Insertion of transposons through the major cotransduction gap of Escherichia coli K-12.

Abstract:

:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by transduction, is not significantly different from the length obtained by conjugational time of entry. Since this segment of the E. coli chromosome has few known genes, these transposon insertions will be useful for genetic manipulations in the region of the gap. We describe the usefulness of these markers for rapidly mapping mutations which may be isolated in the region from min 27 to 37.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fouts KE,Barbour SD

doi

10.1128/JB.149.1.106-113.1982

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

106-13

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

149

pub_type

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