Abstract:
:Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant was analyzed. Under aerobic conditions, the specific growth rate during exponential phase (mu(e)) of the A. brasilense cytN mutant was comparable to the wild-type specific growth rate (m(e) of approximately 0.2 h-1). In microaerobic NH4+-supplemented conditions, the low respiration of the A. brasilense cytN mutant affected its specific growth rate (mu(e) of approximately 0.02 h-1) compared to the wild-type specific growth rate (mu(e) of approximately 0.2 h-1). Under nitrogen-fixing conditions, both the growth rates and respiration of the wild type were significantly diminished in comparison to those under NH4+-supplemented conditions. Differences in growth rates and respiration between the wild type and the A. brasilense cytN mutant were less pronounced under these nitrogen-fixing conditions (mu(e) of approximately 0.03 h-1 for the wild type and 0.02 h-1 for the A. brasilense cytN mutant). The nitrogen-fixing capacity of the A. brasilense cytN mutant was still approximately 80% of that determined for the wild-type strain. This leads to the conclusion that the A. brasilense cytochrome cbb3 oxidase is required under microaerobic conditions, when a high respiration rate is needed, but that under nitrogen-fixing conditions the respiration rate does not seem to be a growth-limiting factor.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Marchal K,Sun J,Keijers V,Haaker H,Vanderleyden Jdoi
10.1128/JB.180.21.5689-5696.1998subject
Has Abstractpub_date
1998-11-01 00:00:00pages
5689-96issue
21eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00466-15
更新日期:2015-11-01 00:00:00
abstract::The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.01084-08
更新日期:2009-01-01 00:00:00
abstract::Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attract Escherichia coli bacteria. The most effective attractants, i.e., those that have thresholds near 10(-5) M or below, are N-acetyl-d-glucosamine, 6-deoxy-d-glucose, d-fructose, d-fucose, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.824-847.1973
更新日期:1973-09-01 00:00:00
abstract::A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01279-12
更新日期:2012-10-01 00:00:00
abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.107-118.1998
更新日期:1998-01-01 00:00:00
abstract::Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.127-135.1971
更新日期:1971-01-01 00:00:00
abstract::A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6177-6182.2000
更新日期:2000-11-01 00:00:00
abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1425-1430.1968
更新日期:1968-04-01 00:00:00
abstract::Streptomyces linear plasmids and linear chromosomes can replicate also in a circular form when their telomeres are deleted. The 17-kb linear plasmid pSLA2 has been a useful model in studies of such replicons. Here we report that the minimal origin initiating replication of pSLA2-derived plasmids as circular molecules ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6575-6582.2003
更新日期:2003-11-01 00:00:00
abstract::Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.544-553.1968
更新日期:1968-08-01 00:00:00
abstract::Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1635-1637.1966
更新日期:1966-04-01 00:00:00
abstract::Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.620-624.1979
更新日期:1979-08-01 00:00:00
abstract::Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-05
更新日期:2006-07-01 00:00:00
abstract::The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5539-5545.1991
更新日期:1991-09-01 00:00:00
abstract::Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.755-760.1983
更新日期:1983-08-01 00:00:00
abstract::In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5417-5421.1996
更新日期:1996-09-01 00:00:00
abstract::A protease-activated antimicrobial peptide (PAMP) and its inactive precursor were purified from the culture supernatant of Propionibacterium jensenii LMG 3032 and characterized at the molecular level. PAMP is a 64-amino-acid cationic peptide of 6,383 Da with physicochemical features similar to those of bacteriocins fr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3649-3656.2002
更新日期:2002-07-01 00:00:00
abstract::The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.9.2416-2421.2000
更新日期:2000-05-01 00:00:00
abstract::Expression of the hurIR bhuRSTUV heme utilization locus in Bordetella bronchiseptica is coordinately controlled by the global iron-dependent regulator Fur and the extracytoplasmic function sigma factor HurI. Activation of HurI requires transduction of a heme-dependent signal via HurI, HurR, and BhuR, a three-component...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00629-07
更新日期:2007-09-01 00:00:00
abstract::Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.846-852.1979
更新日期:1979-06-01 00:00:00
abstract::Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.108-121.1976
更新日期:1976-04-01 00:00:00
abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8256-8266.2005
更新日期:2005-12-01 00:00:00
abstract::The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.12.3960-3969.2004
更新日期:2004-06-01 00:00:00
abstract::The mutations of eight chemotaxis-deficient strains of Salmonella typhimurium, including five new mutants in strain LT2, were mapped by P22 transduction in relation to various fla mot deletions in S. abortus-equi. Seven recessive che mutations mapped between motB and flaC: three, all nontumbling, the che region I, adj...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.3.754-765.1976
更新日期:1976-12-01 00:00:00
abstract::Gauntt, Charles J. (The University of Texas, Austin), and Royce Z. Lockart, Jr. Inhibition of Mengo virus by interferon. J. Bacteriol. 91:176-182. 1966.-The inhibition of Mengo virus replication in L cells resulting from interferon was studied quantitatively. Interferon was titrated on L cells with Western equine ence...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.176-182.1966
更新日期:1966-01-01 00:00:00
abstract::Dobrogosz, Walter J. (The Pennsylvania State University, University Park) and Robert W. Stone. Oxidative metabolism in Pediococcus pentosaceus. II. Factors controlling the formation of oxidative activities. J. Bacteriol. 84:724-729. 1962.-Studies were conducted on some factors which regulate the formation of oxidative...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.724-729.1962
更新日期:1962-10-01 00:00:00
abstract::Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01234-12
更新日期:2013-01-01 00:00:00
abstract::Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01324-09
更新日期:2010-04-01 00:00:00
abstract::Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00323-12
更新日期:2012-05-01 00:00:00
abstract::Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.4.910-919.1964
更新日期:1964-04-01 00:00:00