Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100.

abstract：:Partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 C for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. The molecular weight of the inactive glucose dehydrogenase in the heated spores is about ...

journal_title：Journal of bacteriology

authors： Hachisuka Y,Tochikubo K

更新日期：1971-08-01 00:00:00

abstract：:Anaerobic microbial toluene catabolism is initiated by addition of fumarate to the methyl group of toluene, yielding (R)-benzylsuccinate as first intermediate, which is further metabolized via beta-oxidation to benzoyl-coenzyme A (CoA) and succinyl-CoA. A specific succinyl-CoA:(R)-benzylsuccinate CoA-transferase activ...

journal_title：Journal of bacteriology

authors： Leutwein C,Heider J

更新日期：2001-07-01 00:00:00

abstract：:CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more...

journal_title：Journal of bacteriology

authors： Ohtake H,Cervantes C,Silver S

更新日期：1987-08-01 00:00:00

abstract：:The Enterococcus faecalis plasmid pAD1 undergoes conjugative transfer in response to cAD1, a peptide sex pheromone emitted by potential bacterial recipients. Regulation of pAD1 transfer involves a number of plasmid-encoded determinants:iad, which determines a peptide-competitive inhibitor iAD1; signal sensing and tran...

journal_title：Journal of bacteriology

authors： Pontius LT,Clewell DB

更新日期：1992-05-01 00:00:00

abstract：:Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-di...

journal_title：Journal of bacteriology

authors： Sariaslani FS,Sudmeier JL,Focht DD

更新日期：1982-10-01 00:00:00

abstract：:The tar locus of Escherichia coli specifies one of the major species of methyl-accepting proteins involved in the chemotactic behavior of this organism. The physical and genetic organization of the tar region was investigated with a series of specialized lambda transducing phages and plasmid clones. The tar gene was m...

journal_title：Journal of bacteriology

authors： Slocum MK,Parkinson JS

更新日期：1983-08-01 00:00:00

abstract：:Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

journal_title：Journal of bacteriology

authors： MORELLO JA,DIGENIO TA,BAKER EE

更新日期：1965-06-01 00:00:00

abstract：:The trehalose content in Saccharomyces cerevisiae can be significantly manipulated by including trehalose at an appropriate level in the growth medium. Its uptake is largely dependent on the expression of AGT1, which encodes an alpha-glucoside transporter. The trehalose found in a tps1 mutant of trehalose synthase may...

journal_title：Journal of bacteriology

authors： Plourde-Owobi L,Durner S,Parrou JL,Wieczorke R,Goma G,François J

更新日期：1999-06-01 00:00:00

abstract：:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

journal_title：Journal of bacteriology

authors： Fouts KE,Barbour SD

更新日期：1982-01-01 00:00:00

abstract：:Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...

journal_title：Journal of bacteriology

authors： Reich PR,Somerson NL,Rose JA,Weissman SM

更新日期：1966-01-01 00:00:00

abstract：:Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretio...

journal_title：Journal of bacteriology

authors： Hodges LD,Lee LY,McNett H,Gelvin SB,Ream W

更新日期：2009-01-01 00:00:00

abstract：:A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...

journal_title：Journal of bacteriology

authors： Weiss RL

更新日期：1976-11-01 00:00:00

abstract：:The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

journal_title：Journal of bacteriology

authors： Huang KJ,Schieberl JL,Igo MM

更新日期：1994-03-01 00:00:00

abstract：:Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...

journal_title：Journal of bacteriology

authors： Sarubbi FA Jr,Sparling PF,Blackman E,Lewis E

更新日期：1975-11-01 00:00:00

abstract：:A conditionally germination-defective mutant of Neurospora crassa has been found to be partially curable by ferricrocin and other siderophores. The mutant conidia rapidly lose their membrane-bound siderophores when suspended in buffer or growth media. Germination is consequently delayed unless large numbers of conidia...

journal_title：Journal of bacteriology

authors： Charlang G,Williams NP

更新日期：1977-12-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...

journal_title：Journal of bacteriology

authors： Postlethwait P,Sundstrom P

更新日期：1995-04-01 00:00:00

abstract：:With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two prot...

journal_title：Journal of bacteriology

authors： Putzer H,Brakhage AA,Grunberg-Manago M

更新日期：1990-08-01 00:00:00

abstract：:The peptidoglycan layer surrounding the photosynthetic organelles (cyanelles) of the protist Cyanophora paradoxa is thought to be a relic of their cyanobacterial ancestors. The separation of muropeptides by gel filtration and reverse-phase high-performance liquid chromatography revealed four different muropeptide mono...

journal_title：Journal of bacteriology

authors： Pfanzagl B,Zenker A,Pittenauer E,Allmaier G,Martinez-Torrecuadrada J,Schmid ER,De Pedro MA,Löffelhardt W

更新日期：1996-01-01 00:00:00

abstract：:The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

journal_title：Journal of bacteriology

authors： Ou JT,Reim R

更新日期：1976-10-01 00:00:00

abstract：:Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

journal_title：Journal of bacteriology

authors： Schulman M,Valentino D

更新日期：1976-10-01 00:00:00

abstract：:The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

journal_title：Journal of bacteriology

authors： Leventhal JM,Chambliss GH

更新日期：1982-12-01 00:00:00

abstract：:Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...

journal_title：Journal of bacteriology

authors： Ebisu S,Kato K,Kotani S,Misaki A

更新日期：1975-12-01 00:00:00

abstract：:The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

journal_title：Journal of bacteriology

authors： Mori H,Shimokawa N,Satoh Y,Ito K

更新日期：2004-06-01 00:00:00

abstract：:An inorganic pyrophosphatase was purified over 600-fold to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme is a tetramer of Mr = 84,000, has a sedimentation coefficient of 5.8S, a Stokes radius of 3.5 nm, and an isoelectric point of 5.7. Like the enzyme of Escherichia coli, the pyrophosphatase ...

journal_title：Journal of bacteriology

authors： Verhoeven JA,Schenck KM,Meyer RR,Trela JM

更新日期：1986-10-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:Cultures of isocitrate dehydrogenase-deficient (icd) mutants were overgrown by double mutants (icd glt) lacking citrate synthase activity also. The icd mutants grew more slowly than wild-type cells or the double mutants because they accumulated an inhibitory metabolite (possibly citrate). Intracellular citrate levels ...

journal_title：Journal of bacteriology

authors： Lakshmi TM,Helling RB

更新日期：1976-07-01 00:00:00

abstract：:Adsorption of phages T4, T5, and BF23 to previously starved Escherichia coli cells triggered the immediate release of respiratory control. A similar stimulation of respiration was induced after T4 ghost attachment, showing that this process was independent of the mechanism of DNA injection. Rather, this change in the ...

journal_title：Journal of bacteriology

authors： Letellier L,Labedan B

更新日期：1985-01-01 00:00:00

abstract：:Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (agr locus) as well as multiple virulence-associated genes in Staphylococcus aureus. Gene expression was examined during growth of S. aureus in vitro in standard laboratory medium and rabbit serum and in vivo in subcuta...

journal_title：Journal of bacteriology

authors： Yarwood JM,McCormick JK,Paustian ML,Kapur V,Schlievert PM

更新日期：2002-02-01 00:00:00

abstract：:Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

journal_title：Journal of bacteriology

authors： Popham DL,Meador-Parton J,Costello CE,Setlow P

更新日期：1999-10-01 00:00:00