A simplified methylcoenzyme M methylreductase assay with artificial electron donors and different preparations of component C from Methanobacterium thermoautotrophicum delta H.

abstract：:Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...

journal_title：Journal of bacteriology

authors： Veluthambi K,Krishnan M,Gould JH,Smith RH,Gelvin SB

更新日期：1989-07-01 00:00:00

abstract：:Inorganic phosphate was found to decrease the caseinolytic and ethyl-esterase activities of the Microsporum gypseum germination protease. The germination protease possessed exokeratinase (beta-keratinase) activity immediately after release from the fungal spore. After phosphate treatment of the enzyme, the germination...

journal_title：Journal of bacteriology

authors： Page WJ,Stock JJ

更新日期：1974-02-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which ant...

journal_title：Journal of bacteriology

authors： Abdou L,Chou HT,Haas D,Lu CD

更新日期：2011-06-01 00:00:00

abstract：:The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...

journal_title：Journal of bacteriology

authors： Every D,Skerman TM

更新日期：1980-02-01 00:00:00

abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

journal_title：Journal of bacteriology

authors： Miki T,Shibagaki Y,Danbara H,Okada N

更新日期：2009-11-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：:Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...

journal_title：Journal of bacteriology

authors： Kilstrup M,Meng LM,Neuhard J,Nygaard P

更新日期：1989-04-01 00:00:00

abstract：:Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

journal_title：Journal of bacteriology

authors： Begg KJ,Donachie WD

更新日期：1985-08-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

journal_title：Journal of bacteriology

authors： Koide T,Vêncio RZ,Gomes SL

更新日期：2006-08-01 00:00:00

abstract：:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

journal_title：Journal of bacteriology

authors： Consevage MW,Phillips AT

更新日期：1990-05-01 00:00:00

abstract：:The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

journal_title：Journal of bacteriology

authors： David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

更新日期：1990-10-01 00:00:00

abstract：:A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

journal_title：Journal of bacteriology

authors： Lee JH,Wendt JC,Shanmugam KT

更新日期：1990-04-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...

journal_title：Journal of bacteriology

authors： Janjusevic R,Quezada CM,Small J,Stebbins CE

更新日期：2013-02-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

journal_title：Journal of bacteriology

authors： Beeser AE,Cooper TG

更新日期：1999-09-01 00:00:00

abstract：:A procedure for efficient transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts with plasmid DNA was developed. Transformation frequencies with S. fradiae protoplasts were strongly influenced by the temperatures for cell growth, protoplast formation, and protoplast regeneration. Transformatio...

journal_title：Journal of bacteriology

authors： Matsushima P,Baltz RH

更新日期：1985-07-01 00:00:00

abstract：:A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...

journal_title：Journal of bacteriology

authors： Suwanto A,Kaplan S

更新日期：1989-11-01 00:00:00

abstract：:Ragland, T. E. (Brandeis University, Waltham, Mass.), T. Kawasaki, and J. M. Lowenstein. Comparative aspects of some bacterial dehydrogenases and transhydrogenases. J. Bacteriol. 91:236-244. 1966.-Twenty-eight diverse bacterial species were surveyed for the activities and coenzyme specificities of four enzymes: isocit...

journal_title：Journal of bacteriology

authors： Ragland TE,Kawasaki T,Lowenstein JM

更新日期：1966-01-01 00:00:00

abstract：:A primary alcohol dehydrogenase has been purified from Methylococcus capsulatus (Texas strain). The purified enzyme catalyzes the oxidation of methanol and formaldehyde to formate; other primary alcohols are oxidized to their corresponding aldehydes. Ammonium ions are required for enzyme activity. The enzyme has a mol...

journal_title：Journal of bacteriology

authors： Patel RN,Bose HR,Mandy WJ,Hoare DS

更新日期：1972-05-01 00:00:00

abstract：:Bacteroides ovatus, a human colonic anaerobe, utilizes the galactomannan guar gum as a sole source of carbohydrate. Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragm...

journal_title：Journal of bacteriology

authors： Gherardini FC,Salyers AA

更新日期：1987-05-01 00:00:00

abstract：:Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...

journal_title：Journal of bacteriology

authors： Yang F,Curran SC,Li LS,Avarbock D,Graf JD,Chua MM,Lu G,Salem J,Rubin H

更新日期：1997-10-01 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...

journal_title：Journal of bacteriology

authors： Friedberg F,Rhodes C

更新日期：1986-03-01 00:00:00

abstract：:Exponentially growing cells of the gram-negative bacterium Chromobacterium violaceum demonstrate invaginations of the cytoplasmic membrane with a high frequency. These invaginations conform to the ultrastructural appearance of mesosomes of gram-positive bacteria. As many as four mesosomes are observed per cell, each o...

journal_title：Journal of bacteriology

authors： Rucinsky TE,Cota-Robles EH

更新日期：1974-05-01 00:00:00

abstract：:Genome sequences from the prolific Roseobacter clade in the Alphaproteobacteria are beginning to reveal the genetic basis for the diverse lifestyles of these organisms. Here we present the genome sequences of Oceanicola granulosus HTCC2516(T) and Oceanicola batsensis HTCC2597(T), two marine Roseobacter species isolate...

journal_title：Journal of bacteriology

authors： Thrash JC,Cho JC,Vergin KL,Giovannoni SJ

更新日期：2010-07-01 00:00:00

abstract：:The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...

journal_title：Journal of bacteriology

authors： Chouayekh H,Nothaft H,Delaunay S,Linder M,Payrastre B,Seghezzi N,Titgemeyer F,Virolle MJ

更新日期：2007-02-01 00:00:00

abstract：:As part of an analysis of the conjugative transfer genes associated with the expression of F pili by plasmid F, we have investigated the physical location of the traC and traW genes. We found that plasmid clones carrying a 2.95-kilobase EcoRI-EcoRV F transfer operon fragment were able to complement transfer of F lac t...

journal_title：Journal of bacteriology

authors： Maneewannakul S,Kathir P,Moore D,Le LA,Wu JH,Ippen-Ihler K

更新日期：1987-11-01 00:00:00