Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases.

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00

abstract：:Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...

journal_title：Journal of bacteriology

authors： Nairn BL,Eliasson OS,Hyder DR,Long NJ,Majumdar A,Chakravorty S,McDonald P,Roy A,Newton SM,Klebba PE

更新日期：2017-04-25 00:00:00

abstract：:Bacteria have evolved numerous means of survival in adverse environments with dormancy, as represented by "persistence" and the "viable but nonculturable" (VBNC) state, now recognized to be common modes for such survival. VBNC cells have been defined as cells which, induced by some stress, become nonculturable on medi...

journal_title：Journal of bacteriology

authors： Ayrapetyan M,Williams T,Oliver JD

更新日期：2018-09-24 00:00:00

abstract：:The Bacillus subtilis sacY and sacT genes encode antiterminator proteins, similar to the Escherichia coli bglG gene product and required for transcription of sucrose metabolism genes. A Tn10 insertion into bglP (formerly sytA) has been previously identified as restoring sucrose utilization to a strain with deletions o...

journal_title：Journal of bacteriology

authors： Le Coq D,Lindner C,Krüger S,Steinmetz M,Stülke J

更新日期：1995-03-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

journal_title：Journal of bacteriology

authors： Hahn J,Dubnau D

更新日期：1985-06-01 00:00:00

abstract：:Differential expression of genes within the puf photosynthesis operon of Rhodobacter capsulatus is achieved primarily through marked segmental differences in stability within the polycistronic puf operon transcripts. The comparatively stable pufBA segment of these transcripts outlives the labile pufLMX segment and acc...

journal_title：Journal of bacteriology

authors： Chen CY,Belasco JG

更新日期：1990-08-01 00:00:00

abstract：:A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...

journal_title：Journal of bacteriology

authors： Deretic V,Konyecsni WM

更新日期：1989-07-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00

abstract：:The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

journal_title：Journal of bacteriology

authors： Tanabe H,Goldstein J,Yang M,Inouye M

更新日期：1992-06-01 00:00:00

abstract：:Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyme synthesis. J. Bacteriol. 84:996-1006. 1962.-The requirements for the formation of tryptophanase and tryptophan synthetase in Escherichia coli ...

journal_title：Journal of bacteriology

authors： FREUNDLICH M,LICHSTEIN HC

更新日期：1962-11-01 00:00:00

abstract：:The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...

journal_title：Journal of bacteriology

authors： Belitsky BR,Gustafsson MC,Sonenshein AL,Von Wachenfeldt C

更新日期：1997-09-01 00:00:00

abstract：:A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medi...

journal_title：Journal of bacteriology

authors： Daron HH

更新日期：1967-02-01 00:00:00

abstract：:By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of te...

journal_title：Journal of bacteriology

authors： Smith JM,Umbarger HE

更新日期：1977-12-01 00:00:00

abstract：:Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...

journal_title：Journal of bacteriology

authors： STEVENS JG,GROMAN NB

更新日期：1964-02-01 00:00:00

abstract：:The structures of the lipooligosaccharides (LOSs) from Haemophilus ducreyi ITM 5535 and ITM 3147 and a fresh clinical isolate, ACY1, have been investigated. Oligosaccharides were obtained from phenol-water-extracted LOS by mild acid hydrolysis and were studied by methylation analysis, fast atom bombardment and electro...

journal_title：Journal of bacteriology

authors： Schweda EK,Jonasson JA,Jansson PE

更新日期：1995-09-01 00:00:00

abstract：:The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

journal_title：Journal of bacteriology

authors： Mukerjea R,Ghosh P

更新日期：2013-10-01 00:00:00

abstract：:Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....

journal_title：Journal of bacteriology

authors： Rebeil R,Ernst RK,Jarrett CO,Adams KN,Miller SI,Hinnebusch BJ

更新日期：2006-02-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

journal_title：Journal of bacteriology

authors： KIMLER A

更新日期：1961-07-01 00:00:00

abstract：:Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

journal_title：Journal of bacteriology

authors： Kuroda MI,Henner D,Yanofsky C

更新日期：1988-07-01 00:00:00

abstract：:A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

journal_title：Journal of bacteriology

authors： Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

更新日期：1998-05-01 00:00:00

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...

journal_title：Journal of bacteriology

authors： Beggs WH,Rogers P

更新日期：1966-05-01 00:00:00

abstract：:The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

journal_title：Journal of bacteriology

authors： Rowland GC,Giffard PM,Booth IR

更新日期：1985-11-01 00:00:00

abstract：:Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...

journal_title：Journal of bacteriology

authors： Stubbendieck RM,Straight PD

更新日期：2016-07-28 00:00:00

abstract：:Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

journal_title：Journal of bacteriology

authors： Morty RE,Fülöp V,Andrews NW

更新日期：2002-06-01 00:00:00

abstract：:Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...

journal_title：Journal of bacteriology

authors： BEPPU M,ARIMA K

更新日期：1964-07-01 00:00:00