Abstract:
:Deoxycytosine methylase (Dcm) enzyme activity causes mutagenesis in vitro either directly by enzyme-induced deamination of cytosine to uracil in the absence of the methyl donor, S-adenosylmethionine (SAM), or indirectly through spontaneous deamination of [5-methyl]cytosine to thymine. Using a Lac reversion assay, we investigated the contribution of the first mechanism to Dcm mutagenesis in vivo by lowering the levels of SAM. Escherichia coli SAM levels were lowered by reducing SAM synthetase activity via the introduction of a metK84 allele or by hydrolyzing SAM using the bacteriophage T3 SAM hydrolase. The metK84 strains exhibited increased C-to-T mutagenesis. Expression of the T3 SAM hydrolase gene, under the control of the arabinose-inducible P(BAD) promoter, effectively reduced Dcm-mediated genomic DNA methylation. However, increased mutagenesis was not observed until extremely high arabinose concentrations were used, and genome methylation at Dcm sites was negligible.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Macintyre G,Atwood CV,Cupples CGdoi
10.1128/JB.183.3.921-927.2001keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
921-7issue
3eissn
0021-9193issn
1098-5530journal_volume
183pub_type
杂志文章abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.35-41.1985
更新日期:1985-04-01 00:00:00
abstract::We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.626-632.1985
更新日期:1985-05-01 00:00:00
abstract::The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.378-391.1972
更新日期:1972-04-01 00:00:00
abstract::Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.1065-1067.1979
更新日期:1979-09-01 00:00:00
abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7210-7221.1995
更新日期:1995-12-01 00:00:00
abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00426-09
更新日期:2009-08-01 00:00:00
abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00172-15
更新日期:2015-10-01 00:00:00
abstract::Lornitzo, Frank A. (Veterans Administration Hospital, Madison, Wis.), and Dexter S. Goldman. Reversible effect of bicarbonate on the inhibition of mycobacterial and yeast transglucosylases by mycoribnin. J. Bacteriol. 89:1086-1091. 1965.-The transglucosylase which catalyzes the formation of trehalose-6-phosphate from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1086-1091.1965
更新日期:1965-04-01 00:00:00
abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.375-382.1972
更新日期:1972-08-01 00:00:00
abstract::Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1489-1501.1975
更新日期:1975-12-01 00:00:00
abstract::Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.912-921.1990
更新日期:1990-02-01 00:00:00
abstract::We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5462-5469.2000
更新日期:2000-10-01 00:00:00
abstract::Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.3.722-731.1998
更新日期:1998-02-01 00:00:00
abstract::Polysomes were extracted from Bacillus subtilis cells starved for a required amino acid. The monosome peak appeared soon after starvation; no difference in the rate of degradation was detected when the cells were starved for arginine or tryptophan in a double auxotroph. RNA production during starvation was not inhibit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.925-930.1970
更新日期:1970-03-01 00:00:00
abstract::Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06414-11
更新日期:2012-03-01 00:00:00
abstract::The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.534-543.2003
更新日期:2003-01-01 00:00:00
abstract::The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
abstract::Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.1072-1074.1983
更新日期:1983-02-01 00:00:00
abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00799-16
更新日期:2017-05-09 00:00:00
abstract::Lankford, C. E. (The University of Texas, Austin), James R. Walker, James B. Reeves, N. H. Nabbut, B. R. Byers, and R. J. Jones. Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). J. Bacteriol. 91:1070-1079. 1966.-When cells of Bacillus megaterium, grown o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1070-1079.1966
更新日期:1966-03-01 00:00:00
abstract::In cyanobacteria, gene expression is regulated mainly at the level of transcription initiation, which is mediated by the RNA polymerase holoenzyme. The RNA polymerase core is catalytically active, while the sigma factor recognizes promoter sequences. Group 2 sigma factors are similar to the principal sigma factor but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00132-09
更新日期:2009-06-01 00:00:00
abstract::The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01290-07
更新日期:2008-02-01 00:00:00
abstract::The PriA protein, a component of the phiX174-type primosome, was previously shown to be essential for damage-inducible DNA replication in Escherichia coli, termed inducible stable DNA replication. Here, we show that priA::kan null mutants are defective in transductional and conjugational homologous recombination and a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1258-1264.1996
更新日期:1996-03-01 00:00:00
abstract::Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1225-1230.1965
更新日期:1965-05-01 00:00:00
abstract::Six peptidase activities have been distinguished electrophoretically in cell extracts of Salmonella typhimurium with the aid of a histochemical stain. The activities can also be partially separated by chromatography on diethylaminoethyl-cellulose. These peptidases show overlapping substrate specificities. Mutants (pep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.355-363.1974
更新日期:1974-10-01 00:00:00
abstract::Discadenine,3-(3-amino-3-carboxypropyl)-N6-delta 2-isopentenyladenine, which inhibits spore germination, was previously found in Dictyostelium discoideum. Studies on the distribution of discadenine in different species of cellular slime molds by high-pressure liquid chromatography showed that discadenine is present in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.715-719.1980
更新日期:1980-08-01 00:00:00
abstract::Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.18.5573-5584.2003
更新日期:2003-09-01 00:00:00
abstract::Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1503-1513.2002
更新日期:2002-03-01 00:00:00
abstract::For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1165-1167.1984
更新日期:1984-06-01 00:00:00
abstract::A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5138-5143.1996
更新日期:1996-09-01 00:00:00