Conservation of structure and location of Rhizobium meliloti and Klebsiella pneumoniae nifB genes.


:Using transposon Tn5-mediated mutagenesis, an essential Rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifA. Maxicell and DNA sequence analysis demonstrated that the new gene is transcribed in the same direction as nifA and codes for a 54-kilodalton protein. In Klebsiella pneumoniae, the nifBQ operon is located directly downstream of a gene which is structurally and functionally homologous to the R. meliloti nifA gene. The DNA sequences of the K. pneumoniae nifB and nifQ genes (which code for 51- and 20-kilodalton proteins, respectively) were determined. The DNA sequence of the newly identified R. meliloti gene was approximately 50% homologous to the K. pneumoniae nifB gene. R. meliloti does not contain a gene homologous to nifQ directly downstream of nifB. The R. meliloti nifB product shares approximately 40% amino acid homology with the K. pneumoniae nifB product, and 10 of the 12 cysteine residues of the R. meliloti nifB product are conserved with 10 of the 17 cysteine residues of the K. pneumoniae nifB product.


J Bacteriol


Journal of bacteriology


Buikema WJ,Klingensmith JA,Gibbons SL,Ausubel FM




Has Abstract


1987-03-01 00:00:00












  • Influence of genes encoding proton-translocating enzymes on suppression of Salmonella typhimurium growth and colonization.

    abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

    更新日期:1997-11-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • Threonine degradation by Serratia marcescens.

    abstract::The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Komatsubara S,Murata K,Kisumi M,Chibata I

    更新日期:1978-08-01 00:00:00

  • Genetic and structural organization of the aminophenol catabolic operon and its implication for evolutionary process.

    abstract::The aminophenol (AP) catabolic operon in Pseudomonas putida HS12 mineralizing nitrobenzene was found to contain all the enzymes responsible for the conversion of AP to pyruvate and acetyl coenzyme A via extradiol meta cleavage of 2-aminophenol. The sequence and functional analyses of the corresponding genes of the ope...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Park HS,Kim HS

    更新日期:2001-09-01 00:00:00

  • Role of the RepA1 protein in RepFIC plasmid replication.

    abstract::Using a sensitive primer extension technique, we have carried out studies to localize the start site of replication of the replicon RepFIC. In the course of these studies, we have found evidence that supports the hypothesis that transcription is an integral component of the initiation of replication. On the basis of o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maas R,Wang C

    更新日期:1997-04-01 00:00:00

  • Effects of homocitrate, homocitrate lactone, and fluorohomocitrate on nitrogenase in NifV- mutants of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Madden MS,Paustian TD,Ludden PW,Shah VK

    更新日期:1991-09-01 00:00:00

  • FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site.

    abstract::In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jyot J,Dasgupta N,Ramphal R

    更新日期:2002-10-01 00:00:00

  • Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures.

    abstract::The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chu H,Malone MM,Haldenwang WG,Walker JR

    更新日期:1977-10-01 00:00:00

  • Sulfolobus acidocaldarius synthesizes UMP via a standard de novo pathway: results of biochemical-genetic study.

    abstract::A genetic approach was used to establish the route of UMP biosynthesis in Sulfolobus acidocaldarius, a member of the hyperthermophilic division (the Crenarchaeota) of the Archaea domain. Pyrimidine auxotrophs of S. acidocaldarius DG6 were isolated by direct selection and by brute-force methods. Enzymatic assay of extr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Grogan DW,Gunsalus RP

    更新日期:1993-03-01 00:00:00

  • Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier.

    abstract::Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Engelke T,Jording D,Kapp D,Pühler A

    更新日期:1989-10-01 00:00:00

  • Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen.

    abstract::Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

    更新日期:2011-10-01 00:00:00

  • Mapping of a structural gene for valyl-transfer ribonucleic acid synthetase in Escherichia coli by transduction.

    abstract::A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tingle MA,Neidhardt FC

    更新日期:1969-05-01 00:00:00

  • An extracellular factor regulates expression of sdiA, a transcriptional activator of cell division genes in Escherichia coli.

    abstract::The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: García-Lara J,Shang LH,Rothfield LI

    更新日期:1996-05-01 00:00:00

  • Changes in sulfate transport characteristics and protein composition of Anacystis nidulans R2 during sulfur deprivation.

    abstract::Sulfur-starved cells of Anacystis nidulans have an increased capacity to take up sulfate. The apparent Vmax for sulfate uptake increased at least 10-fold after 24 h of sulfur deprivation, whereas the K1/2 remained unchanged at approximately 1.35 microM. The initial rate of sulfate uptake increased between 2 and 6 h af...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Green LS,Grossman AR

    更新日期:1988-02-01 00:00:00

  • Caulobacter flagellar organelle: synthesis, compartmentation, and assembly.

    abstract::In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lagenaur C,Agabian N

    更新日期:1978-09-01 00:00:00

  • Methylation-Induced Hypermutation in Natural Populations of Bacteria.

    abstract::Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cherry JL

    更新日期:2018-11-26 00:00:00

  • Occurrence of a class II restriction endonuclease in Staphylococcus aureus.

    abstract::The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stobberingh EE,Schiphof R,Sussenbach JS

    更新日期:1977-08-01 00:00:00

  • Citrate transport system of Streptococcus diacetilactis.

    abstract::Harvey, R. J. (University of California, Davis) and E. B. Collins. Citrate transport system of Streptococcus diacetilactis. J. Bacteriol. 83:1005-1009. 1962.-The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-05-01 00:00:00

  • Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.

    abstract::The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Reder A,Höper D,Gerth U,Hecker M

    更新日期:2012-07-01 00:00:00

  • The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20.

    abstract::Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rocco JM,Churchward G

    更新日期:2006-03-01 00:00:00

  • FtcR is a new master regulator of the flagellar system of Brucella melitensis 16M with homologs in Rhizobiaceae.

    abstract::The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome. These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Léonard S,Ferooz J,Haine V,Danese I,Fretin D,Tibor A,de Walque S,De Bolle X,Letesson JJ

    更新日期:2007-01-01 00:00:00

  • Polyamine transport and role of potE in response to osmotic stress in Escherichia coli.

    abstract::When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

    更新日期:2000-11-01 00:00:00

  • Homology of a plasmid from the spirochete Treponema denticola with the single-stranded DNA plasmids.

    abstract::The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: MacDougall J,Margarita D,Saint Girons I

    更新日期:1992-04-01 00:00:00

  • Developmental control of glucosamine and galactosamine levels during conidation in Neurospora crassa.

    abstract::The glucosamine and galactosamine content of mycelia was measured in cultures of Neurospora crassa grown on the surface of dialysis membranes. The glucosamine content was relatively constant throughout the different regions of the mycelial mat. The galactosamine content, however, was always lower in the growing-front ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schmit JC,Brody S

    更新日期:1975-06-01 00:00:00

  • Distribution and diversity of hsd genes in Escherichia coli and other enteric bacteria.

    abstract::We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Daniel AS,Fuller-Pace FV,Legge DM,Murray NE

    更新日期:1988-04-01 00:00:00

  • Control of cell growth in bacteria: experiments with thymine starvation.

    abstract::When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kubitschek HE

    更新日期:1971-02-01 00:00:00

  • Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination.

    abstract::The kinetic parameters of the release of Ca(2+)-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang P,Thomas S,Li YQ,Setlow P

    更新日期:2012-02-01 00:00:00

  • Ultraviolet sensitivity of Bacillus subtilis spores upon germination and outgrowth.

    abstract::A strain of Bacillus subtilis, UVSSP-42-1, which produces ultraviolet (UV)-sensitive spores and vegetative cells, was found to possess germinated spores 25 times more UV resistant than the resting spores. This relative resistance achieved upon germination was associated with the transition of the heat-resistant refrac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Munakata N

    更新日期:1974-10-01 00:00:00

  • Protein export by a gram-negative bacterium: production of aerolysin by Aeromonas hydrophila.

    abstract::The synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium Aeromonas hydrophila, was studied by pulse-labeling with [35S]methionine. The toxin was synthesized as a higher-molecular-weight precursor. This was processed cotranslationally, resulting in the appearance wit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Howard SP,Buckley JT

    更新日期:1985-03-01 00:00:00

  • Cytometric detection of mycobacterial surface antigens: exposure of mannosyl epitopes and of the arabinan segment of arabinomannans.

    abstract::The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ozanne V,Ortalo-Magne A,Vercellone A,Fournie JJ,Daffe M

    更新日期:1996-12-01 00:00:00