Abstract:
:The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybrid E. coli strains with the nusASt substitution do not effectively support transcription antitermination mediated by the N gene product of phage lambda. We report the DNA sequence of nusASt, showing that the derived amino acid sequence is 95% identical to the derived amino acid sequence of nusAEc. The alignment of the amino acid sequences reveals scattered single amino acid differences and one region of significant heterogeneity. In this region, called 449, NusAEc has four amino acids and NusASt has nine amino acids. Functional studies of hybrid nusA genes, constructed from nusAEc and nusASt, show that the 449 region of the NusAEc protein is important for lambda N-mediated transcription antitermination. A hybrid that has a substitution of the four E. coli codons for the nine S. typhimurium codons, but is otherwise nusASt, supports the action of the N antitermination protein. The 449 region and, presumably, adjacent sequences appear to compose a functional domain of NusAEc important for the action of the N transcription antitermination protein of phage lambda.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DIdoi
10.1128/jb.176.5.1394-1404.1994subject
Has Abstractpub_date
1994-03-01 00:00:00pages
1394-404issue
5eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.822-824.1978
更新日期:1978-11-01 00:00:00
abstract::A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nit...
journal_title:Journal of bacteriology
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doi:10.1128/JB.163.1.385-388.1985
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doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
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abstract::The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.2045-2068.1966
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.318-320.1984
更新日期:1984-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.00933-10
更新日期:2011-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.470-474.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.176-182.1985
更新日期:1985-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2835-2841.1989
更新日期:1989-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2711-2715.1988
更新日期:1988-06-01 00:00:00
abstract::Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01445-10
更新日期:2011-03-01 00:00:00
abstract::CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3853-3856.1987
更新日期:1987-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.9.2602-2605.1995
更新日期:1995-05-01 00:00:00
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更新日期:2003-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.184.8.2281-2286.2002
更新日期:2002-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2292-2303.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.3979-3984.1995
更新日期:1995-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3810-3816.1988
更新日期:1988-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2427-2432.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.867-875.1968
更新日期:1968-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
abstract::The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.2.764-774.1975
更新日期:1975-11-01 00:00:00