Abstract:
:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus. Disruption of the chromosomal amfR gene resulted in complete abolition of aerial mycelium formation, indicating that amfR is essential for the onset of morphogenesis. Cloning and nucleotide sequencing of the region upstream of amfR predicted an operon consisting of orf5, orf4, and amfR. Consistent with this idea, Northern blotting and S1 mapping analyses suggested that these three genes were cotranscribed mainly by a promoter (PORF5) in front of orf5. Furthermore, PORF5 was active only in the presence of A-factor, indicating that it is A-factor dependent. Gel mobility shift assays showed the presence of a protein (AdpB) able to bind PORF5 in the cell extract from an A-factor-deficient mutant but not from the wild-type strain. AdpB was purified to homogeneity and found to bind specifically to the region from -72 to -44 bp with respect to the transcriptional start point. Runoff transcriptional analysis of PORF5 with purified AdpB and an RNA polymerase complex isolated from vegetative mycelium showed that AdpB repressed the transcription in a concentration-dependent manner. It is thus apparent that AmfR as a switch for aerial mycelium formation and AdpB as a repressor for amfR are members in the A-factor regulatory cascade, leading to morphogenesis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi Sdoi
10.1128/JB.180.19.5085-5093.1998subject
Has Abstractpub_date
1998-10-01 00:00:00pages
5085-93issue
19eissn
0021-9193issn
1098-5530journal_volume
180pub_type
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doi:10.1128/JB.180.12.3187-3196.1998
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doi:10.1128/JB.02090-12
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.173.3.1360-1362.1991
更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-09-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2015-10-26 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.173.16.5220-5223.1991
更新日期:1991-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3587-3588.1995
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-03-01 00:00:00
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更新日期:1995-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4829-4832.1992
更新日期:1992-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1425-1430.1968
更新日期:1968-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4468-4476.2001
更新日期:2001-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.775-779.1981
更新日期:1981-02-01 00:00:00
abstract::The isolation and some properties of two mutants of Streptococcus faecalis ATCC 9790 (S. faecium) which autolyze at a much slower rate than the wild type are described. Compared with the wild type, mutant E71 autolyzed more slowly, contained less active but more latent autolysin in the isolated wall fraction, and poss...
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pub_type: 杂志文章
doi:10.1128/JB.109.1.423-431.1972
更新日期:1972-01-01 00:00:00