SAL-TOL in vivo recombinant plasmid pKF439.

Abstract:

:SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL replicon at the position of SmaI-C within XhoI-B of SAL. The molecular size of pKF439 was calculated to be 138 kilobases. pKF439 could be transferred to Pseudomonas putida and Pseudomonas aeruginosa at high frequency, and the transconjugants gained the ability to grow with m-xylene, m-toluate, and salicylate as the sole carbon source.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Furukawa K,Miyazaki T,Tomizuka N

doi

10.1128/JB.162.3.1325-1328.1985

subject

Has Abstract

pub_date

1985-06-01 00:00:00

pages

1325-8

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

162

pub_type

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