Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.

Abstract:

:Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures are diluted into medium containing 40 mug/ml of 5-fluorouracil (5-FU) but no inducer. However, if inducer (isopropylthiogalactoside) is left in the medium, enzyme formation in the presence of 5-FU continues for at least 11 min. Thus, inducer may increase the differential metabolic stability of the corresponding messenger ribonucleic acid (RNA; defined as the capacity to produce measurable enzyme) in inducible strains. However, such an interpretation requires that 5-FU rapidly arrest the further synthesis of messenger RNA competent to form active enzyme. C(14)-5-FU, like uracil, does appear to enter cells without measurable lag, saturating the pool of uracil nucleotides, and thereby the messenger RNA being formed, within several minutes. That 5-FU acts very quickly is also supported by the similar continuation of enzyme synthesis in the presence of inducer and antibiotics (actinomycin D and proflavine) which shut off all RNA synthesis, as well as by the response to 5-FU of enzyme synthesis in various constitutive mutants.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ben-Hamida F,Schlessinger D

doi

10.1128/JB.90.6.1611-1616.1965

subject

Has Abstract

pub_date

1965-12-01 00:00:00

pages

1611-6

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

90

pub_type

杂志文章
  • Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division.

    abstract::Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.989-997.1962

    authors: GRULA MM,GRULA EA

    更新日期:1962-05-01 00:00:00

  • Polypeptide involved in the Escherichia coli plasmid-mediated citrate transport system.

    abstract::A genetic determinant conferring on Escherichia coli the ability to utilize citrate as a sole source of carbon and energy was subcloned into pBR322 from a naturally occurring, citrate utilization (Cit+) plasmid, pOH30221, and was localized to a 1.6-kilobase region by cloning and subsequent deletion analysis. Genetic e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.421-426.1984

    authors: Hirato T,Shinagawa M,Ishiguro N,Sato G

    更新日期:1984-10-01 00:00:00

  • Protoplast transformation of glutamate-producing bacteria with plasmid DNA.

    abstract::A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.306-311.1984

    authors: Katsumata R,Ozaki A,Oka T,Furuya A

    更新日期:1984-07-01 00:00:00

  • Periplasmic structure of frozen-etched and negatively stained cells of Bacillus licheniformis as correlated with penicillinase formation.

    abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1002-1009.1969

    authors: Ghosh BK,Lampen JO,Remsen CC

    更新日期:1969-11-01 00:00:00

  • Cloning and characterization of the Haemophilus influenzae Rd rec-1+ gene.

    abstract::The Haemophilus influenzae Rd rec-1+ gene was cloned from a partial chromosomal digest into a plasmid vector as a 20-kilobase-pair (kbp) BstEII fragment and then subcloned. The smallest subclone with rec-1+ activity carried a 3.1-kbp EcoRI fragment. The identity of the rec-I gene in these clones was confirmed by trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4395-4401.1989

    authors: Stuy JH

    更新日期:1989-08-01 00:00:00

  • Nucleotide sequence and regulation of a gene involved in bile acid 7-dehydroxylation by Eubacterium sp. strain VPI 12708.

    abstract::Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2070-2077.1988

    authors: Coleman JP,White WB,Lijewski M,Hylemon PB

    更新日期:1988-05-01 00:00:00

  • The Importance of Being an Antagonist as Well as Persistent.

    abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...

    journal_title:Journal of bacteriology

    pub_type: 评论,杂志文章

    doi:10.1128/JB.00146-19

    authors: Progulske-Fox A

    更新日期:2019-05-08 00:00:00

  • Methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A and its repressibility by serine.

    abstract::The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1779-1789.1968

    authors: Albrecht AM,Pearce FK,Hutchison DJ

    更新日期:1968-05-01 00:00:00

  • Characterization of five genes in the upper-pathway operon of TOL plasmid pWW0 from Pseudomonas putida and identification of the gene products.

    abstract::The upper operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes which transform toluene and xylenes to benzoate and toluates. The genetic organization of the operon was characterized by cloning of the upper operon genes into an expression vector and identification of their products in Escherich...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.5048-5055.1989

    authors: Harayama S,Rekik M,Wubbolts M,Rose K,Leppik RA,Timmis KN

    更新日期:1989-09-01 00:00:00

  • ArcB1, a homolog of Escherichia coli ArcB, regulates dimethyl sulfoxide reduction in Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01695-09

    authors: Shroff NP,Charania MA,Saffarini DA

    更新日期:2010-06-01 00:00:00

  • Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon.

    abstract::5-methylcytosine in chromosomal DNA represents a potential source of frequent spontaneous mutation for hyperthermophiles. To determine the relevance of this threat for the archaeon Sulfolobus acidocaldarius, the mode of GGCC methylation by its restriction-modification system, SuaI, was investigated. Distinct isoschizo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.15.4657-4661.2003

    authors: Grogan DW

    更新日期:2003-08-01 00:00:00

  • Phenotypic selection and phase variation occur during alfalfa root colonization by Pseudomonas fluorescens F113.

    abstract::During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the ino...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.6.1587-1596.2002

    authors: Sánchez-Contreras M,Martín M,Villacieros M,O'Gara F,Bonilla I,Rivilla R

    更新日期:2002-03-01 00:00:00

  • Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae.

    abstract::Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.597-606.1979

    authors: Ausubel FM,Bird SC,Durbin KJ,Janssen KA,Margolskee RF,Peskin AP

    更新日期:1979-11-01 00:00:00

  • The LiaFSR system regulates the cell envelope stress response in Streptococcus mutans.

    abstract::Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01563-08

    authors: Suntharalingam P,Senadheera MD,Mair RW,Lévesque CM,Cvitkovitch DG

    更新日期:2009-05-01 00:00:00

  • Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.

    abstract::The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01956-07

    authors: Kazakov T,Vondenhoff GH,Datsenko KA,Novikova M,Metlitskaya A,Wanner BL,Severinov K

    更新日期:2008-04-01 00:00:00

  • Rsp inhibits attachment and biofilm formation by repressing fnbA in Staphylococcus aureus MW2.

    abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05454-11

    authors: Lei MG,Cue D,Roux CM,Dunman PM,Lee CY

    更新日期:2011-10-01 00:00:00

  • Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens.

    abstract::The dnaA genes of Salmonella typhimurium and Serratia marcescens, which complemented the temperature-sensitive dnaA46 mutation of Escherichia coli, were cloned and sequenced. They were very homologous to the dnaA gene of E. coli. The 63 N-terminal amino acids and the 333 C-terminal amino acids of the corresponding Dna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.3976-3981.1987

    authors: Skovgaard O,Hansen FG

    更新日期:1987-09-01 00:00:00

  • Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis.

    abstract::We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3104-3109.1987

    authors: Hahn J,Albano M,Dubnau D

    更新日期:1987-07-01 00:00:00

  • A novel cell surface polysaccharide in Pseudomonas putida WCS358, which shares characteristics with Escherichia coli K antigens, is not involved in root colonization.

    abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.7.1955-1961.1996

    authors: de Weger LA,Bloemberg GV,van Wezel T,van Raamsdonk M,Glandorf DC,van Vuurde J,Jann K,Lugtenberg BJ

    更新日期:1996-04-01 00:00:00

  • Mutations that improve export of maltose-binding protein in SecB- cells of Escherichia coli.

    abstract::It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4640-4647.1989

    authors: Collier DN,Bassford PJ Jr

    更新日期:1989-09-01 00:00:00

  • cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

    abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.7016-7019.1996

    authors: Tsang AW,Escalante-Semerena JC

    更新日期:1996-12-01 00:00:00

  • Nonrandom minichromosome replication in Escherichia coli K-12.

    abstract::The intervals between rounds of chromosome and minichromosome replication were measured by density shift experiments and found to be similar. Thus the minichromosome, a lambda asnA oriC bacteriophage, mostly replicates once each division cycle rather than randomly, despite its high copy number. Slight differences betw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.430-433.1987

    authors: Koppes LJ,von Meyenburg K

    更新日期:1987-01-01 00:00:00

  • The lantibiotic nisin induces transmembrane movement of a fluorescent phospholipid.

    abstract::Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid vesicles that contained a fluorescent phospholipid (1-acyl-2-(6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]caproyl)-sn-glycero-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.24.6565-6570.1998

    authors: Moll GN,Konings WN,Driessen AJ

    更新日期:1998-12-01 00:00:00

  • Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli.

    abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.353-357.1966

    authors: Fowler AV,Zabin I

    更新日期:1966-08-01 00:00:00

  • BamE modulates the Escherichia coli beta-barrel assembly machine component BamA.

    abstract::Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06426-11

    authors: Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

    更新日期:2012-03-01 00:00:00

  • Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma 32 in Escherichia coli.

    abstract::Accumulation of abnormal proteins in cells of bacteria or eukaryotes can induce synthesis of a set of heat shock proteins. We examined such induction following addition of azetidine (a proline analog) or synthesis of a heterologous protein (human prourokinase) in Escherichia coli. Synthesis of heat shock proteins unde...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.18.5648-5653.1994

    authors: Kanemori M,Mori H,Yura T

    更新日期:1994-09-01 00:00:00

  • Control of photosynthetic membrane assembly in Rhodobacter sphaeroides mediated by puhA and flanking sequences.

    abstract::A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.436-446.1989

    authors: Sockett RE,Donohue TJ,Varga AR,Kaplan S

    更新日期:1989-01-01 00:00:00

  • Heterogenetic antigens of gram-positive bacteria.

    abstract::Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.4.1440-1445.1966

    authors: Chorpenning FW,Dodd MC

    更新日期:1966-04-01 00:00:00

  • Nucleotide sequence of the tra YALE region from IncFV plasmid pED208.

    abstract::The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced and was found to contain four open reading frames which were highly homologous to the traA, traL, traE, and traY gene pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.990-998.1986

    authors: Finlay BB,Frost LS,Paranchych W

    更新日期:1986-11-01 00:00:00

  • Fluorescein amine media for rapid differentiation of staphylococci.

    abstract::Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.106-109.1961

    authors: KIMLER A

    更新日期:1961-07-01 00:00:00