Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato.

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...

journal_title：Journal of bacteriology

authors： Noda S,Takezawa Y,Mizutani T,Asakura T,Nishiumi E,Onoe K,Wada M,Tomita F,Matsushita K,Yokota A

更新日期：2006-10-01 00:00:00

abstract：:Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...

journal_title：Journal of bacteriology

authors： Robertson J,Smook E

更新日期：1976-11-01 00:00:00

abstract：:Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...

journal_title：Journal of bacteriology

authors： Pfister A,DeVries JK,Maas WK

更新日期：1976-07-01 00:00:00

abstract：:The Amt proteins constitute a ubiquitous family of transmembrane ammonia channels that permit the net uptake of ammonium by cells. In many organisms, there is more than one amt gene, and these genes are subjected to nitrogen control. The mature Amt protein is a homo- or heterooligomer of three Amt subunits. We previou...

journal_title：Journal of bacteriology

authors： Paz-Yepes J,Herrero A,Flores E

更新日期：2007-11-01 00:00:00

abstract：:A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinat...

journal_title：Journal of bacteriology

authors： Edwards DL,Belsole DM,Guzik HJ,Unger BW

更新日期：1979-02-01 00:00:00

abstract：:The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

journal_title：Journal of bacteriology

authors： Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

更新日期：2003-10-01 00:00:00

abstract：:Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

journal_title：Journal of bacteriology

authors： Okanishi M,Gregory KF

更新日期：1970-12-01 00:00:00

abstract：:The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...

journal_title：Journal of bacteriology

authors： Gao T,O'Brian MR

更新日期：2005-08-01 00:00:00

abstract：UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...

journal_title：Journal of bacteriology

authors： Lin TY,Santos TM,Kontur WS,Donohue TJ,Weibel DB

更新日期：2015-11-01 00:00:00

abstract：:The polysaccharide intercellular adhesin or the cell wall-anchored accumulation-associated protein (Aap) mediates cellular accumulation during Staphylococcus epidermidis biofilm maturation. Mutation of sortase, which anchors up to 11 proteins (including Aap) to the cell wall, blocked biofilm development by the cerebro...

journal_title：Journal of bacteriology

authors： Conlon BP,Geoghegan JA,Waters EM,McCarthy H,Rowe SE,Davies JR,Schaeffer CR,Foster TJ,Fey PD,O'Gara JP

更新日期：2014-12-01 00:00:00

abstract：:An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity...

journal_title：Journal of bacteriology

authors： Zulty JJ,Speedie MK

更新日期：1989-12-01 00:00:00

abstract：:After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

journal_title：Journal of bacteriology

authors： Sargent MG,Bennett MF

更新日期：1986-04-01 00:00:00

abstract：:Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contrib...

journal_title：Journal of bacteriology

authors： Hassett DJ

更新日期：1996-12-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

journal_title：Journal of bacteriology

authors： Hennig S,Ziebuhr W

更新日期：2008-02-01 00:00:00

abstract：:l-Lysine caused repression of diaminopimelate decarboxylase synthesis in Bacillus cereus when grown in either a minimal defined medium (CDGS medium) or a complex defined medium (a modified lysine assay medium). When cells were grown in either of the two media, variations in the specific activity of the enzyme as a fun...

journal_title：Journal of bacteriology

authors： Grandgenett DP,Stahly DP

更新日期：1971-05-01 00:00:00

abstract：:Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...

journal_title：Journal of bacteriology

authors： Lindstedt G,Lindstedt S,Midtvedt T,Tofft M

更新日期：1970-03-01 00:00:00

abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

journal_title：Journal of bacteriology

authors： Vela GR,Cagle GD,Holmgren PR

更新日期：1970-11-01 00:00:00

abstract：:A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...

journal_title：Journal of bacteriology

authors： Chaudhry GR,Huang GH

更新日期：1988-09-01 00:00:00

abstract：:We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

journal_title：Journal of bacteriology

authors： Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

更新日期：2007-03-01 00:00:00

abstract：:The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein...

journal_title：Journal of bacteriology

authors： Dickinson DP,Kubiniec MA,Yoshimura F,Genco RJ

更新日期：1988-04-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

journal_title：Journal of bacteriology

authors： Leventhal JM,Chambliss GH

更新日期：1982-12-01 00:00:00

abstract：:Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resu...

journal_title：Journal of bacteriology

authors： Cook TM,Deitz WH,Goss WA

更新日期：1966-02-01 00:00:00

abstract：:Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...

journal_title：Journal of bacteriology

authors： FALCONE G,NICKERSON WJ

更新日期：1963-04-01 00:00:00

abstract：:An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...

journal_title：Journal of bacteriology

authors： Makula RA,Finnerty WR

更新日期：1971-09-01 00:00:00

abstract：:The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

journal_title：Journal of bacteriology

authors： Stitt BL,Mosig G

更新日期：1989-07-01 00:00:00

abstract：:We report the cloning and characterization of the biosynthetic gene cluster (crtE, crtB, crtI, crtE2, crtYg, crtYh, and crtX) of the γ-cyclic C(50) carotenoid sarcinaxanthin in Micrococcus luteus NCTC2665. Expression of the complete and partial gene cluster in Escherichia coli hosts revealed that sarcinaxanthin biosyn...

journal_title：Journal of bacteriology

authors： Netzer R,Stafsnes MH,Andreassen T,Goksøyr A,Bruheim P,Brautaset T

更新日期：2010-11-01 00:00:00

abstract：:The terminal step of triacylglycerol (TAG) formation in the yeast Saccharomyces cerevisiae is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase (DAGAT). In this study we demonstrate that the gene product of YOR245c, Dga1p, catalyzes a major yeast DAGAT activity which is localized to lipid particles. Enzy...

journal_title：Journal of bacteriology

authors： Sorger D,Daum G

更新日期：2002-01-01 00:00:00