Abstract:
:Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was identified in the virulence (vir) region of the Ti plasmid. This fragment contains the virG locus, as well as the 3' end of the virB operon. A clone of this fragment conferred the supervirulence phenotype on A348, a nonsupervirulent strain. The increased virulence was correlated with an increased expression of vir genes, which could be achieved by introducing an extra copy of the transcriptional activator virG or the supervirulence region for maximum virulence. The virulence of the supervirulent strain A281 could be increased even further if the entire virB operon was added in addition to the virG operon. A plasmid, pToK47, containing virB and virG increased the virulence of all A. tumefaciens strains into which the plasmid was introduced. These data suggest that a highly virulent binary vector system can be constructed which might prove especially useful in the transformation of certain higher plants.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Jin SG,Komari T,Gordon MP,Nester EWdoi
10.1128/jb.169.10.4417-4425.1987subject
Has Abstractpub_date
1987-10-01 00:00:00pages
4417-25issue
10eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::The kinetic parameters of the release of Ca(2+)-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06452-11
更新日期:2012-02-01 00:00:00
abstract::Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01476-12
更新日期:2013-01-01 00:00:00
abstract::DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.4851-4856.1995
更新日期:1995-09-01 00:00:00
abstract::Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.870-875.1966
更新日期:1966-02-01 00:00:00
abstract::Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02141-12
更新日期:2013-04-01 00:00:00
abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.12.3031-3038.1998
更新日期:1998-06-01 00:00:00
abstract::In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization to develop a techniq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5641-5647.1987
更新日期:1987-12-01 00:00:00
abstract::We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.21.6448-6455.2003
更新日期:2003-11-01 00:00:00
abstract::A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.4127-4131.1990
更新日期:1990-07-01 00:00:00
abstract::The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00062-18
更新日期:2018-06-25 00:00:00
abstract::The direct binding of Streptococcus mitis to human platelets is mediated in part by two proteins (PblA and PblB) encoded by a lysogenic bacteriophage (SM1). Since SM1 is the first prophage of S. mitis that has been identified and because of the possible role of these phage-encoded proteins in virulence, we sought to c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6968-6975.2003
更新日期:2003-12-01 00:00:00
abstract::The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00267-17
更新日期:2017-08-08 00:00:00
abstract::Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.402-413.1978
更新日期:1978-10-01 00:00:00
abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.2.284-290.1994
更新日期:1994-01-01 00:00:00
abstract::Deoxycytosine methylase (Dcm) enzyme activity causes mutagenesis in vitro either directly by enzyme-induced deamination of cytosine to uracil in the absence of the methyl donor, S-adenosylmethionine (SAM), or indirectly through spontaneous deamination of [5-methyl]cytosine to thymine. Using a Lac reversion assay, we i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.921-927.2001
更新日期:2001-02-01 00:00:00
abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4047-4054.1988
更新日期:1988-09-01 00:00:00
abstract::The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.626-633.1971
更新日期:1971-05-01 00:00:00
abstract::A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5968-5979.1990
更新日期:1990-10-01 00:00:00
abstract::The Haemophilus cryptic genospecies is an important cause of maternal genital tract and neonatal systemic infections and initiates infection by colonizing the genital or respiratory epithelium. In recent work, we identified a unique Haemophilus cryptic genospecies protein called Cha, which mediates efficient adherence...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00933-10
更新日期:2011-01-01 00:00:00
abstract::The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a pote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.5.1409-1414.1999
更新日期:1999-03-01 00:00:00
abstract::Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.347-349.1980
更新日期:1980-04-01 00:00:00
abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.5019-5022.2005
更新日期:2005-07-01 00:00:00
abstract::A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.238-246.1980
更新日期:1980-10-01 00:00:00
abstract::The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01347-08
更新日期:2009-01-01 00:00:00
abstract::The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. (13)C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45 degrees C revealed the presence of hydroxyectoine, ectoine, glutam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00136-06
更新日期:2006-06-01 00:00:00
abstract::In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6109-6116.1992
更新日期:1992-10-01 00:00:00
abstract::Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.2.750-756.1975
更新日期:1975-11-01 00:00:00
abstract::Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. NE pathogenesis involves the NetB toxin, which is encoded on a large conjugative plasmid within a 42-kb pathogenicity locus. Recent multilocus sequence type (MLST) studies have identified t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01032-12
更新日期:2013-03-01 00:00:00
abstract::Formation of photosynthesis complexes in Rhodobacter sphaeroides is regulated in a redox- and light-dependent manner by the AppA/PpsR and PrrB/PrrA systems. While on the one hand, blue light is sensed by the flavin adenine dinucleotide-binding BLUF domain of AppA, on the other, light is absorbed by bacteriochlorophyll...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00262-09
更新日期:2009-07-01 00:00:00
abstract::The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.338-342.1980
更新日期:1980-07-01 00:00:00