Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato.


:A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subcloning PstI restriction endonuclease fragments of pCOP1 in the broad-host-range vector pRK404. A subclone containing a 4.4-kilobase PstI fragment conferred Cur on PS61. The Cur gene(s) was further located by insertional inactivation with Tn5. A subcloned fragment internal to the Cur determinant on pCOP2 was probed to plasmid and chromosomal DNA of four copper-resistant and three copper-sensitive strains of P. syringae pv. tomato. The probe hybridized to plasmids in resistant strains, but showed no detectable homology to copper-sensitive strains.


J Bacteriol


Journal of bacteriology


Bender CL,Cooksey DA




Has Abstract


1987-02-01 00:00:00












  • A DNA-binding peroxiredoxin of Coxiella burnetii is involved in countering oxidative stress during exponential-phase growth.

    abstract::Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hicks LD,Raghavan R,Battisti JM,Minnick MF

    更新日期:2010-04-01 00:00:00


    abstract::Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: HOWARD DH

    更新日期:1964-01-01 00:00:00


    abstract::Wiebers, Joyce L. (Purdue University, West Lafayette, Ind.), and Harold R. Garner. Use of S-methylcysteine and cystathionine by methionineless Neurospora mutants. J. Bacteriol. 88:1798-1804. 1964.-Radioactive methionine was found in hydrolysates of various strains of Neurospora crassa when either S-methylcysteine (SMC...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-12-01 00:00:00

  • Cell-bound cations of the moderately halophilic bacterium Vibrio costicola.

    abstract::Over most of the range of salt concentrations in which the moderately halophilic bacterium Vibrio costicola could grow, the sum of the cell-associated Na+ + K+ ions was at least as high as in the external medium. This is in contrast to other moderate halophiles, which have substantially lower internal than external sa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shindler DB,Wydro RM,Kushner DJ

    更新日期:1977-05-01 00:00:00

  • High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.

    abstract::The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fujita M,González-Pastor JE,Losick R

    更新日期:2005-02-01 00:00:00

  • Effect of iron on accumulation of exotoxin A-specific mRNA in Pseudomonas aeruginosa.

    abstract::A DNA probe from an internal fragment of the exotoxin A structural gene was used to study the effects of selected culture conditions on steady-state levels of exotoxin-specific mRNA in Pseudomonas aeruginosa. Cells grown under conditions of iron deprivation began to synthesize and excrete the exotoxin A polypeptide du...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lory S

    更新日期:1986-12-01 00:00:00

  • Diversity of genome structure in Salmonella enterica serovar Typhi populations.

    abstract::The genomes of most strains of Salmonella and Escherichia coli are highly conserved. In contrast, all 136 wild-type strains of Salmonella enterica serovar Typhi analyzed by partial digestion with I-CeuI (an endonuclease which cuts within the rrn operons) and pulsed-field gel electrophoresis and by PCR have rearrangeme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kothapalli S,Nair S,Alokam S,Pang T,Khakhria R,Woodward D,Johnson W,Stocker BA,Sanderson KE,Liu SL

    更新日期:2005-04-01 00:00:00

  • Transformation analysis of three linkage groups in Staphylococcus aureus.

    abstract::While studying a set of multiply marked mutants of Staphylococcus aureus strain 8325 by transformation, several instances of apparent genetic linkage were encountered. After showing that these linked transformations were readily inactivated by shearing of the deoxyribonucleic acid (DNA) but were resistant to dilution ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pattee PA,Neveln DS

    更新日期:1975-10-01 00:00:00

  • A subassembly of R27-encoded transfer proteins is dependent on TrhC nucleoside triphosphate-binding motifs for function but not formation.

    abstract::The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gilmour MW,Taylor DE

    更新日期:2004-03-01 00:00:00

  • Functional and structural analysis of HicA3-HicB3, a novel toxin-antitoxin system of Yersinia pestis.

    abstract::The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

    更新日期:2014-11-01 00:00:00

  • Transcriptome Analysis of Escherichia coli during dGTP Starvation.

    abstract:UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Itsko M,Schaaper RM

    更新日期:2016-05-13 00:00:00

  • Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase.

    abstract::Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

    更新日期:1992-03-01 00:00:00

  • Function of the sigma(E) regulon in dead-cell lysis in stationary-phase Escherichia coli.

    abstract::Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nitta T,Nagamitsu H,Murata M,Izu H,Yamada M

    更新日期:2000-09-01 00:00:00

  • Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification of a short motif required for action of the lambda N transcription antitermination protein.

    abstract::The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

    更新日期:1994-03-01 00:00:00

  • Manganese transport in Bacillus subtilis W23 during growth and sporulation.

    abstract::Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Eisenstadt E,Fisher S,Der CL,Silver S

    更新日期:1973-03-01 00:00:00

  • Participation of an extracellular deaminase in amino acid utilization by Neurospora crassa.

    abstract::A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: DeBusk RM,Ogilvie S

    更新日期:1984-08-01 00:00:00

  • Cosmid cloning of five Zymomonas trp genes by complementation of Escherichia coli and Pseudomonas putida trp mutants.

    abstract::A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Eddy CK,Smith OH,Noel KD

    更新日期:1988-07-01 00:00:00

  • trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon.

    abstract::Expression of the Bacillus subtilis trpEDCFBA operon has been shown to be regulated by transcription attenuation in response to the availability of L-tryptophan. Regulation is mediated by the tryptophan-activated trp RNA-binding attenuation protein, TRAP, the product of mtrB. Formation of mutually exclusive RNA anti-t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Merino E,Babitzke P,Yanofsky C

    更新日期:1995-11-01 00:00:00

  • Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: enhanced keratinase activity.

    abstract::Inorganic phosphate was found to decrease the caseinolytic and ethyl-esterase activities of the Microsporum gypseum germination protease. The germination protease possessed exokeratinase (beta-keratinase) activity immediately after release from the fungal spore. After phosphate treatment of the enzyme, the germination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Page WJ,Stock JJ

    更新日期:1974-02-01 00:00:00

  • Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.

    abstract::In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Auchtung JM,Lee CA,Grossman AD

    更新日期:2006-07-01 00:00:00

  • Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins.

    abstract::The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC. By inserting XbaI octamer linkers into the cloned ompB locus, four distinct frameshift mutants were isolated and subsequently characterize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Comeau DE,Ikenaka K,Tsung KL,Inouye M

    更新日期:1985-11-01 00:00:00

  • Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms.

    abstract::Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kawamoto I,Oka T,Nara T

    更新日期:1981-05-01 00:00:00

  • Phosphate acquisition components of the Myxococcus xanthus Pho regulon are regulated by both phosphate availability and development.

    abstract::In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Whitworth DE,Holmes AB,Irvine AG,Hodgson DA,Scanlan DJ

    更新日期:2008-03-01 00:00:00

  • Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142.

    abstract::Cyanothece sp. strain ATCC 51142 is a unicellular, diazotrophic cyanobacterium which demonstrated extensive metabolic periodicities of photosynthesis, respiration, and nitrogen fixation when grown under N2-fixing conditions. N2 fixation and respiration peaked at 24-h intervals early in the dark or subjective-dark peri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Colón-López MS,Sherman DM,Sherman LA

    更新日期:1997-07-01 00:00:00

  • High-quality draft genome sequence of the Opitutaceae bacterium strain TAV1, a symbiont of the wood-feeding termite Reticulitermes flavipes.

    abstract::Microbial communities in the termite hindgut are essential for degrading plant material. We present the high-quality draft genome sequence of the Opitutaceae bacterium strain TAV1, the first member of the phylum Verrucomicrobia to be isolated from wood-feeding termites. The genomic analysis reveals genes coding for li...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Isanapong J,Goodwin L,Bruce D,Chen A,Detter C,Han J,Han CS,Held B,Huntemann M,Ivanova N,Land ML,Mavromatis K,Nolan M,Pati A,Pennacchio L,Pitluck S,Szeto E,Tapia R,Woyke T,Rodrigues JL

    更新日期:2012-05-01 00:00:00

  • Amplification and deletion of the amyE+-tmrB+ gene region in a Bacillus subtilis recombinant-phage genome by the tmrA7 mutation.

    abstract::A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Furusato T,Takano J,Yamane K,Hashiguchi K,Tanimoto A,Mori M,Yoda K,Yamasaki M,Tamura G

    更新日期:1986-02-01 00:00:00

  • Iron is required to relieve inhibitory effects on NifL on transcriptional activation by NifA in Klebsiella pneumoniae.

    abstract::In Klebsiella pneumoniae, products of the nitrogen fixation nifLA operon regulate transcription of the other nif operons. NifA activates transcription by sigma54-holoenzyme. In vivo, NifL antagonizes the action of NifA under aerobic conditions or in the presence of combined nitrogen. In contrast to a previous report, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schmitz RA,He L,Kustu S

    更新日期:1996-08-01 00:00:00

  • Use of a phase variation-specific promoter of Myxococcus xanthus in a strategy for isolating a phase-locked mutant.

    abstract::The bacterium Myxococcus xanthus alternates between two colony types distinguished by colony morphology and pigmentation. Because the two phases are interconvertible, this phenomenon has been termed phase variation. In one phase, the colonies are bright yellow, rough, and swarming. In the alternate phase, the colonies...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Laue BE,Gill RE

    更新日期:1994-09-01 00:00:00

  • Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

    abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Verma A,Cheung AM,Burns DL

    更新日期:2008-11-01 00:00:00

  • Life cycle of Neurospora crassa viewed by scanning electron microscopy.

    abstract::Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Seale T

    更新日期:1973-02-01 00:00:00