Degradation of pufLMX mRNA in Rhodobacter capsulatus is initiated by nonrandom endonucleolytic cleavage.

abstract：:d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

journal_title：Journal of bacteriology

authors： Boulter JR,Gielow WO

更新日期：1973-02-01 00:00:00

abstract：:Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and ...

journal_title：Journal of bacteriology

authors： Hempel AM,Wang SB,Letek M,Gil JA,Flärdh K

更新日期：2008-11-01 00:00:00

abstract：:Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

journal_title：Journal of bacteriology

authors： Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

更新日期：2010-11-01 00:00:00

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...

journal_title：Journal of bacteriology

authors： Yamaguchi H,Kanda Y,Iwata K

更新日期：1974-10-01 00:00:00

abstract：:The class 1 outer membrane protein encoded by the porA gene of Neisseria meningitidis is a candidate for a vaccine against meningococcal infection. The expression of class 1 outer membrane protein displays phase variation between three expression levels. Northern (RNA) blot and primer extension analysis revealed that ...

journal_title：Journal of bacteriology

authors： van der Ende A,Hopman CT,Zaat S,Essink BB,Berkhout B,Dankert J

更新日期：1995-05-01 00:00:00

abstract：:The genomes of most strains of Salmonella and Escherichia coli are highly conserved. In contrast, all 136 wild-type strains of Salmonella enterica serovar Typhi analyzed by partial digestion with I-CeuI (an endonuclease which cuts within the rrn operons) and pulsed-field gel electrophoresis and by PCR have rearrangeme...

journal_title：Journal of bacteriology

authors： Kothapalli S,Nair S,Alokam S,Pang T,Khakhria R,Woodward D,Johnson W,Stocker BA,Sanderson KE,Liu SL

更新日期：2005-04-01 00:00:00

abstract：:Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

journal_title：Journal of bacteriology

authors： Butler CA,Gotschlich EC

更新日期：1991-09-01 00:00:00

abstract：:The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

journal_title：Journal of bacteriology

authors： den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

更新日期：2008-07-01 00:00:00

abstract：:Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...

journal_title：Journal of bacteriology

authors： Miller JR,Ohren J,Sarver RW,Mueller WT,de Dreu P,Case H,Thanabal V

更新日期：2007-11-01 00:00:00

abstract：:The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

journal_title：Journal of bacteriology

authors： Szurmant H,Mohan MA,Imus PM,Hoch JA

更新日期：2007-04-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

journal_title：Journal of bacteriology

authors： Choi SH,Greenberg EP

更新日期：1992-06-01 00:00:00

abstract：:To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...

journal_title：Journal of bacteriology

authors： Ormonde P,Hörstedt P,O'Toole R,Milton DL

更新日期：2000-04-01 00:00:00

abstract：:Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

journal_title：Journal of bacteriology

authors： Sung YC,Anderson PM,Fuchs JA

更新日期：1987-11-01 00:00:00

abstract：:Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

journal_title：Journal of bacteriology

authors： Williams RM,Rimsky S,Buc H

更新日期：1996-08-01 00:00:00

abstract：:Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

journal_title：Journal of bacteriology

authors： ELLNER PD,BOHAN CD

更新日期：1962-02-01 00:00:00

abstract：:Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...

journal_title：Journal of bacteriology

authors： ALTENBERN RA

更新日期：1963-03-01 00:00:00

abstract：:A mutant of the yeast Saccharomyces cerevisiae that is deficient in pyruvate kinase activity has been isolated. The mutant strain is capable of growth when supplied with lactate as the carbon source but not capable of growth when supplied with dextrose or other fermentable sugars or glycerol as the carbon source. Gene...

journal_title：Journal of bacteriology

authors： Sprague GF Jr

更新日期：1977-04-01 00:00:00

abstract：:Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...

journal_title：Journal of bacteriology

authors： Magill CW,Sabina RL,Garber TL,Magill JM

更新日期：1982-03-01 00:00:00

abstract：:In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

journal_title：Journal of bacteriology

authors： Raju KK,Gautam S,Sharma A

更新日期：2006-08-01 00:00:00

abstract：:Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

journal_title：Journal of bacteriology

authors： Kuske CR,Kirkpatrick BC

更新日期：1990-03-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00

abstract：:The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

journal_title：Journal of bacteriology

authors： Szabo M,Maskell D,Butler P,Love J,Moxon R

更新日期：1992-11-01 00:00:00

abstract：:Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

journal_title：Journal of bacteriology

authors： Yolton DP,Pope L,Williams MG,Rode LJ

更新日期：1968-01-01 00:00:00

abstract：:Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution o...

journal_title：Journal of bacteriology

authors： Fukazawa Y,Shinoda T,Tsuchiya T

更新日期：1969-06-01 00:00:00

abstract：:Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...

journal_title：Journal of bacteriology

authors： Chang MT,Raper KB

更新日期：1981-09-01 00:00:00

abstract：:Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

journal_title：Journal of bacteriology

authors： Hahn J,Dubnau D

更新日期：1985-06-01 00:00:00

abstract：:Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by ceru...

journal_title：Journal of bacteriology

authors： Campbell TB,Lueking DR

更新日期：1983-02-01 00:00:00