Abstract:
:The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented. Subsequent folds that produced multistrandedness were also examined. The rate of loop rotation during winding up was measured for 26 loops from 16 clones. In most cases, the first loop formed turned at a lower rate than those produced by the following cycles of folding. The sequence of folding topologies differed in FJ7 and RHX strains and in left- versus right-handed structures. Right-handed FJ7 routinely gave rise to four-stranded helices at the second fold, whereas left-handed FJ7 and both left-handed and right-handed forms of RHX made structures with predominantly two double-stranded helical regions. Left-handed RHX structures frequently produced second folds within the initial loop itself, resulting in T- or Y-shaped fibers. Sixteen cases in which the initial touch of a filament to itself produced a loop that snapped open before it could wind up into a double-strand fiber were found. The snap motions were used to obtain estimates of the forces generated by helical growth of single filaments and to investigate theoretical models involving the material properties of cell filaments. In general, the mechanical behavior of growing single-cell filaments and fibers consisting of two-, three-, or four-strand helices was similar to that described for larger, mature, multifilament macrofibers. The behavior of multicellular macrofibers can be understood, therefore, in terms of individual cell growth.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mendelson NH,Thwaites JJ,Kessler JO,Li Cdoi
10.1128/jb.177.24.7060-7069.1995subject
Has Abstractpub_date
1995-12-01 00:00:00pages
7060-9issue
24eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.407-409.1985
更新日期:1985-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1112-1119.1982
更新日期:1982-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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doi:10.1128/JB.91.1.153-160.1966
更新日期:1966-01-01 00:00:00
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更新日期:1992-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.147.3.1049-1053.1981
更新日期:1981-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01429-07
更新日期:2008-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.951-959.1977
更新日期:1977-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.176.24.7558-7565.1994
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更新日期:1978-06-01 00:00:00
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更新日期:1984-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1979-12-01 00:00:00
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7210-7221.1995
更新日期:1995-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.892-900.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01289-08
更新日期:2009-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.643-649.1989
更新日期:1989-02-01 00:00:00
abstract::Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00275-16
更新日期:2016-07-28 00:00:00