence for the existence of multiple activator-binding sites in the upstream sequence of the ompC promoter, the expression of which is activated by the positive regulator OmpR in response to the osmolarity of the medium. We also found that a single OmpR-binding site can activate the ompC prom...

journal_title：Journal of bacteriology

authors： Maeda S,Mizuno T

更新日期：1990-01-01 00:00:00

abstract：:The PhoQ/PhoP two-component system is repressed by divalent cations, such as Mg(2+) and Ca(2+), in the growth medium and stimulated by low pH and certain cationic antimicrobial peptides. In Escherichia coli, it was recently shown that the histidine kinase PhoQ is also modulated by at least two additional factors, the ...

journal_title：Journal of bacteriology

authors： Lippa AM,Goulian M

更新日期：2012-03-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium modifies its lipopolysaccharide (LPS), including the lipid A portion, in response to changes in its environment including host tissues. The lipid A 3-O-deacylase PagL, the expression of which is promoted under a host-mimetic environment, exhibits latency in S. enterica; deacylat...

journal_title：Journal of bacteriology

authors： Manabe T,Kawasaki K

更新日期：2008-08-01 00:00:00

abstract：:Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...

journal_title：Journal of bacteriology

authors： Zacharski CA,Cooper TG

更新日期：1978-08-01 00:00:00

abstract：:Three species of Desulfovibrio were found to have a high degree of ribosomal ribonucleic acid homology with Desulfovibrio vulgaris. Desulfotomaculum nigrificans, which is also a sulfate-reducing anaerobe, had only 38% ribosomal ribonucleic acid homology with D. vulgaris. The homologies of six other unrelated genera we...

journal_title：Journal of bacteriology

authors： Pace B,Campbell LL

更新日期：1971-06-01 00:00:00

abstract：:Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

journal_title：Journal of bacteriology

authors： Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

更新日期：1995-08-01 00:00:00

abstract：:The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

journal_title：Journal of bacteriology

authors： Haddad JE,al-Jaufy AY,Jackson MP

更新日期：1993-08-01 00:00:00

abstract：:We designed a selection strategy for the isolation of Escherichia coli mutants exhibiting enhanced protein disulfide isomerase activity. The folding of a variant of tissue plasminogen activator (v-tPA), a protein containing nine disulfide bonds, in the bacterial periplasm is completely dependent on the level of disulf...

journal_title：Journal of bacteriology

authors： Zhan X,Gao J,Jain C,Cieslewicz MJ,Swartz JR,Georgiou G

更新日期：2004-02-01 00:00:00

abstract：:Immunological methods were used to examine the flagellin production of Salmonella typhimurium strains that carried a mutation in one of the two possible genes for flagellin (H1 or H2) and also were incapable of expressing the other gene. Some mutants produced flagellin that was excreted into the culture medium; others...

journal_title：Journal of bacteriology

authors： Homma M,Fujita H,Yamaguchi S,Iino T

更新日期：1987-01-01 00:00:00

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

journal_title：Journal of bacteriology

authors： White TC,Agabian N

更新日期：1995-09-01 00:00:00

abstract：:The concentrations of all metabolites studied, except fructose 1,6-bisphosphate from wild-type Phycomyces blakesleeanus, were light dependent. This photoregulation appears to be independent of the mad gene product(s) and also independent of carotene biosynthesis regulation. However, the photoregulation of glyceraldehy...

journal_title：Journal of bacteriology

authors： Rua J,Rodriguez-Aparicio LB,Busto F,Soler J

更新日期：1987-02-01 00:00:00