Abstract:
:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total of 184 sites were polymorphic, and up to 6% sequence divergence was observed between pairs of strains. The deduced amino acid sequences showed much more variation than had been shown by multilocus enzyme electrophoresis, and in addition the net charge calculated did not correlate strongly with electrophoretic mobility. A phylogenetic tree for the sequences that was based on maximum parsimony differed significantly from a tree for the same strains that was based on multilocus enzyme electrophoresis for 35 enzymes (R. K. Selander, D. A. Caugant, and T. S. Whittam, p. 1625-1648, in F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger, ed., Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 1987). These data, together with analysis of sequence variation between the strains, indicated that intragenic recombination and transfer of the whole of gnd have occurred in the evolution of these strains. There is evidence of one recombination event between E. coli and Salmonella typhimurium.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bisercić M,Feutrier JY,Reeves PRdoi
10.1128/jb.173.12.3894-3900.1991subject
Has Abstractpub_date
1991-06-01 00:00:00pages
3894-900issue
12eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5611-5614.2000
更新日期:2000-10-01 00:00:00
abstract::When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.744-746.1977
更新日期:1977-11-01 00:00:00
abstract::Preliminary evidence has been presented by Guilfoile and Hutchinson (J. Bacteriol. 174:3651-3658, 1992) suggesting that the Streptomyces glaucescens TcmR protein is a transcriptional repressor. Here, we extend that work by showing that transcription of the S. glaucescens tcmA gene is inducible by tetracenomycin C and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3659-3666.1992
更新日期:1992-06-01 00:00:00
abstract::The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5448-5457.1997
更新日期:1997-09-01 00:00:00
abstract::A genetic determinant conferring on Escherichia coli the ability to utilize citrate as a sole source of carbon and energy was subcloned into pBR322 from a naturally occurring, citrate utilization (Cit+) plasmid, pOH30221, and was localized to a 1.6-kilobase region by cloning and subsequent deletion analysis. Genetic e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.421-426.1984
更新日期:1984-10-01 00:00:00
abstract::During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5384-5388.1999
更新日期:1999-09-01 00:00:00
abstract::Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.476-482.1978
更新日期:1978-05-01 00:00:00
abstract::Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7500-7504.1993
更新日期:1993-11-01 00:00:00
abstract::The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1023-1027.1979
更新日期:1979-12-01 00:00:00
abstract::Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.300-308.1971
更新日期:1971-10-01 00:00:00
abstract::The hemA gene of Bradyrhizobium japonicum, which encodes the first enzyme in the heme biosynthetic pathway, is regulated by oxygen. Up to ninefold induction of beta-galactosidase activity is seen when cultures of B. japonicum containing either a plasmid-encoded or a chromosomally integrated hemA-lacZ fusion are shifte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.3979-3984.1995
更新日期:1995-07-01 00:00:00
abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.611-618.1996
更新日期:1996-02-01 00:00:00
abstract::Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5546-5555.1987
更新日期:1987-12-01 00:00:00
abstract::Cultural characteristics and serological relationships of pathogenic marine vibrios isolated from fish in the Pacific Northwest were studied. These organisms were compared with cultures of Vibrio anguillarum, a known fish pathogen. On the basis of morphological and cultural characteristics, the Pacific Northwest strai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1242-1247.1969
更新日期:1969-12-01 00:00:00
abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00799-16
更新日期:2017-05-09 00:00:00
abstract::Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5820-5826.1992
更新日期:1992-09-01 00:00:00
abstract::Previously, we reported that rdxB, encoding a likely membrane-bound two [4Fe-4S]-containing center, is involved in the aerobic regulation of photosystem gene expression in Rhodobacter sphaeroides 2.4.1. To further investigate the role of rdxB as well as other genes of the rdxBHIS operon on photosystem gene expression,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.12.3475-3481.2000
更新日期:2000-06-01 00:00:00
abstract::Broad-host-range IncP and IncQ plasmids have been transferred to the aerobic magnetic bacterium Aquaspirillum sp. strain AMB-1. Conjugal matings with Escherichia coli S17-1 allowed high-frequency transfer of the RK2 derivative pRK415 (4.5 x 10(-3) transconjugant per recipient cell) and the RSF1010 derivative pKT230 (3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.9.2748-2753.1992
更新日期:1992-05-01 00:00:00
abstract::Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.904-910.1963
更新日期:1963-11-01 00:00:00
abstract::Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4172-4178.1997
更新日期:1997-07-01 00:00:00
abstract::By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.334-341.1974
更新日期:1974-05-01 00:00:00
abstract::Whiteley, H. R. (University of Washington, Seattle) and N. G. McCormick. Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction. J. Bacteriol. 85:382-393. 1963.-At an acid pH, extracts of Micrococcus lactilyticus (Veillonella alcalescens) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.382-393.1963
更新日期:1963-02-01 00:00:00
abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.702-708.1964
更新日期:1964-09-01 00:00:00
abstract::Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00105-07
更新日期:2007-07-01 00:00:00
abstract::In Klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. We isolated cysteine-requiring mutants (Cys minus), and mutants (AtsS minus, AtsR minus) in which the regulation of arylsulfatase synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.29-35.1975
更新日期:1975-01-01 00:00:00
abstract::PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00217-19
更新日期:2019-07-24 00:00:00
abstract::Nahmias, André J. (Boston University School of Medicine, Boston, Mass.), and Sidney Kibrick. Inhibitory effect of heparin on herpes simplex virus. J. Bacteriol. 87:1060-1066. 1964.-A substance inhibitory to herpes simplex virus was observed during experiments with leukocyte cultures. The component in the cultures resp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1060-1066.1964
更新日期:1964-05-01 00:00:00
abstract::Four mutants specifically deficient in the activity of isocitrate lyase were independently isolated in the alkane yeast Saccharomycopsis lipolytica. Genetic analysis by means of protoplast fusion and mitotic haploidization revealed that the mutations were recessive and non-complementary at a single genetic locus, icl....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.899-908.1984
更新日期:1984-03-01 00:00:00
abstract::Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.874-881.2006
更新日期:2006-02-01 00:00:00
abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.416-421.1975
更新日期:1975-02-01 00:00:00