Identification of phthiodiolone ketoreductase, an enzyme required for production of mycobacterial diacyl phthiocerol virulence factors.

Abstract:

:Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step required for diacyl (phenol)phthiocerol ester production, i.e., the reduction of the keto group of (phenol)phthiodiolones. We utilized comparative genomics to identify phthiodiolone ketoreductase gene candidates and provide a genetic analysis demonstrating gene function for two of these candidates. Moreover, we present data confirming the existence of a diacyl phthiotriol intermediate in diacyl phthiocerol biosynthesis. We also elucidate the mechanism underlying diacyl phthiocerol deficiency in some mycobacteria, such as Mycobacterium ulcerans and Mycobacterium kansasii. Overall, our findings shed additional light on the biosynthesis of an important group of mycobacterial lipids involved in virulence.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

doi

10.1128/JB.187.14.4760-4766.2005

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

4760-6

issue

14

eissn

0021-9193

issn

1098-5530

pii

187/14/4760

journal_volume

187

pub_type

杂志文章
  • Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

    abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6083-6094.2003

    authors: Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

    更新日期:2003-10-01 00:00:00

  • CHANGES IN MOUSE PNEUMONITIS AGENT ASSOCIATED WITH DEVELOPMENT OF RESISTANCE TO CHLORTETRACYCLINE.

    abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.17-22.1965

    authors: MOULDER JW,NOVOSEL DL,TRIBBY II

    更新日期:1965-01-01 00:00:00

  • Characterization of EvgAS-YdeO-GadE branched regulatory circuit governing glutamate-dependent acid resistance in Escherichia coli.

    abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7378-7389.2004

    authors: Ma Z,Masuda N,Foster JW

    更新日期:2004-11-01 00:00:00

  • Signal transduction protein PII phosphatase PphA is required for light-dependent control of nitrate utilization in synechocystis sp. strain PCC 6803.

    abstract::Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.19.6683-6690.2005

    authors: Kloft N,Forchhammer K

    更新日期:2005-10-01 00:00:00

  • INTESTINAL BACTERIAL FLORA AND TRANSIT TIME OF THREE NEOTROPICAL BAT SPECIES.

    abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.375-379.1965

    authors: KLITE PD

    更新日期:1965-08-01 00:00:00

  • Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae?

    abstract::The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.25-31.1985

    authors: Hackstadt T,Todd WJ,Caldwell HD

    更新日期:1985-01-01 00:00:00

  • Production and release of peptidoglycan and wall teichoic acid polymers in pneumococci treated with beta-lactam antibiotics.

    abstract::Autolysin-defective pneumococci treated with inhibitory concentrations of penicillin and other beta-lactam antibiotics continued to produce non-cross-linked peptidoglycan and cell wall teichoic acid polymers, the majority of which were released into the surrounding medium. The released cell wall polymers were those sy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.507-513.1984

    authors: Fischer H,Tomasz A

    更新日期:1984-02-01 00:00:00

  • Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7.

    abstract::Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.5.1783-1791.2005

    authors: Wick LM,Qi W,Lacher DW,Whittam TS

    更新日期:2005-03-01 00:00:00

  • Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus.

    abstract::In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6115-6122.2002

    authors: Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

    更新日期:2002-11-01 00:00:00

  • Analytical studies on regeneration of protoplasts of Geotrichum candidum by quantitative thin-layer-agar plating.

    abstract::A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.256-263.1969

    authors: Fukui K,Sagara Y,Yoshida N,Matsuoka T

    更新日期:1969-04-01 00:00:00

  • Iron is required to relieve inhibitory effects on NifL on transcriptional activation by NifA in Klebsiella pneumoniae.

    abstract::In Klebsiella pneumoniae, products of the nitrogen fixation nifLA operon regulate transcription of the other nif operons. NifA activates transcription by sigma54-holoenzyme. In vivo, NifL antagonizes the action of NifA under aerobic conditions or in the presence of combined nitrogen. In contrast to a previous report, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.15.4679-4687.1996

    authors: Schmitz RA,He L,Kustu S

    更新日期:1996-08-01 00:00:00

  • Polar targeting of DivIVA in Bacillus subtilis is not directly dependent on FtsZ or PBP 2B.

    abstract::DivIVA is involved in Bacillus subtilis cell division and is located at the cell poles. Previous experiments suggested that the cell division proteins FtsZ and PBP 2B are required for polar targeting of DivIVA. By using outgrowing spores, we show that DivIVA accumulates at the cell poles independent of the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.2.693-697.2003

    authors: Hamoen LW,Errington J

    更新日期:2003-01-01 00:00:00

  • Altered muropeptide composition in Staphylococcus aureus strains with an inactivated femA locus.

    abstract::Tn551 inactivation of femA, a factor involved in methicillin resistance of Staphylococcus aureus, caused the production of peptidoglycan in which the fraction of monoglycyl- and serine-containing muropeptides was increased at the expense of pentaglycyl muropeptides. femA mutants have a specific block in the biosynthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2779-2782.1993

    authors: de Jonge BL,Sidow T,Chang YS,Labischinski H,Berger-Bachi B,Gage DA,Tomasz A

    更新日期:1993-05-01 00:00:00

  • Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363.

    abstract::Carbon catabolite control protein A (CcpA) is the main regulator involved in carbon catabolite repression in gram-positive bacteria. Time series gene expression analyses of Lactococcus lactis MG1363 and L. lactis MG1363DeltaccpA using DNA microarrays were used to define the CcpA regulon of L. lactis. Based on a compar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01013-06

    authors: Zomer AL,Buist G,Larsen R,Kok J,Kuipers OP

    更新日期:2007-02-01 00:00:00

  • Upstream migration of Xylella fastidiosa via pilus-driven twitching motility.

    abstract::Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzlin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5560-5567.2005

    authors: Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HC

    更新日期:2005-08-01 00:00:00

  • Correlation of 16S ribosomal DNA signature sequences with temperature-dependent growth rates of mesophilic and psychrotolerant strains of the Bacillus cereus group.

    abstract::Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2624-2630.1999

    authors: Prüss BM,Francis KP,von Stetten F,Scherer S

    更新日期:1999-04-01 00:00:00

  • Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B.

    abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.35-41.1985

    authors: Goldberg SL,Murphy JR

    更新日期:1985-04-01 00:00:00

  • Mutational analysis of the TonB1 energy coupler of Pseudomonas aeruginosa.

    abstract::Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.6.1503-1513.2002

    authors: Zhao Q,Poole K

    更新日期:2002-03-01 00:00:00

  • The major facilitator superfamily-type transporter YmfE and the multidrug-efflux activator Mta mediate bacillibactin secretion in Bacillus subtilis.

    abstract::High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00464-08

    authors: Miethke M,Schmidt S,Marahiel MA

    更新日期:2008-08-01 00:00:00

  • Two roles for the DNA recognition site of the Klebsiella aerogenes nitrogen assimilation control protein.

    abstract::The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.3.578-585.1998

    authors: Pomposiello PJ,Janes BK,Bender RA

    更新日期:1998-02-01 00:00:00

  • Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis.

    abstract::An earlier study of the regulation of glutamate synthase (GOGAT) in Bacillus subtilis (Deshpande et al., Bichem. Biophys. Res. Commun. 95:55--60, 1980) revealed an inverse relationship between the specific activity of this essential ammonia-assimilatory enzyme and the intracellular pool of glutamine: GOGAT activity de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.768-774.1981

    authors: Deshpande KL,Katze JR,Kane JF

    更新日期:1981-02-01 00:00:00

  • UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.

    abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05337-11

    authors: Coyne MJ,Fletcher CM,Reinap B,Comstock LE

    更新日期:2011-10-01 00:00:00

  • MICROBIAL METABOLISM OF AROMATIC COMPOUNDS. I. DECOMPOSITION OF PHENOLIC COMPOUNDS AND AROMATIC HYDROCARBONS BY PHENOL-ADAPTED BACTERIA.

    abstract::Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.4.910-919.1964

    authors: TABAK HH,CHAMBERS CW,KABLER PW

    更新日期:1964-04-01 00:00:00

  • Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen.

    abstract::In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.755-762.1976

    authors: Schmidt G,Mayer H,Mäkelä PH

    更新日期:1976-08-01 00:00:00

  • Chromosome condensation in the absence of the non-SMC subunits of MukBEF.

    abstract::MukBEF is a bacterial SMC (structural maintenance of chromosome) complex required for chromosome partitioning in Escherichia coli. We report that overproduction of MukBEF results in marked chromosome condensation. This condensation is rapid and precedes the effects of overproduction on macromolecular synthesis. Conden...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00313-06

    authors: Wang Q,Mordukhova EA,Edwards AL,Rybenkov VV

    更新日期:2006-06-01 00:00:00

  • Effects of homocitrate, homocitrate lactone, and fluorohomocitrate on nitrogenase in NifV- mutants of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5403-5405.1991

    authors: Madden MS,Paustian TD,Ludden PW,Shah VK

    更新日期:1991-09-01 00:00:00

  • L-serine degradation in Escherichia coli K-12: a combination of L-serine, glycine, and leucine used as a source of carbon.

    abstract::Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.2.777-782.1982

    authors: Newman EB,Walker C

    更新日期:1982-08-01 00:00:00

  • Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.

    abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.861-865.1974

    authors: Futai M

    更新日期:1974-11-01 00:00:00

  • Heat reactivation of the alpha-hemolytic, dermonecrotic, lethal activities of crude and purified staphylococcal alpha-toxin.

    abstract::Manohar, M. (University of Minnesota, St. Paul), S. Kumar, and R. K. Lindorfer. Heat reactivation of the alpha-hemolytic, dermonecrotic, and lethal activities of crude and purified staphylococcal alpha-toxin. J. Bacteriol. 91:1681-1685. 1966.-Crude staphylococcal toxin loses its alpha-hemolytic activity more rapidly a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1681-1685.1966

    authors: Manohar M,Kumar S,Lindorfer RK

    更新日期:1966-05-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.676-681.1972

    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00