Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.

Abstract:

:After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a previously reported observation that additional copies of the binding region, introduced into the chromosome using an integrative plasmid, were also predominantly particle bound. Subsections of the original plasmid insertion were cloned into the integrative plasmid and introduced into B. subtilis, in which they became tandemly reiterated under appropriate selective conditions. HaeIII sites in the vector, flanking each insertion, were used to excise the latter for subsequent tests of particle association. Examination of 10 strains containing subsections of the original 5.2-kilobase-pair region showed that the binding region was confined to 283 base pairs. This was confirmed by dissection in vitro of a larger, isolated, particle-bound sequence. The nucleotide sequence of a 1,300-base-pair region that contained this site was determined. The entire region had a notably high A + T content and was deficient in open reading frames for transcription.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sargent MG,Bennett MF

doi

10.1128/jb.166.1.38-43.1986

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

38-43

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

166

pub_type

杂志文章
  • Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI.

    abstract::Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.335-342.1962

    authors: REBERS PA,HURWITZ E,HEIDELBERGER M,ESTRADA-PARRA S

    更新日期:1962-02-01 00:00:00

  • CsgA, an extracellular protein essential for Myxococcus xanthus development.

    abstract::CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5299-5306.1990

    authors: Shimkets LJ,Rafiee H

    更新日期:1990-09-01 00:00:00

  • Transcriptional analysis of pqqD and study of the regulation of pyrroloquinoline quinone biosynthesis in Methylobacterium extorquens AM1.

    abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.206-211.1995

    authors: Ramamoorthi R,Lidstrom ME

    更新日期:1995-01-01 00:00:00

  • Trimethoprim-produced F-specific insertion mutations in Escherichia coli K-12.

    abstract::Besides producing thymine-requiring mutants (thy), trimethoprim (TMP) cured the mini-ColE1 replicon pML21 at an appreciable frequency. The cured Escherichia coli K-12 cells behaved like polA mutants by failing to support the stable maintenance of the ColE1 plasmid. The mini-F replicon pSC138, which was lacking all thr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.755-761.1982

    authors: Mukhopadhyay P,Palchaudhuri S

    更新日期:1982-05-01 00:00:00

  • Assimilatory sulfur metabolism in marine microorganisms: characteristics and regulation of sulfate transport in Pseudomonas halodurans and Alteromonas luteo-violaceus.

    abstract::Sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in Pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. Subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. Only methionine failed to regulate sulfat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.2.340-349.1981

    authors: Cuhel RL,Taylor CD,Jannasch HW

    更新日期:1981-08-01 00:00:00

  • The C-terminus of substrates is critical but not sufficient for their degradation by the Pseudomonas aeruginosa CtpA protease.

    abstract::Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00174-20

    authors: Chung S,Darwin AJ

    更新日期:2020-06-01 00:00:00

  • EnvZ functions through OmpR to control porin gene expression in Escherichia coli K-12.

    abstract::The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.439-441.1988

    authors: Slauch JM,Garrett S,Jackson DE,Silhavy TJ

    更新日期:1988-01-01 00:00:00

  • Stabilization of colicin E2 by bovine serum albumin.

    abstract::Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1136-1137.1969

    authors: Mitsui E,Mizuno D

    更新日期:1969-11-01 00:00:00

  • The bZip transcription factor Cap1p is involved in multidrug resistance and oxidative stress response in Candida albicans.

    abstract::Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.3.700-708.1999

    authors: Alarco AM,Raymond M

    更新日期:1999-02-01 00:00:00

  • A cytochrome cbb3 (cytochrome c) terminal oxidase in Azospirillum brasilense Sp7 supports microaerobic growth.

    abstract::Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5689-5696.1998

    authors: Marchal K,Sun J,Keijers V,Haaker H,Vanderleyden J

    更新日期:1998-11-01 00:00:00

  • Genes for the establishment and maintenance of lysogeny by the temperate coliphage 186.

    abstract::To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5286-5288.1993

    authors: Lamont I,Richardson H,Carter DR,Egan JB

    更新日期:1993-08-01 00:00:00

  • Ribosomal features essential for tna operon induction: tryptophan binding at the peptidyl transferase center.

    abstract::Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01869-06

    authors: Cruz-Vera LR,New A,Squires C,Yanofsky C

    更新日期:2007-04-01 00:00:00

  • Isolation and characterization of yeast monomorphic mutants of Candida albicans.

    abstract::A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.8.2318-2325.1994

    authors: Elorza MV,Sentandreu R,Ruiz-Herrera J

    更新日期:1994-04-01 00:00:00

  • Streptococcus pneumoniae uses glutathione to defend against oxidative stress and metal ion toxicity.

    abstract::The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01393-12

    authors: Potter AJ,Trappetti C,Paton JC

    更新日期:2012-11-01 00:00:00

  • Metabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.

    abstract::In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.293-299.1971

    authors: Oliver EJ,Mortlock RP

    更新日期:1971-10-01 00:00:00

  • Ribosomes of the extremely thermophilic eubacterium Thermotoga maritima are uniquely insensitive to the miscoding-inducing action of aminoglycoside antibiotics.

    abstract::Poly(U)- and poly(UG)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium Thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees C) close to the physiological optimum (80 degrees C) for cell growth and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4353-4360.1988

    authors: Londei P,Altamura S,Huber R,Stetter KO,Cammarano P

    更新日期:1988-09-01 00:00:00

  • Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.

    abstract::The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs were identified distal to this locus. However, only two polypeptides of apparent molecular masses 16 and 56 kilodaltons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5035-5045.1987

    authors: Jayaswal RK,Veluthambi K,Gelvin SB,Slightom JL

    更新日期:1987-11-01 00:00:00

  • Conjugative transfer of Tn916 in Enterococcus faecalis: trans activation of homologous transposons.

    abstract::Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.22.7136-7141.1991

    authors: Flannagan SE,Clewell DB

    更新日期:1991-11-01 00:00:00

  • The lrp gene product regulates expression of lysU in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2779-2784.1992

    authors: Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

    更新日期:1992-05-01 00:00:00

  • Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.

    abstract::Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1623-1630.1996

    authors: Costello GM,Vipond R,MacIntyre S

    更新日期:1996-03-01 00:00:00

  • A mutation in the Neisseria gonorrhoeae rfaD homolog results in altered lipooligosaccharide expression.

    abstract::The gonococcal lsi-6 locus was cloned and shown by DNA sequence analysis to have homology with the E. coli rfaD gene, which encodes ADP-L-glycero-D-mannoheptose epimerase. This enzyme is involved in the biosynthesis of the lipopolysaccharide precursor ADP-L-glycero-D-mannoheptose. A site-directed frameshift mutation i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2321-2327.1995

    authors: Drazek ES,Stein DC,Deal CD

    更新日期:1995-05-01 00:00:00

  • Dinitrogenase reductase-activating glycohydrolase can be released from chromatophores of Rhodospirillum rubrum by treatment with MgGDP.

    abstract::Dinitrogenase reductase-activating glycohydrolase (DRAG), involved in the regulation of nitrogenase activity in Rhodospirillum rubrum, is associated with chromatophore membranes in cell extracts. We show that DRAG can be specifically released by treatment with MgGDP; other nucleotides studied had no effect. The DRAG a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7872-7874.1997

    authors: Norén A,Nordlund S

    更新日期:1997-12-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

    abstract::Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1266-1276.1964

    authors: KONDO E,MITSUHASHI S

    更新日期:1964-11-01 00:00:00

  • Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities.

    abstract::The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.10.3551-3571.2006

    authors: Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

    更新日期:2006-05-01 00:00:00

  • Construction and characterization of an Escherichia coli strain with a uncI mutation.

    abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.820-825.1984

    authors: Gay NJ

    更新日期:1984-06-01 00:00:00

  • Genome sequence of the bacteriocin-producing strain Lactococcus garvieae DCC43.

    abstract::This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01864-12

    authors: Gabrielsen C,Brede DA,Hernández PE,Nes IF,Diep DB

    更新日期:2012-12-01 00:00:00

  • Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein.

    abstract::The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4064-4069.1992

    authors: Choi SH,Greenberg EP

    更新日期:1992-06-01 00:00:00

  • Nitrate-dependent regulation of acetate biosynthesis and nitrate respiration by Clostridium thermoaceticum.

    abstract::Nitrate has been shown to shunt the electron flow in Clostridium thermoaceticum from CO2 to nitrate, but it did not influence the levels of enzymes involved in the Wood-Ljungdahl pathway (J. M. Fröstl, C. Seifritz, and H. L. Drake, J. Bacteriol. 178:4597-4603, 1996). Here we show that under some growth conditions, nit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.5.1489-1495.1999

    authors: Arendsen AF,Soliman MQ,Ragsdale SW

    更新日期:1999-03-01 00:00:00

  • Site-specific serine incorporation by Lif and Epr into positions 3 and 5 of the Staphylococcal peptidoglycan interpeptide bridge.

    abstract::The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.9.2635-2638.2000

    authors: Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

    更新日期:2000-05-01 00:00:00

  • Genetic evidence of distinct physiological regulation mechanisms in the sigma(54) Pu promoter of Pseudomonas putida.

    abstract::The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.956-960.2000

    authors: Cases I,de Lorenzo V

    更新日期:2000-02-01 00:00:00