Synthesis of acid-soluble spore proteins by Bacillus subtilis.

abstract：:Rod-shaped bacteria grow by a repetitive cycle of elongation followed by division, and the mechanisms responsible for these two processes have been studied for decades. However, little is known about what happens during the transition between the two activities. At least one event occurs after elongation ends and befo...

journal_title：Journal of bacteriology

authors： Potluri LP,Kannan S,Young KD

更新日期：2012-10-01 00:00:00

abstract：:Glucose kinase is required for glucose repression of several catabolite-controlled genes in Streptomyces coelicolor and certainly plays an important role in glucose repression in these organisms. We report here that glucose kinase null mutants of S. coelicolor retain transcriptional regulation of the chitinase 63 gene...

journal_title：Journal of bacteriology

authors： Ingram C,Westpheling J

更新日期：1995-06-01 00:00:00

abstract：:We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

journal_title：Journal of bacteriology

authors： McDonough KA,Hare JM

更新日期：1997-03-01 00:00:00

abstract：:Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manifested only when the medium contained exogenous leucine, suggesting that mutant ce...

journal_title：Journal of bacteriology

authors： McEwen J,Silverman P

更新日期：1980-10-01 00:00:00

abstract：:Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...

journal_title：Journal of bacteriology

authors： Vitković L,Cheung HY,Freese E

更新日期：1984-01-01 00:00:00

abstract：:Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacteria] group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxa...

journal_title：Journal of bacteriology

authors： Mills DA,McKay LL,Dunny GM

更新日期：1996-06-01 00:00:00

abstract：:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

journal_title：Journal of bacteriology

authors： Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

更新日期：1992-01-01 00:00:00

abstract：:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

journal_title：Journal of bacteriology

authors： Warner JR,Morgan SA,Shulman RW

更新日期：1976-03-01 00:00:00

abstract：:The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...

journal_title：Journal of bacteriology

authors： Richter G,Fischer M,Krieger C,Eberhardt S,Lüttgen H,Gerstenschläger I,Bacher A

更新日期：1997-03-01 00:00:00

abstract：:We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...

journal_title：Journal of bacteriology

authors： Calvin NM,Hanawalt PC

更新日期：1988-06-01 00:00:00

abstract：:We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

journal_title：Journal of bacteriology

authors： Davis LC,Wang YL

更新日期：1980-03-01 00:00:00

abstract：:We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...

journal_title：Journal of bacteriology

authors： Gimenez R,Nuñez MF,Badia J,Aguilar J,Baldoma L

更新日期：2003-11-01 00:00:00

abstract：:The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

journal_title：Journal of bacteriology

authors： McFarland KA,Lucchini S,Hinton JC,Dorman CJ

更新日期：2008-01-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:Using yeast two-hybrid screens, we have defined an interaction group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili. The six proteins are TraH, TraF, TraW, TraU, TrbI, and TrbB. Except for TrbI, these proteins were all identified as hallmarks of F-like type IV secretion sys...

journal_title：Journal of bacteriology

authors： Harris RL,Silverman PM

更新日期：2004-08-01 00:00:00

abstract：:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

journal_title：Journal of bacteriology

authors： Irazabal N,Marín I,Amils R

更新日期：1997-03-01 00:00:00

abstract：:The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...

journal_title：Journal of bacteriology

authors： Pudlik AM,Lolkema JS

更新日期：2012-07-01 00:00:00

abstract：:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

journal_title：Journal of bacteriology

authors： Severance MM,LaRock PA

更新日期：1973-12-01 00:00:00

abstract：:Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...

journal_title：Journal of bacteriology

authors： Weinberg MV,Jenney FE Jr,Cui X,Adams MW

更新日期：2004-12-01 00:00:00

abstract：:Fatty acid desaturation catalyzed by fatty acid desaturases requires molecular oxygen (O(2)). Saccharomyces cerevisiae cells derepress expression of OLE1 encoding Delta9 fatty acid desaturase under hypoxic conditions to allow more-efficient use of limited O(2). It has been proposed that aerobic conditions lead to repr...

journal_title：Journal of bacteriology

authors： Nakagawa Y,Sugioka S,Kaneko Y,Harashima S

更新日期：2001-01-01 00:00:00

abstract：:The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...

journal_title：Journal of bacteriology

authors： Prüss BM,Campbell JW,Van Dyk TK,Zhu C,Kogan Y,Matsumura P

更新日期：2003-01-01 00:00:00

abstract：:The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

journal_title：Journal of bacteriology

authors： Moralejo P,Egan SM,Hidalgo E,Aguilar J

更新日期：1993-09-01 00:00:00

abstract：:The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented....

journal_title：Journal of bacteriology

authors： Mendelson NH,Thwaites JJ,Kessler JO,Li C

更新日期：1995-12-01 00:00:00

abstract：:Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...

journal_title：Journal of bacteriology

authors： Gonzalez F,Helm RF,Broadway KM,Scharf BE

更新日期：2018-09-10 00:00:00

abstract：:The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...

journal_title：Journal of bacteriology

authors： Brown JW,Haas ES,James BD,Hunt DA,Liu JS,Pace NR

更新日期：1991-06-01 00:00:00

abstract：:The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

journal_title：Journal of bacteriology

authors： Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

更新日期：2006-05-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98 degrees C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a ...

journal_title：Journal of bacteriology

authors： Horn C,Paulmann B,Kerlen G,Junker N,Huber H

更新日期：1999-08-01 00:00:00

abstract：:The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

journal_title：Journal of bacteriology

authors： Wilson RL,Urbanowski ML,Stauffer GV

更新日期：1995-09-01 00:00:00

abstract：:Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

journal_title：Journal of bacteriology

authors： Quay SC,Oxender DL

更新日期：1977-06-01 00:00:00