Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato.


:A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subcloning PstI restriction endonuclease fragments of pCOP1 in the broad-host-range vector pRK404. A subclone containing a 4.4-kilobase PstI fragment conferred Cur on PS61. The Cur gene(s) was further located by insertional inactivation with Tn5. A subcloned fragment internal to the Cur determinant on pCOP2 was probed to plasmid and chromosomal DNA of four copper-resistant and three copper-sensitive strains of P. syringae pv. tomato. The probe hybridized to plasmids in resistant strains, but showed no detectable homology to copper-sensitive strains.


J Bacteriol


Journal of bacteriology


Bender CL,Cooksey DA




Has Abstract


1987-02-01 00:00:00












  • Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

    abstract::A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sancar GB

    更新日期:1985-02-01 00:00:00

  • Effects of a mutation that eliminates UDP glucose-pyrophosphorylase on the pathogenicity of Erwinia carotovora subsp. carotovora.

    abstract::A nonpathogenic mutant of Erwinia carotovora obtained by Mu d1 mutagenesis was defective in the ability to utilize several carbon sources. The basis of the mutation was analyzed biochemically and shown to be a defect in the ability to form UDP glucose-pyrophosphorylase. The nonpathogenic phenotype of the mutant was ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jayaswal RK,Bressan RA,Handa AK

    更新日期:1985-10-01 00:00:00

  • The mRNA for an inducible chloramphenicol acetyltransferase gene is cleaved into discrete fragments in Bacillus subtilis.

    abstract::cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ambulos NP Jr,Duvall EJ,Lovett PS

    更新日期:1987-03-01 00:00:00

  • Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus.

    abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brunskill EW,Bayles KW

    更新日期:1996-02-01 00:00:00

  • Colicin tolerance and map location of conjugation-deficient mutants.

    abstract::Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Davies JK,Reeves P

    更新日期:1975-07-01 00:00:00

  • Properties of Bacillus megaterium temperature-sensitive germination mutants.

    abstract::Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vary JC

    更新日期:1975-01-01 00:00:00

  • Trehalose metabolism by Bacillus popilliae.

    abstract::Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bhumiratana A,Anderson RL,Costilow RN

    更新日期:1974-08-01 00:00:00

  • Periplasmic structure of frozen-etched and negatively stained cells of Bacillus licheniformis as correlated with penicillinase formation.

    abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ghosh BK,Lampen JO,Remsen CC

    更新日期:1969-11-01 00:00:00

  • Determinants for the subcellular localization and function of a nonessential SEDS protein.

    abstract::The Bacillus subtilis SpoVE integral membrane protein is essential for the heat resistance of spores, probably because of its involvement in spore peptidoglycan synthesis. We found that an SpoVE-yellow fluorescent protein (YFP) fusion protein becomes localized to the forespore during the earliest stages of engulfment,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Real G,Fay A,Eldar A,Pinto SM,Henriques AO,Dworkin J

    更新日期:2008-01-01 00:00:00

  • AerR, a second aerobic repressor of photosynthesis gene expression in Rhodobacter capsulatus.

    abstract::Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dong C,Elsen S,Swem LR,Bauer CE

    更新日期:2002-05-01 00:00:00

  • Rapid transient growth at low pH in the cyanobacterium Synechococcus sp.

    abstract::The thermophilic cyanobacterium Synechococcus sp. strain Y-7c-s grows at its maximum rate at a high pH (pH 8 and above) the does not show sustained growth below pH 6.5. However, rapidly growing, exponential-phase cells from high-pH cultures continued to grow rapidly for several hours after transfer to pH 6.0 or 5.0. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kallas T,Castenholz RW

    更新日期:1982-01-01 00:00:00

  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

    abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00

  • Regulation of the SOS response analyzed by RecA protein amplification.

    abstract::A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Calsou P,Salles B

    更新日期:1985-06-01 00:00:00

  • Characterization of Saccharomycopsis lipolytica mutants that express temperature-sensitive synthesis of isocitrate lyase.

    abstract::Four mutants specifically deficient in the activity of isocitrate lyase were independently isolated in the alkane yeast Saccharomycopsis lipolytica. Genetic analysis by means of protoplast fusion and mitotic haploidization revealed that the mutations were recessive and non-complementary at a single genetic locus, icl....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Matsuoka M,Himeno T,Aiba S

    更新日期:1984-03-01 00:00:00

  • Accumulation of sulfite by a sulfate-using revertant of Salmonella pullorum.

    abstract::Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kline BC,Schoenhard DE

    更新日期:1969-10-01 00:00:00

  • Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii.

    abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dowhanick TM,Russell I,Scherer SW,Stewart GG,Seligy VL

    更新日期:1990-05-01 00:00:00

  • Extracellular secretion of noncatalytic plant cell wall-binding proteins by the cellulolytic thermophile Caldicellulosiruptor bescii.

    abstract::Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yokoyama H,Yamashita T,Morioka R,Ohmori H

    更新日期:2014-11-01 00:00:00

  • Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast.

    abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lorenz RT,Parks LW

    更新日期:1987-08-01 00:00:00

  • Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.

    abstract::The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Huang L,Tsui P,Freundlich M

    更新日期:1992-02-01 00:00:00

  • Enzymatic and non-enzymatic reduction of nitrite by extracts of Neurospora crassa.

    abstract::Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

    更新日期:1975-08-01 00:00:00

  • hisT is part of a multigene operon in Escherichia coli K-12.

    abstract::The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

    更新日期:1985-01-01 00:00:00

  • Structure and organization of the pel genes from Erwinia chrysanthemi EC16.

    abstract::The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

    更新日期:1988-08-01 00:00:00

  • Convenient transduction of recA with bacteriophage T4GT7.

    abstract::The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Plakidou S,Moffat KG,Salmond GP,Mackinnon G

    更新日期:1984-09-01 00:00:00

  • Complete genome sequence of Burkholderia sp. Strain GG4, a betaproteobacterium that reduces 3-oxo-N-acylhomoserine lactones and produces different N-acylhomoserine lactones.

    abstract::Burkholderia sp. strain GG4, isolated from the ginger rhizosphere, possesses a unique N-acylhomoserine lactone (AHL)-modifying activity that reduces 3-oxo-AHLs to 3-hydroxy-AHLs. To the best of our knowledge, this is the first sequenced genome from a bacterium of the genus Burkholderia that shows both quorum-sensing a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hong KW,Koh CL,Sam CK,Yin WF,Chan KG

    更新日期:2012-11-01 00:00:00

  • The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.

    abstract::Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: O'Donnell SM,Janssen GR

    更新日期:2001-02-01 00:00:00

  • Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

    abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rich JJ,Willis DK

    更新日期:1997-04-01 00:00:00

  • PapD, a periplasmic transport protein in P-pilus biogenesis.

    abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lindberg F,Tennent JM,Hultgren SJ,Lund B,Normark S

    更新日期:1989-11-01 00:00:00

  • The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit.

    abstract::It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sikora AE,Zielke R,Datta K,Maddock JR

    更新日期:2006-02-01 00:00:00


    abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: WEAVER JR,PATTEE PA

    更新日期:1964-09-01 00:00:00

  • Mechanism of action of the antifungal antibiotic pyrrolnitrin.

    abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tripathi RK,Gottlieb D

    更新日期:1969-10-01 00:00:00