Role of surface protein SasG in biofilm formation by Staphylococcus aureus.

Abstract:

:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B domains, but not the A domain, are required. Purified recombinant B domain protein can form dimers in vitro in a Zn(2+)-dependent fashion. Furthermore, the protein can bind to cells that have B domains anchored to their surface and block biofilm formation. The full-length SasG protein exposed on the cell surface is processed within the B domains to a limited degree, resulting in cleaved proteins of various lengths being released into the supernatant. Some of the released molecules associate with the surface-exposed B domains that remain attached to the cell. Studies using inhibitors and mutants failed to identify any protease that could cause the observed cleavage within the B domains. Extensively purified recombinant B domain protein is very labile, and we propose that cleavage occurs spontaneously at labile peptide bonds and that this is necessary for biofilm formation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

doi

10.1128/JB.00628-10

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

5663-73

issue

21

eissn

0021-9193

issn

1098-5530

pii

JB.00628-10

journal_volume

192

pub_type

杂志文章
  • The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 activity, hrpG expression, and virulence.

    abstract::The rice pathogen recognition receptor, XA21, confers resistance to Xanthomonas oryzae pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. X. oryzae pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight rax (required for AvrXA21 activity) gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01406-07

    authors: Lee SW,Jeong KS,Han SW,Lee SE,Phee BK,Hahn TR,Ronald P

    更新日期:2008-03-01 00:00:00

  • Changes in DnaA-dependent gene expression contribute to the transcriptional and developmental response of Bacillus subtilis to manganese limitation in Luria-Bertani medium.

    abstract::The SOS response to DNA damage in bacteria is a well-known component of the complex transcriptional responses to genotoxic environmental stresses such as exposure to reactive oxygen species, alkylating agents, and many of the antibiotics targeting DNA replication. However, bacteria such as Bacillus subtilis also respo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00210-10

    authors: Hoover SE,Xu W,Xiao W,Burkholder WF

    更新日期:2010-08-01 00:00:00

  • The TolB protein interacts with the porins of Escherichia coli.

    abstract::TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7274-7279.1997

    authors: Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

    更新日期:1997-12-01 00:00:00

  • Induction of the copper resistance operon from Pseudomonas syringae.

    abstract::Cupric sulfate induced mRNA specific to the copper resistance gene cluster previously cloned from Pseudomonas syringae pv. tomato PT23. mRNA from each of the four genes of this cluster responded in a similar manner to induction over time and with different concentrations of cupric sulfate. Promoter fusion constructs i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4399-4401.1988

    authors: Mellano MA,Cooksey DA

    更新日期:1988-09-01 00:00:00

  • Catabolite-repression-like phenomenon in Rhizobium meliloti.

    abstract::We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.1197-1200.1978

    authors: Ucker DS,Signer ER

    更新日期:1978-12-01 00:00:00

  • Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates.

    abstract::Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7037-7043.2001

    authors: Kosugi A,Murashima K,Doi RH

    更新日期:2001-12-01 00:00:00

  • Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species.

    abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00519-09

    authors: Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

    更新日期:2009-09-01 00:00:00

  • Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.

    abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2540-2550.2005

    authors: Roy S,Ajitkumar P

    更新日期:2005-04-01 00:00:00

  • Pathway of succinate and propionate formation in Bacteroides fragilis.

    abstract::Cell suspensions of Bacteroides fragilis were allowed to ferment glucose and lactate labeled with (14)C in different positions. The fermentation products, propionate and acetate, were isolated, and the distribution of radioactivity was determined. An analysis of key enzymes of possible pathways was also made. The resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.84-91.1978

    authors: Macy JM,Ljungdahl LG,Gottschalk G

    更新日期:1978-04-01 00:00:00

  • Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

    abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.1776-1782.2003

    authors: Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

    更新日期:2003-03-01 00:00:00

  • Choline fermentation by Desulfovibrio desulfuricans.

    abstract::Baker, F. D. (Western Reserve University, Cleveland, Ohio), H. R. Papiska, and L. Leon Campbell. Choline fermentation by Desulfovibrio desulfuricans. J. Bacteriol. 84:973-978. 1962-Hayward and Stadtman pointed out that the organism they described as Vibrio cholinicus is closely related to Desulfovibrio desulfuricans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.5.973-978.1962

    authors: BAKER FD,PAPISKA HR,CAMPBELL LL

    更新日期:1962-11-01 00:00:00

  • Unusual C3 and C4 metabolism in the chemoautotroph Alcaligenes eutrophus.

    abstract::Phosphoenolpyruvate (PEP) carboxykinase was identified to be the only C3-carboxylating enzyme in Alcaligenes eutrophus. The enzyme requires GDP or inosine diphosphate (GTP or inosine triphosphate) for activity. Pyruvate- and other PEP-dependent CO2-fixing enzyme activities were not detected, regardless of whether the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.167-172.1984

    authors: Schobert P,Bowien B

    更新日期:1984-07-01 00:00:00

  • Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli.

    abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3359-3366.1988

    authors: Laspia MF,Wallace SS

    更新日期:1988-08-01 00:00:00

  • Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination.

    abstract::Alginates are polysaccharides composed of 1-4-linked beta-D-mannuronic acid and alpha-L-guluronic acid. The polymer can be degraded by alginate lyases, which cleave the polysaccharide using a beta-elimination reaction. Two such lyases have previously been identified in the soil bacterium Azotobacter vinelandii, as fol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00455-09

    authors: Gimmestad M,Ertesvåg H,Heggeset TM,Aarstad O,Svanem BI,Valla S

    更新日期:2009-08-01 00:00:00

  • Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

    abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5374-5381.1990

    authors: Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

    更新日期:1990-09-01 00:00:00

  • Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella tryphimurium.

    abstract::In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.227-233.1978

    authors: Lew HC,Nikaido H,Mäkelä PH

    更新日期:1978-10-01 00:00:00

  • Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.

    abstract::The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.664-670.1992

    authors: Huang L,Tsui P,Freundlich M

    更新日期:1992-02-01 00:00:00

  • Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.

    abstract::Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1554-1567.1992

    authors: Lam HM,Tancula E,Dempsey WB,Winkler ME

    更新日期:1992-03-01 00:00:00

  • Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain W23.

    abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.595-603.1971

    authors: Copeland JC

    更新日期:1971-02-01 00:00:00

  • Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.

    abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.6.1611-1616.1965

    authors: Ben-Hamida F,Schlessinger D

    更新日期:1965-12-01 00:00:00

  • Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein.

    abstract::The surface protein composition of members of a serogroup of Aeromonas hydrophila which exhibit high virulence for fish was examined. Treatment of whole cells of representative strain A. hydrophila TF7 with 0.2 M glycine buffer (pH 4.0) resulted in the release of sheets of a tetragonal surface protein array. Sodium do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.499-506.1988

    authors: Dooley JS,Trust TJ

    更新日期:1988-02-01 00:00:00

  • Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.

    abstract:UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00159-16

    authors: Maouche R,Burgos HL,My L,Viala JP,Gourse RL,Bouveret E

    更新日期:2016-06-13 00:00:00

  • Structural differences in the catalytic subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides.

    abstract::There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1188-1193.1985

    authors: Gibson JL,Tabita FR

    更新日期:1985-12-01 00:00:00

  • Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils.

    abstract::Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.4.919-923.1962

    authors: SHAPIRA J,LOWDEN L,HALE R

    更新日期:1962-04-01 00:00:00

  • Repair and antirepair DNA helicases in Helicobacter pylori.

    abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01848-07

    authors: Kang J,Blaser MJ

    更新日期:2008-06-01 00:00:00

  • The major facilitator superfamily-type transporter YmfE and the multidrug-efflux activator Mta mediate bacillibactin secretion in Bacillus subtilis.

    abstract::High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00464-08

    authors: Miethke M,Schmidt S,Marahiel MA

    更新日期:2008-08-01 00:00:00

  • Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

    abstract::A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

    更新日期:1982-10-01 00:00:00

  • Assembly site of bacteriophage f1 corresponds to adhesion zones between the inner and outer membranes of the host cell.

    abstract::Morphogenesis of the filamentous bacteriophage f1 occurred at adhesion zones between the inner and outer membranes of the host cell. Quantitation of adhesion zones in cells infected with mutant phage strains suggested that the phage gene I protein may be involved in the formation of adhesion zones for phage assembly. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.3.1270-1274.1985

    authors: Lopez J,Webster RE

    更新日期:1985-09-01 00:00:00

  • Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis.

    abstract::Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00747-09

    authors: Jude BA,Martinez RM,Skorupski K,Taylor RK

    更新日期:2009-11-01 00:00:00

  • Diguanylate cyclases control magnesium-dependent motility of Vibrio fischeri.

    abstract::Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00728-06

    authors: O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

    更新日期:2006-12-01 00:00:00