Probing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives.

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

journal_title：Journal of bacteriology

authors： Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

更新日期：2010-09-01 00:00:00

abstract：:A pentachlorophenol (PCP) hydroxylase which catalyzed the conversion of PCP to 2,3,5,6-tetrachlorohydroquinone and released iodide from triiodophenol in the presence of NADPH and oxygen was identified. The enzyme was purified by protamine sulfate precipitation, ammonium sulfate precipitation, hydrophobic chromatograph...

journal_title：Journal of bacteriology

authors： Xun L,Orser CS

更新日期：1991-07-01 00:00:00

abstract：:We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the xylAB regulatory region to the lipase gene from Staphylococcus hyicus were constructed. Repression of lipase activity depend...

journal_title：Journal of bacteriology

authors： Sizemore C,Wieland B,Götz F,Hillen W

更新日期：1992-05-01 00:00:00

abstract：:An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...

journal_title：Journal of bacteriology

authors： Buswell JA

更新日期：1975-12-01 00:00:00

abstract：:We demonstrate that the expression of hem genes in Rhodobacter capsulatus is transcriptionally repressed in response to the exogenous addition of heme. A high-copy suppressor screen for regulators of hem gene expression resulted in the identification of an LysR-type transcriptional regulator, called HbrL, that regulat...

journal_title：Journal of bacteriology

authors： Smart JL,Bauer CE

更新日期：2006-02-01 00:00:00

abstract：:Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...

journal_title：Journal of bacteriology

authors： Wong B,Leeson S,Grindle S,Magee B,Brooks E,Magee PT

更新日期：1995-06-01 00:00:00

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:Ureolytic clinical isolates of Providencia stuartii, Salmonella spp., and some Escherichia coli strains contain large urease-encoding plasmids. Expression of urease activity from these isolates is induced at least 20-fold by urea. In order to facilitate studies on the regulatory mechanism controlling this urea-inducib...

journal_title：Journal of bacteriology

authors： D'Orazio SE,Collins CM

更新日期：1993-06-01 00:00:00

abstract：:Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

journal_title：Journal of bacteriology

authors： Tominaga A,Kobayashi Y

更新日期：1978-09-01 00:00:00

abstract：:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

journal_title：Journal of bacteriology

authors： Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

更新日期：1999-10-01 00:00:00

abstract：:Besides producing thymine-requiring mutants (thy), trimethoprim (TMP) cured the mini-ColE1 replicon pML21 at an appreciable frequency. The cured Escherichia coli K-12 cells behaved like polA mutants by failing to support the stable maintenance of the ColE1 plasmid. The mini-F replicon pSC138, which was lacking all thr...

journal_title：Journal of bacteriology

authors： Mukhopadhyay P,Palchaudhuri S

更新日期：1982-05-01 00:00:00

abstract：:Sphingobium japonicum UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of gamma-HCH to beta-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutage...

journal_title：Journal of bacteriology

authors： Endo R,Ohtsubo Y,Tsuda M,Nagata Y

更新日期：2007-05-01 00:00:00

abstract：:Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

journal_title：Journal of bacteriology

authors： Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

更新日期：2005-07-01 00:00:00

abstract：:The morphology, on MacConkey's agar, of colonies of the bacteria commonly found in enteric specimens is sufficiently characteristic and distinctive, when observed with transmitted oblique illumination and a low-power stereoscopic microscope, to enable identification at the generic (and occasionally the species) level ...

journal_title：Journal of bacteriology

authors： Finkelstein RA,Punyashthiti K

更新日期：1967-06-01 00:00:00

abstract：:Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...

journal_title：Journal of bacteriology

authors： Börnke F,Hajirezaei M,Sonnewald U

更新日期：2001-04-01 00:00:00

abstract：:We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...

journal_title：Journal of bacteriology

authors： Deng W,Burland V,Plunkett G 3rd,Boutin A,Mayhew GF,Liss P,Perna NT,Rose DJ,Mau B,Zhou S,Schwartz DC,Fetherston JD,Lindler LE,Brubaker RR,Plano GV,Straley SC,McDonough KA,Nilles ML,Matson JS,Blattner FR,Perry RD

更新日期：2002-08-01 00:00:00

abstract：:Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...

journal_title：Journal of bacteriology

authors： Hase T

更新日期：1983-05-01 00:00:00

abstract：:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

journal_title：Journal of bacteriology

authors： Shirai Y,Akiyama Y,Ito K

更新日期：1996-02-01 00:00:00

abstract：:A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...

journal_title：Journal of bacteriology

authors： Gabe JD,Dragon E,Chang RJ,McCaman MT

更新日期：1998-01-01 00:00:00

abstract：:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

journal_title：Journal of bacteriology

authors： Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

更新日期：1998-10-01 00:00:00

abstract：:The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...

journal_title：Journal of bacteriology

authors： Shively JM,Decker GL,Greenawalt JW

更新日期：1970-02-01 00:00:00

abstract：:Five transposon Tn5 mutants of the procaryote Myxococcus xanthus had been shown previously to be defective in lipopolysaccharide biosynthesis (J. M. Fink,-M. Kalos, and J. F. Zissler, J. Bacteriol. 171:2033-2041, 1989). These mutants were studied for possible defects in gliding motility and multicellular development. ...

journal_title：Journal of bacteriology

authors： Fink JM,Zissler JF

更新日期：1989-04-01 00:00:00

abstract：:Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...

journal_title：Journal of bacteriology

authors： Dong C,Elsen S,Swem LR,Bauer CE

更新日期：2002-05-01 00:00:00

abstract：:Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

journal_title：Journal of bacteriology

authors： Satta G,Fontana R,Canepari P,Botta G

更新日期：1979-02-01 00:00:00

abstract：:Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss of the ability to respond to enhancer binding proteins. In this work PCR mutagenesis and genetic screens were used to identify a small patch, f...

journal_title：Journal of bacteriology

authors： Syed A,Gralla JD

更新日期：1998-11-01 00:00:00

abstract：:The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fraction...

journal_title：Journal of bacteriology

authors： Barlati S,Majerfeld I

更新日期：1970-02-01 00:00:00

abstract：:Using yeast two-hybrid screens, we have defined an interaction group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili. The six proteins are TraH, TraF, TraW, TraU, TrbI, and TrbB. Except for TrbI, these proteins were all identified as hallmarks of F-like type IV secretion sys...

journal_title：Journal of bacteriology

authors： Harris RL,Silverman PM

更新日期：2004-08-01 00:00:00

abstract：:Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...

journal_title：Journal of bacteriology

authors： Yoshida K,Ekstedt RD

更新日期：1968-11-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00