Abstract:
:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which shows structural similarity to the T3SS class V chaperones. An S. enterica serovar Typhimurium ssaE mutant failed to secrete SPI-2 translocator SseB and SPI-2-dependent effector PipB proteins. Coimmunoprecipitation and mass spectrometry analyses using an SsaE-FLAG fusion protein indicated that SsaE interacts with SseB and a putative T3SS-associated ATPase, SsaN. A series of deleted and point-mutated SsaE-FLAG fusion proteins revealed that the C-terminal coiled-coil domain of SsaE is critical for protein-protein interactions. Although SseA was reported to be a chaperone for SseB and to be required for its secretion and stability in the bacterial cytoplasm, an sseA deletion mutant was able to secrete the SseB in vitro when plasmid-derived SseB was overexpressed. In contrast, ssaE mutant strains could not transport SseB extracellularly under the same assay conditions. In addition, an ssaE(I55G) point-mutated strain that expresses the SsaE derivative lacking the ability to form a C-terminal coiled-coil structure showed attenuated virulence comparable to that of an SPI-2 T3SS null mutant, suggesting that the coiled-coil interaction of SsaE is absolutely essential for the functional SPI-2 T3SS and for Salmonella virulence. Based on these findings, we propose that SsaE recognizes translocator SseB and controls its secretion via SPI-2 type III secretion machinery.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Miki T,Shibagaki Y,Danbara H,Okada Ndoi
10.1128/JB.00863-09subject
Has Abstractpub_date
2009-11-01 00:00:00pages
6843-54issue
22eissn
0021-9193issn
1098-5530pii
JB.00863-09journal_volume
191pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.129.2.763-769.1977
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.176.11.3171-3176.1994
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更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.1.237-245.1978
更新日期:1978-04-01 00:00:00
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更新日期:1987-02-01 00:00:00
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更新日期:1991-10-01 00:00:00
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更新日期:2014-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00