Abstract:
:The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658 amino acids with a molecular size of 73,980 daltons. The molecular weight and predicted ADC amino acid composition are nearly identical to the amino acid analysis of purified ADC performed by Wu and Morris (J. Biol. Chem. 248:1687-1695, 1973). A translational speA-lacZ fusion, pRM65, including 1,389 base pairs (463 amino acids) of the 5' end of speA was constructed. Western blots (immunoblots) with beta-galactosidase antisera revealed two ADC::beta-galactosidase fusion proteins in E. coli bearing pRM65: 160,000 and 156,000 daltons representing precursor and mature hybrid proteins, respectively. The predicted amino acid sequence of ADC contains a region of six amino acid residues found in two bacterial diaminopimelic acid decarboxylases and three eucaryotic ornithine decarboxylases. This conserved sequence is located approximately eight amino acids from the putative pyridoxal phosphate-binding site of ADC and is predicted to be involved in substrate binding.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Moore RC,Boyle SMdoi
10.1128/jb.172.8.4631-4640.1990subject
Has Abstractpub_date
1990-08-01 00:00:00pages
4631-40issue
8eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates b...
journal_title:Journal of bacteriology
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doi:10.1128/JB.146.3.952-964.1981
更新日期:1981-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.170.5.2392-2394.1988
更新日期:1988-05-01 00:00:00
abstract::We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the xylAB regulatory region to the lipase gene from Staphylococcus hyicus were constructed. Repression of lipase activity depend...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.9.3042-3048.1992
更新日期:1992-05-01 00:00:00
abstract::Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-dipho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.185.16.4997-5002.2003
更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.132.1.180-185.1977
更新日期:1977-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.293-296.1980
更新日期:1980-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.170.10.4451-4457.1988
更新日期:1988-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1003-1009.1987
更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.156.1.301-307.1983
更新日期:1983-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.2.711-713.1973
更新日期:1973-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1971-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2742-2748.1988
更新日期:1988-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.177.12.3465-3471.1995
更新日期:1995-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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更新日期:2002-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.941-947.1982
更新日期:1982-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.117.1.29-39.1974
更新日期:1974-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.106-113.1982
更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.177.7.1900-1902.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1570-1577.1994
更新日期:1994-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00445-20
更新日期:2020-09-23 00:00:00
abstract::Dehority, B. A. (Ohio Agricultural Experiment Station, Wooster). Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515-1520. 1965.-Hemicelluloses isolated from flax, corn hulls, alfalfa, oat hulls, and fescue grass were used as sole energy sources ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.6.1515-1520.1965
更新日期:1965-06-01 00:00:00
abstract::The kinetics of peptidoglycan degradation were examined under different conditions of autolysis of Escherichia coli. With cephaloridine- or moenomycin-induced autolysis, degradation did not exceed 25 to 35%, whereas in EDTA-induced autolysis it rapidly reached 65 to 70%. When nonautolyzing cells were fixed overnight w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.627-635.1985
更新日期:1985-02-01 00:00:00