Characterization of Escherichia coli chemotaxis receptor mutants with null phenotypes.

abstract：:Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

journal_title：Journal of bacteriology

authors： Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

更新日期：2002-05-01 00:00:00

abstract：:The gene GK3045 (741 bp) from Geobacillus kaustophilus HTA426 was cloned, sequenced, and overexpressed into Escherichia coli Rosetta (DE3). The deduced protein was a 30-kDa monomeric esterase with high homology to carboxylesterases from Geobacillus thermoleovorans NY (99% identity) and Geobacillus stearothermophilus (...

journal_title：Journal of bacteriology

authors： Montoro-García S,Martínez-Martínez I,Navarro-Fernández J,Takami H,García-Carmona F,Sánchez-Ferrer A

更新日期：2009-05-01 00:00:00

abstract：:A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

journal_title：Journal of bacteriology

authors： Baum JA

更新日期：1994-05-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

journal_title：Journal of bacteriology

authors： Camakaris J,Pittard J

更新日期：1971-08-01 00:00:00

abstract：:DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

journal_title：Journal of bacteriology

authors： Washburn BK,Kushner SR

更新日期：1991-04-01 00:00:00

abstract：:The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...

journal_title：Journal of bacteriology

authors： Errey JC,Blanchard JS

更新日期：2005-05-01 00:00:00

abstract：:Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produ...

journal_title：Journal of bacteriology

authors： Straight PD,Willey JM,Kolter R

更新日期：2006-07-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:We have carefully characterized and reexamined the motility and phototactic responses of Synechocystis sp. adenylyl cyclase (Cya1) and catabolite activator protein (SYCRP1) mutants to different light regimens, glucose, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and cyclic AMP. We find that contrary to earlier reports, c...

journal_title：Journal of bacteriology

authors： Bhaya D,Nakasugi K,Fazeli F,Burriesci MS

更新日期：2006-10-01 00:00:00

abstract：:The isopropylmalate isomerase in Salmonella typhimurium is the second enzyme specific for leucine biosynthesis. It is a complex enzyme composed of two subunits which are coded for by two genes of the leucine operon, leuC and leuD. The two polypeptides have been shown to copurify through successive ammonium sulfate fra...

journal_title：Journal of bacteriology

authors： Fultz PN,Kemper J

更新日期：1981-10-01 00:00:00

abstract：:Small subunit rDNA sequences were determined for 20 species of the genera Acetogenium, Clostridium, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, and Thermobacteroides, 3 non-validly described species, and 5 isolates of anaerobic thermophilic bacteria, providing a basis for a phylogenetic analysis of th...

journal_title：Journal of bacteriology

authors： Rainey FA,Ward NL,Morgan HW,Toalster R,Stackebrandt E

更新日期：1993-08-01 00:00:00

abstract：:The oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such as Porphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon u...

journal_title：Journal of bacteriology

authors： Rocco CJ,Bakaletz LO,Goodman SD

更新日期：2018-05-09 00:00:00

abstract：:TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

journal_title：Journal of bacteriology

authors： Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

更新日期：1997-12-01 00:00:00

abstract：:Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...

journal_title：Journal of bacteriology

authors： Whiteway J,Koziarz P,Veall J,Sandhu N,Kumar P,Hoecher B,Lambert IB

更新日期：1998-11-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...

journal_title：Journal of bacteriology

authors： Seidler RJ,Starr MP,Mandel M

更新日期：1969-11-01 00:00:00

abstract：:To circumvent the permeability barrier of its outer membrane, Pseudomonas aeruginosa has evolved a series of specific porins. These channels have binding sites for related classes of molecules that facilitate uptake under nutrient-limited conditions. Here, we report on the identification of a 19-member family of porin...

journal_title：Journal of bacteriology

authors： Tamber S,Ochs MM,Hancock RE

更新日期：2006-01-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...

journal_title：Journal of bacteriology

authors： Nataro JP,Yikang D,Yingkang D,Walker K

更新日期：1994-08-01 00:00:00

abstract：:The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939(T) was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal ...

journal_title：Journal of bacteriology

authors： Heuner K,Choi BK,Schade R,Moter A,Otto A,Göbel UB

更新日期：1999-02-01 00:00:00

abstract：:An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

journal_title：Journal of bacteriology

authors： Ma Z,Richard H,Tucker DL,Conway T,Foster JW

更新日期：2002-12-01 00:00:00

abstract：:A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chem...

journal_title：Journal of bacteriology

authors： Ordal GW,Parker HM,Kirby JR

更新日期：1985-11-01 00:00:00

abstract：:The initial reactions involved in the catabolism of fructose in Pseudomonas aeruginosa include the participation of a phosphoenolpyruvate:fructose 1-phosphotransferase system (F-PTS). Fractionation of crude extracts of fructose-grown cells revealed that both membrane-associated and soluble components were essential fo...

journal_title：Journal of bacteriology

authors： Durham DR,Phibbs PV Jr

更新日期：1982-02-01 00:00:00

abstract：:The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

journal_title：Journal of bacteriology

authors： Tanous C,Chambellon E,Sepulchre AM,Yvon M

更新日期：2005-07-01 00:00:00

abstract：:Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

journal_title：Journal of bacteriology

authors： Gelsinger DR,DiRuggiero J

更新日期：2018-04-09 00:00:00

abstract：:Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

journal_title：Journal of bacteriology

authors： da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

更新日期：2011-06-01 00:00:00

abstract：:Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...

journal_title：Journal of bacteriology

authors： Shane B,Bognar AL,Goldfarb RD,LeBowitz JH

更新日期：1983-01-01 00:00:00

abstract：:Cultures of recombination-deficient strains of Escherichia coli are composed of three classes of cells: (i) viable cells, which can undergo 20 or more generations, (ii) residually dividing cells, which can undergo fewer than 20 generations (probably an average of fewer than 6), and (iii) nondividing cells, which are i...

journal_title：Journal of bacteriology

authors： Capaldo FN,Ramsey G,Barbour SD

更新日期：1974-04-01 00:00:00

abstract：:The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

journal_title：Journal of bacteriology

authors： Umbreit JN,Strominger JL

更新日期：1972-12-01 00:00:00

abstract：:The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

journal_title：Journal of bacteriology

authors： Austin SJ

更新日期：1984-05-01 00:00:00