Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon.

Abstract:

:5-methylcytosine in chromosomal DNA represents a potential source of frequent spontaneous mutation for hyperthermophiles. To determine the relevance of this threat for the archaeon Sulfolobus acidocaldarius, the mode of GGCC methylation by its restriction-modification system, SuaI, was investigated. Distinct isoschizomers of the SuaI endonuclease were used to probe the methylation state of GGCC in native S. acidocaldarius DNA. In addition, the methylation sensitivity of the SuaI endonuclease was determined with synthetic oligonucleotide substrates and modified natural DNAs. The results show that the SuaI system uses N(4) methylation to block cleavage of its recognition site, thereby avoiding the creation of G. T mismatches by spontaneous deamination at extremely high temperature.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Grogan DW

doi

10.1128/jb.185.15.4657-4661.2003

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

4657-61

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

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