Role of the sporulation protein BofA in regulating activation of the Bacillus subtilis developmental transcription factor sigmaK.

Abstract:

:During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro-sigmaK to sigmaK. The results show that in the presence of BofA, SpoIVFA levels increase by greater than 20-fold, SpoIVFA is substantially stabilized, and pro-sigmaK processing is inhibited. In addition, enhanced accumulation of the SpoIVFA protein in the absence of BofA (achieved through the use of an ftsH null mutation) substantially inhibits pro-sigmaK processing. These results suggest that during growth, increased accumulation of the SpoIVFA protein inhibits the activity of SpoIVFB-GFP and regulates the activation of sigmaK.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Resnekov O

doi

10.1128/JB.181.17.5384-5388.1999

keywords:

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

5384-8

issue

17

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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