Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B.

Abstract:

:The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Restriction endonuclease Sau3AI digestions of the cloned hly loci of two independently obtained RV79(Hly+) convertants, when compared with the digests of cloned RV79(Hly-) loci, revealed that an apparent alteration (10 to 15 base pairs) had occurred. In contrast, an apparent 20-base-pair deletion was present in the cloned hly locus of the classical biotype V. cholerae strain 569B. Maxicell analysis and immunoprecipitation of labeled proteins of E. coli which are encoded by the cloned hly loci of RV79(Hly+) and from nuclease BAL 31-deleted plasmids, as well as immunoprecipitation of [35S]methionine-labeled V. cholerae proteins, suggest that the hemolysin is an 84,000-dalton polypeptide.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Goldberg SL,Murphy JR

doi

10.1128/JB.162.1.35-41.1985

subject

Has Abstract

pub_date

1985-04-01 00:00:00

pages

35-41

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

162

pub_type

杂志文章
  • Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction.

    abstract::Whiteley, H. R. (University of Washington, Seattle) and N. G. McCormick. Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction. J. Bacteriol. 85:382-393. 1963.-At an acid pH, extracts of Micrococcus lactilyticus (Veillonella alcalescens) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.382-393.1963

    authors: WHITELEY HR,McCORMICK NG

    更新日期:1963-02-01 00:00:00

  • Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli.

    abstract::The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4631-4640.1990

    authors: Moore RC,Boyle SM

    更新日期:1990-08-01 00:00:00

  • Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene.

    abstract::One of the phosphatidyl glycerophosphate phosphatase genes of Escherichia coli, pgpA, was cloned, and its DNA sequence was determined. Its 507-base-pair open reading frame was consistent with the 18,000-molecular-weight product identified by a maxicell experiment. Between its possible promoter and methionine initiatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5110-5116.1988

    authors: Icho T

    更新日期:1988-11-01 00:00:00

  • Small genes under sporulation control in the Bacillus subtilis genome.

    abstract::Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00534-10

    authors: Schmalisch M,Maiques E,Nikolov L,Camp AH,Chevreux B,Muffler A,Rodriguez S,Perkins J,Losick R

    更新日期:2010-10-01 00:00:00

  • Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus.

    abstract::The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydroly...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.227-233.1970

    authors: Arvidson S,Holme T,Wadström T

    更新日期:1970-10-01 00:00:00

  • Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

    abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1213-1220.1993

    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00

  • Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.

    abstract::The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.2.361-364.1970

    authors: Miao R,Guild WR

    更新日期:1970-02-01 00:00:00

  • Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

    abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01106-08

    authors: Verma A,Cheung AM,Burns DL

    更新日期:2008-11-01 00:00:00

  • Complete genome sequence of the type strain Cupriavidus necator N-1.

    abstract::Here we announce the complete genome sequence of the copper-resistant bacterium Cupriavidus necator N-1, the type strain of the genus Cupriavidus. The genome consists of two chromosomes and two circular plasmids. Based on genome comparison, the chromosomes of C. necator N-1 share a high degree of similarity with the t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05660-11

    authors: Poehlein A,Kusian B,Friedrich B,Daniel R,Bowien B

    更新日期:2011-09-01 00:00:00

  • Quorum-sensing signals and quorum-sensing genes in Burkholderia vietnamiensis.

    abstract::Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.4.1187-1191.2002

    authors: Conway BA,Greenberg EP

    更新日期:2002-02-01 00:00:00

  • Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

    abstract::Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01952-06

    authors: Mishra A,Das A,Cisar JO,Ton-That H

    更新日期:2007-04-01 00:00:00

  • Absence of correlation between rates of cell wall turnover and autolysis shown by Bacillus subtilis mutants.

    abstract::Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.1.318-320.1984

    authors: Vitković L,Cheung HY,Freese E

    更新日期:1984-01-01 00:00:00

  • In vivo definition of an archaeal promoter.

    abstract::We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1844-1849.1995

    authors: Palmer JR,Daniels CJ

    更新日期:1995-04-01 00:00:00

  • Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon.

    abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.245-254.1991

    authors: Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

    更新日期:1991-01-01 00:00:00

  • Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activity.

    abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.303-308.1992

    authors: Vogel AM,Das A

    更新日期:1992-01-01 00:00:00

  • Unusual ribulose 1,5-bisphosphate carboxylase/oxygenase of anoxic Archaea.

    abstract::The predominant pool of organic matter on earth is derived from the biological reduction and assimilation of carbon dioxide gas, catalyzed primarily by the enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). By virtue of its capacity to use molecular oxygen as an alternative and competing gaseous substra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.5.1569-1575.1999

    authors: Watson GM,Yu JP,Tabita FR

    更新日期:1999-03-01 00:00:00

  • Use of an inducible regulatory protein to identify members of a regulon: application to the regulon controlled by the leucine-responsive regulatory protein (Lrp) in Escherichia coli.

    abstract::Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.20.6254-6263.1997

    authors: Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

    更新日期:1997-10-01 00:00:00

  • EXPERIMENTAL VIABLE VACCINE AGAINST PULMONARY COCCIDIOIDOMYCOSIS IN MONKEYS.

    abstract::Converse, John L. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Merida W. Castleberry, and Ernest M. Snyder. Experimental viable vaccine against pulmonary coccidioidomycosis in monkeys. J. Bacteriol. 86:1041-1051. 1963.-Monkeys (Macaca mulatta) vaccinated by subcutaneous injection in the forearm w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.5.1041-1051.1963

    authors: CONVERSE JL,CASTLEBERRY MW,SNYDER EM

    更新日期:1963-11-01 00:00:00

  • Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol.

    abstract::Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.1.22-28.2004

    authors: Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

    更新日期:2004-01-01 00:00:00

  • Involvement of T6 pili in biofilm formation by serotype M6 Streptococcus pyogenes.

    abstract::The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06283-11

    authors: Kimura KR,Nakata M,Sumitomo T,Kreikemeyer B,Podbielski A,Terao Y,Kawabata S

    更新日期:2012-02-01 00:00:00

  • Tra proteins characteristic of F-like type IV secretion systems constitute an interaction group by yeast two-hybrid analysis.

    abstract::Using yeast two-hybrid screens, we have defined an interaction group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili. The six proteins are TraH, TraF, TraW, TraU, TrbI, and TrbB. Except for TrbI, these proteins were all identified as hallmarks of F-like type IV secretion sys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.16.5480-5485.2004

    authors: Harris RL,Silverman PM

    更新日期:2004-08-01 00:00:00

  • LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains.

    abstract::A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.19.5496-5505.2001

    authors: Van Dyk TK,DeRose EJ,Gonye GE

    更新日期:2001-10-01 00:00:00

  • Evidence that specific and "general" control of ornithine carbamoyltransferase production occurs at the level of transcription in Saccharomyces cerevisiae.

    abstract::Ornithine carbamoyltransferase synthesis is subject to two major regulatory systems in Saccharomyces cerevisiae. One system is specific for the arginine biosynthetic enzymes, whereas the other appears to be general, acting on a variety of other amino acid pathways as well. We observed that the synthetic capacity for c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1253-1261.1977

    authors: Messenguy F,Cooper TG

    更新日期:1977-06-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • Characterization of the phd-doc and ccd toxin-antitoxin cassettes from Vibrio superintegrons.

    abstract::Toxin-antitoxin (TA) systems have been reported in the genomes of most bacterial species, and their role when located on the chromosome is still debated. TA systems are particularly abundant in the massive cassette arrays associated with chromosomal superintegrons (SI). Here, we describe the characterization of two su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01389-12

    authors: Guérout AM,Iqbal N,Mine N,Ducos-Galand M,Van Melderen L,Mazel D

    更新日期:2013-05-01 00:00:00

  • Bacillus subtilis pellicle formation proceeds through genetically defined morphological changes.

    abstract::Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00157-07

    authors: Kobayashi K

    更新日期:2007-07-01 00:00:00

  • Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids.

    abstract::Eisenstein, Richard B. (Western Reserve University, Cleveland, Ohio) and Charles Yanofsky. Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids. J. Bacteriol. 83:193-204. 1962-Shigella dysenteriae and Escherichia coli, strains K-12 and B, were found to produce low levels of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.193-204.1962

    authors: EISENSTEIN RB,YANOFSKY C

    更新日期:1962-01-01 00:00:00

  • Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

    abstract::A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.1072-1074.1974

    authors: Lederberg EM,Cohen SN

    更新日期:1974-09-01 00:00:00

  • The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx.

    abstract::The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01289-08

    authors: Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

    更新日期:2009-02-01 00:00:00

  • Purification and characterization of pro-TraTp, the signal sequence-containing precursor of a secreted protein encoded by the F sex factor.

    abstract::The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.464-473.1984

    authors: Minkley EG Jr

    更新日期:1984-05-01 00:00:00