Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri.

abstract：:The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...

journal_title：Journal of bacteriology

authors： Brakhage AA,Putzer H,Shazand K,Röschenthaler RJ,Grunberg-Manago M

更新日期：1989-02-01 00:00:00

abstract：:Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

journal_title：Journal of bacteriology

authors： Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

更新日期：1982-07-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC 1.2.1.12) and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...

journal_title：Journal of bacteriology

authors： Maurer KH,Pfeiffer F,Zehender H,Mecke D

更新日期：1983-02-01 00:00:00

abstract：:The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...

journal_title：Journal of bacteriology

authors： Montrone M,Eisenbach M,Oesterhelt D,Marwan W

更新日期：1998-07-01 00:00:00

abstract：:A specific gamma-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a K(m) of 12 muM and a V(max) of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the a...

journal_title：Journal of bacteriology

authors： Kahane S,Levitz R,Halpern YS

更新日期：1978-08-01 00:00:00

abstract：:Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...

journal_title：Journal of bacteriology

authors： Pfister A,DeVries JK,Maas WK

更新日期：1976-07-01 00:00:00

abstract：:The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated. Slime production in culture seemed optimal when adequate amounts of carbohydrate were present and under conditions of either high osmotic pressure or inadequate protein supply. The polysaccharide...

journal_title：Journal of bacteriology

authors： Evans LR,Linker A

更新日期：1973-11-01 00:00:00

abstract：:A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

journal_title：Journal of bacteriology

authors： Rich JJ,Willis DK

更新日期：1997-04-01 00:00:00

abstract：:The TspO outer membrane protein of Rhodobacter sphaeroides has been shown to be involved in controlling the transcription of a number of genes which encode enzymes involved in photopigment biosynthesis and the puc operon. The display of regulated genes appears identical to those genes encompassing the PpsR/AppA repres...

journal_title：Journal of bacteriology

authors： Zeng X,Kaplan S

更新日期：2001-11-01 00:00:00

abstract：:Two sensor proteins, NarX and NarQ, mediate nitrate regulation of anaerobic respiratory gene expression. Either of these sensors is sufficient to signal the presence of nitrate to the response regulator protein, NarL, a transcriptional activator and repressor. Two observations suggested the existence of a second respo...

journal_title：Journal of bacteriology

authors： Rabin RS,Stewart V

更新日期：1993-06-01 00:00:00

abstract：:Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...

journal_title：Journal of bacteriology

authors： Bojovic B,Djordjevic G,Banina A,Topisirovic L

更新日期：1994-11-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00

abstract：:It has been suggested that catalase-peroxidase plays an important role in several aspects of mycobacterial metabolism and is a virulence factor in the main pathogenic mycobacteria. In this investigation, we studied genes encoding for this protein in the fast-growing opportunistic pathogen Mycobacterium fortuitum. Nucl...

journal_title：Journal of bacteriology

authors： Menéndez MC,Ainsa JA,Martín C,García MJ

更新日期：1997-11-01 00:00:00

abstract：:A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...

journal_title：Journal of bacteriology

authors： Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek C

更新日期：1987-12-01 00:00:00

abstract：:Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...

journal_title：Journal of bacteriology

authors： Basu J,Chattopadhyay R,Kundu M,Chakrabarti P

更新日期：1992-07-01 00:00:00

abstract：:Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...

journal_title：Journal of bacteriology

authors： Zheng RC,Lu XF,Tomita H,Hachisuka SI,Zheng YG,Atomi H

更新日期：2021-01-19 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...

journal_title：Journal of bacteriology

authors： Oliver DB

更新日期：1985-01-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:Several biochemical parameters, including that of polyamine content, accompanying the growth of the cyanobacterium Anacystis nidulans were studied. At all stages of growth under autotrophic conditions, the organisms were found to be rich in spermidine and lacking in spermine, as is typical of procaryotic organisms. Th...

journal_title：Journal of bacteriology

authors： Ramakrishna S,Guarino L,Cohen SS

更新日期：1978-06-01 00:00:00

abstract：:Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

journal_title：Journal of bacteriology

authors： Peschek GA

更新日期：1983-01-01 00:00:00

abstract：:To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...

journal_title：Journal of bacteriology

authors： Ormonde P,Hörstedt P,O'Toole R,Milton DL

更新日期：2000-04-01 00:00:00

abstract：:After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

journal_title：Journal of bacteriology

authors： Sargent MG,Bennett MF

更新日期：1986-04-01 00:00:00

abstract：:Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...

journal_title：Journal of bacteriology

authors： Altendorf K,Epstein W,Löhmann A

更新日期：1986-04-01 00:00:00

abstract：:Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...

journal_title：Journal of bacteriology

authors： Araújo Alves L,Ganguly T,Mattos-Graner RO,Kajfasz J,Harth-Chu EN,Lemos JA,Abranches J

更新日期：2018-11-06 00:00:00

abstract：:Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...

journal_title：Journal of bacteriology

authors： NISHIDA S,NAKAGAWARA G

更新日期：1965-04-01 00:00:00

abstract：:In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

journal_title：Journal of bacteriology

authors： Rice LB,Carias LL,Hutton-Thomas R,Rudin S

更新日期：2007-05-01 00:00:00

abstract：:Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...

journal_title：Journal of bacteriology

authors： Ramos JL,Duque E,Huertas MJ,Haïdour A

更新日期：1995-07-01 00:00:00