Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.

abstract：:Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

journal_title：Journal of bacteriology

authors： Dubourdieu M,DeMoss JA

更新日期：1992-02-01 00:00:00

abstract：:The Salmonella typhimurium LT2 ilvYC genes were studied by fusion of each gene to the Escherichia coli K-12 galK gene. The expression of ilvY and ilvC could then be determined by measurement of the galK-encoded galactokinase enzyme. The promoter for ilvC, pC, was located by this technique to a 0.42-kilobase BglII-EcoR...

journal_title：Journal of bacteriology

authors： Blazey DL,Burns RO

更新日期：1984-09-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:Lankford, C. E. (The University of Texas, Austin), James R. Walker, James B. Reeves, N. H. Nabbut, B. R. Byers, and R. J. Jones. Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). J. Bacteriol. 91:1070-1079. 1966.-When cells of Bacillus megaterium, grown o...

journal_title：Journal of bacteriology

authors： Lankford CE,Walker JR,Reeves JB,Nabbut NH,Byers BR,Jones RJ

更新日期：1966-03-01 00:00:00

abstract：:Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...

journal_title：Journal of bacteriology

authors： Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJ

更新日期：2009-10-01 00:00:00

abstract：:The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1979-12-01 00:00:00

abstract：:A gcr2 null mutant of Saccharomyces cerevisiae grows well on glucose in spite of its lower level of glycolytic enzymes between triose phosphates and pyruvate. A quantitative analysis shows that these levels are adequate to the flux but glycerate phosphates are elevated. ...

journal_title：Journal of bacteriology

authors： Uemura H,Fraenkel DG

更新日期：1999-08-01 00:00:00

abstract：:The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

journal_title：Journal of bacteriology

authors： Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

更新日期：1994-03-01 00:00:00

abstract：:The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...

journal_title：Journal of bacteriology

authors： Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

更新日期：2005-04-01 00:00:00

abstract：:Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

journal_title：Journal of bacteriology

authors： Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

更新日期：2000-12-01 00:00:00

abstract：:Injection of mice with a sublethal dose of endotoxin 2 hr after administration of 0.1 ml of carbon tetrachloride (CCl(4)) killed 75% of the animals. CCl(4) alone killed no controls. Significant protection against this effect was afforded by 5 mg of cortisone and by 5 mg of nicotinamide adenine dinucleotide (NAD). With...

journal_title：Journal of bacteriology

authors： Synder IS,Agarwal MK,Berry LJ

更新日期：1967-12-01 00:00:00

abstract：:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1998-12-01 00:00:00

abstract：:The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

journal_title：Journal of bacteriology

authors： Boone C,Sdicu A,Laroche M,Bussey H

更新日期：1991-11-01 00:00:00

abstract：:Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

journal_title：Journal of bacteriology

authors： ELLNER PD,BOHAN CD

更新日期：1962-02-01 00:00:00

abstract：:Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

journal_title：Journal of bacteriology

authors： Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

更新日期：1997-01-01 00:00:00

abstract：:There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

journal_title：Journal of bacteriology

authors： Nagarajan S,Sherman DM,Shaw I,Sherman LA

更新日期：2012-01-01 00:00:00

abstract：:Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

journal_title：Journal of bacteriology

authors： Lobanovska M,Tang CM,Exley RM

更新日期：2019-09-20 00:00:00

abstract：:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

journal_title：Journal of bacteriology

authors： Sanders DA,Mendez B,Koshland DE Jr

更新日期：1989-11-01 00:00:00

abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

journal_title：Journal of bacteriology

authors： Miki T,Shibagaki Y,Danbara H,Okada N

更新日期：2009-11-01 00:00:00

abstract：:Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol+, polA1, polC1026(ts), and polA1 polC1026(ts) cells after 137Cs gamma irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type II...

journal_title：Journal of bacteriology

authors： Hamelin C,Youngs DA,Smith KC

更新日期：1976-09-01 00:00:00

abstract：:A 3-hydroxypropionate/4-hydroxybutyrate cycle operates in autotrophic CO(2) fixation in various Crenarchaea, as studied in some detail in Metallosphaera sedula. This cycle and the autotrophic 3-hydroxypropionate cycle in Chloroflexus aurantiacus have in common the conversion of acetyl-coenzyme A (CoA) and two bicarbon...

journal_title：Journal of bacteriology

authors： Teufel R,Kung JW,Kockelkorn D,Alber BE,Fuchs G

更新日期：2009-07-01 00:00:00

abstract：:Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular c...

journal_title：Journal of bacteriology

authors： ENGER MD,SLEEPER BP

更新日期：1965-01-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome mark...

journal_title：Journal of bacteriology

authors： Bramucci MG,Lovett PS

更新日期：1977-09-01 00:00:00

abstract：:Altering membrane protein and lipid composition is an important strategy for maintaining membrane integrity during environmental stress. Many bacterial small RNAs (sRNAs) control membrane protein production, but sRNA-mediated regulation of membrane fatty acid composition is less well understood. The sRNA RydC was prev...

journal_title：Journal of bacteriology

authors： Bianco CM,Fröhlich KS,Vanderpool CK

更新日期：2019-09-06 00:00:00

abstract：:Sinorhizobium meliloti can live as a soil saprophyte and can engage in a nitrogen-fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma (σ) factors interacting wit...

journal_title：Journal of bacteriology

authors： Barnett MJ,Bittner AN,Toman CJ,Oke V,Long SR

更新日期：2012-09-01 00:00:00

abstract：:Incubation of ultraviolet-irradiated Escherichia coli B/r cultures with 0.7% Triton X-100 resulted in a large decrease in turbidity. Under phase-contrast optics, most of the irradiated detergent-treated cells were smaller than normal and of low phase density; only a small percentage were normal or larger than normal a...

journal_title：Journal of bacteriology

authors： Schenley RL,Fisher WD,Swenson PA,Khachatourians GG

更新日期：1976-05-01 00:00:00

abstract：:To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

journal_title：Journal of bacteriology

authors： Shaw GC,Sung CC,Liu CH,Kao HS

更新日期：1997-01-01 00:00:00

abstract：:Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

journal_title：Journal of bacteriology

authors： Choih SJ,Ferro AJ,Shapiro SK

更新日期：1977-07-01 00:00:00

abstract：:This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

journal_title：Journal of bacteriology

authors： Harley JB,Santangelo GM,Rasmussen H,Goldfine H

更新日期：1978-06-01 00:00:00