Abstract:
:The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identical within the limits of experimental error. Most cultures of Bdellovibrio bacteriovorus, including the holotype culture, have 50.4 +/- 0.9 moles% GC in their DNA; two bdellovibrio isolates of presently uncertain nomenclatural status contain DNA of about 43% GC. Optical melting profiles of all the DNA from all of these organisms are particularly steep, indicating little compositional heterogeneity. Chromatography of acid hydrolysates of Bdellovibrio nucleic acids reveal no unusual components. The DNA content per cell of one H-I derivative is about one-third the amount per Escherichia coli cell growing at a comparable rate.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Seidler RJ,Starr MP,Mandel Mdoi
10.1128/JB.100.2.786-790.1969subject
Has Abstractpub_date
1969-11-01 00:00:00pages
786-90issue
2eissn
0021-9193issn
1098-5530journal_volume
100pub_type
杂志文章abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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