Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

journal_title：Journal of bacteriology

authors： Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

更新日期：1984-10-01 00:00:00

abstract：:Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that enc...

journal_title：Journal of bacteriology

authors： Gaidelyte A,Jaatinen ST,Daugelavicius R,Bamford JK,Bamford DH

更新日期：2005-05-01 00:00:00

abstract：:In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...

journal_title：Journal of bacteriology

authors： Benson S,Shapiro J

更新日期：1975-08-01 00:00:00

abstract：:Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

journal_title：Journal of bacteriology

authors： Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

更新日期：2015-01-01 00:00:00

abstract：:In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...

journal_title：Journal of bacteriology

authors： Van Brussel AA,Zaat SA,Cremers HC,Wijffelman CA,Pees E,Tak T,Lugtenberg BJ

更新日期：1986-02-01 00:00:00

abstract：:The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...

journal_title：Journal of bacteriology

authors： Terrak M,Nguyen-Distèche M

更新日期：2006-04-01 00:00:00

abstract：:When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E,...

journal_title：Journal of bacteriology

authors： Yoshida T,Katagiri K

更新日期：1967-04-01 00:00:00

abstract：:Flagellate bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium typically express 5 to 12 flagellar filaments over their cell surface that rotate in clockwise (CW) and counterclockwise directions. These bacteria modulate their swimming direction towards favorable environments by biasing the di...

journal_title：Journal of bacteriology

authors： Morehouse KA,Goodfellow IG,Sockett RE

更新日期：2005-03-01 00:00:00

abstract：:Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...

journal_title：Journal of bacteriology

authors： Booth IR,Blount P

更新日期：2012-09-01 00:00:00

abstract：:A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

journal_title：Journal of bacteriology

authors： Musser JM,Bemis DA,Ishikawa H,Selander RK

更新日期：1987-06-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:In Anacystis nidulans, upon infection with cyanophage AS-1, after a lag period of 1 h the level of deoxyribonuclease (DNase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. In contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, ...

journal_title：Journal of bacteriology

authors： Udvardy J,Sivok B,Borbely G,Farkas GL

更新日期：1976-05-01 00:00:00

abstract：:The bacterium Myxococcus xanthus alternates between two colony types distinguished by colony morphology and pigmentation. Because the two phases are interconvertible, this phenomenon has been termed phase variation. In one phase, the colonies are bright yellow, rough, and swarming. In the alternate phase, the colonies...

journal_title：Journal of bacteriology

authors： Laue BE,Gill RE

更新日期：1994-09-01 00:00:00

abstract：:The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

journal_title：Journal of bacteriology

authors： Nahlik MS,Fleming TP,McIntosh MA

更新日期：1987-09-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:It was previously shown that the Rhodobacter capsulatus NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Escherichia coli RNA polymerase at the nifA1 promoter. We have tested the hypothesis that this activity is due to the high G+C content of the -10 sequence. A compara...

journal_title：Journal of bacteriology

authors： Richard CL,Tandon A,Kranz RG

更新日期：2004-02-01 00:00:00

abstract：:Pseudomonas putida KT2440 is a soil microorganism that attaches to seeds and efficiently colonizes the plant's rhizosphere. Lysine is one of the major compounds in root exudates, and P. putida KT2440 uses this amino acid as a source of carbon, nitrogen, and energy. Lysine is channeled to delta-aminovaleric acid and th...

journal_title：Journal of bacteriology

authors： Revelles O,Espinosa-Urgel M,Molin S,Ramos JL

更新日期：2004-06-01 00:00:00

abstract：:Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

journal_title：Journal of bacteriology

authors： Cybulska J,Jeljaszewicz J

更新日期：1966-03-01 00:00:00

abstract：:A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all ...

journal_title：Journal of bacteriology

authors： Plazinski J,Dart PJ,Rolfe BG

更新日期：1983-09-01 00:00:00

abstract：:The colicinogenic B factor, transferred from Escherichia coli strain K77 (and termed ColB2-K77 or ColB2) to an E. coli K12 F(-) strain, is capable of promoting its own transfer to other K12 F(-) strains at a low rate (from LFC cultures) which can be increased under special conditions (HFC cultures). LFC cultures of K1...

journal_title：Journal of bacteriology

authors： Hausmann C,Clowes RC

更新日期：1971-09-01 00:00:00

abstract：:Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

journal_title：Journal of bacteriology

authors： Allen NE,MacQuillan AM

更新日期：1969-03-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA) reassociation analyses were employed to determine the molecular relationships between recombinable nocardiae. Analysis of the compatibility system of Nocardia erythropolis Mat-Ce and Mat-cE mating strains demonstrated the existence of extensive homology under both exacting and nonexacting c...

journal_title：Journal of bacteriology

authors： Clark JE,Brownell GH

更新日期：1972-02-01 00:00:00

abstract：:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...

journal_title：Journal of bacteriology

authors： Van Alstyne D,Simon MI

更新日期：1971-12-01 00:00:00

abstract：:Hydroxylamine mutagenesis was used to alter the tar gene that encodes the transmembrane Tar protein required for chemotaxis. Mutants defective in chemotaxis were selected, and the mutation was characterized by DNA sequencing. Two classes of mutations were found: nonsense and missense. The nonsense mutations were distr...

journal_title：Journal of bacteriology

authors： Mutoh N,Oosawa K,Simon MI

更新日期：1986-09-01 00:00:00

abstract：:Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...

journal_title：Journal of bacteriology

authors： Wróblewski H

更新日期：1979-11-01 00:00:00

abstract：:Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...

journal_title：Journal of bacteriology

authors： FREEMAN BM,CHUBB LG

更新日期：1964-07-01 00:00:00

abstract：:Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activiti...

journal_title：Journal of bacteriology

authors： Habe H,Chung JS,Kato H,Ayabe Y,Kasuga K,Yoshida T,Nojiri H,Yamane H,Omori T

更新日期：2004-09-01 00:00:00

abstract：:Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...

journal_title：Journal of bacteriology

authors： Cotter DA,Raper KB

更新日期：1968-11-01 00:00:00