Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation.

abstract：:A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...

journal_title：Journal of bacteriology

authors： Novicki TJ,Hecht DW

更新日期：1995-08-01 00:00:00

abstract：:The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

journal_title：Journal of bacteriology

authors： Campbell LA,Yasbin RE

更新日期：1984-10-01 00:00:00

abstract：:The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated tha...

journal_title：Journal of bacteriology

authors： Perry JJ,Evans JB

更新日期：1967-06-01 00:00:00

abstract：:Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...

journal_title：Journal of bacteriology

authors： Hengge R,Larson TJ,Boos W

更新日期：1983-07-01 00:00:00

abstract：:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

journal_title：Journal of bacteriology

authors： Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

更新日期：2005-06-01 00:00:00

abstract：:We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

journal_title：Journal of bacteriology

authors： Flache P,Baumeister R,Hillen W

更新日期：1992-04-01 00:00:00

abstract：:Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiolo...

journal_title：Journal of bacteriology

authors： Slamti L,de Pedro MA,Guichet E,Picardeau M

更新日期：2011-11-01 00:00:00

abstract：:We previously described a cell surface anionic polysaccharide (APS) in Porphyromonas gingivalis that is required for cell integrity and serum resistance. APS is a phosphorylated branched mannan that shares a common epitope with posttranslational additions to some of the Arg-gingipains. This study aimed to determine th...

journal_title：Journal of bacteriology

authors： Rangarajan M,Aduse-Opoku J,Paramonov N,Hashim A,Bostanci N,Fraser OP,Tarelli E,Curtis MA

更新日期：2008-04-01 00:00:00

abstract：:Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...

journal_title：Journal of bacteriology

authors： Zhang C,Zhang M,Ju J,Nietfeldt J,Wise J,Terry PM,Olson M,Kachman SD,Wiedmann M,Samadpour M,Benson AK

更新日期：2003-09-01 00:00:00

abstract：:The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion pla...

journal_title：Journal of bacteriology

authors： Negoro S,Taniguchi T,Kanaoka M,Kimura H,Okada H

更新日期：1983-07-01 00:00:00

abstract：:Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

journal_title：Journal of bacteriology

authors： Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

更新日期：2015-01-01 00:00:00

abstract：:A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...

journal_title：Journal of bacteriology

authors： Wilson RL,Steiert PS,Stauffer GV

更新日期：1993-02-01 00:00:00

abstract：:The membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using Bacillus cereus, B. megaterium, B. subtilis, and Streptococcus faecalis, protoplasts from S. faecalis, and isolated membranes from B. subtilis. Chlorpromazin, nupercain, and tetracain produced characteristic micromorphological...

journal_title：Journal of bacteriology

authors： Silva MT,Sousa JC,Polónia JJ,Macedo PM

更新日期：1979-01-01 00:00:00

abstract：:Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...

journal_title：Journal of bacteriology

authors： Vomvoyanni VE,Argyrakis MP

更新日期：1979-08-01 00:00:00

abstract：:In Escherichia coli and Salmonella, the c-di-GMP effector YcgR inhibits flagellar motility by interacting directly with the motor to alter both its bias and speed. Here, we demonstrate that in both of these bacteria, YcgR acts sequentially, altering motor bias first and then decreasing motor speed. We show that when c...

journal_title：Journal of bacteriology

authors： Nieto V,Partridge JD,Severin GB,Lai RZ,Waters CM,Parkinson JS,Harshey RM

更新日期：2019-12-06 00:00:00

abstract：:A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...

journal_title：Journal of bacteriology

authors： Novick R,Zouzias D,Rush M

更新日期：1975-12-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:Cores are large, rod-shaped structures that have been found almost exclusively in group D streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. This study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal fo...

journal_title：Journal of bacteriology

authors： Coleman SE,Bleiweis AS

更新日期：1977-01-01 00:00:00

abstract：:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

journal_title：Journal of bacteriology

authors： MacPhee KG,Nelson RE,Schuster SM

更新日期：1983-10-01 00:00:00

abstract：:Altering membrane protein and lipid composition is an important strategy for maintaining membrane integrity during environmental stress. Many bacterial small RNAs (sRNAs) control membrane protein production, but sRNA-mediated regulation of membrane fatty acid composition is less well understood. The sRNA RydC was prev...

journal_title：Journal of bacteriology

authors： Bianco CM,Fröhlich KS,Vanderpool CK

更新日期：2019-09-06 00:00:00

abstract：:SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

journal_title：Journal of bacteriology

authors： Cook HA,Kumamoto CA

更新日期：1999-05-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Sulfur-starved cells of Anacystis nidulans have an increased capacity to take up sulfate. The apparent Vmax for sulfate uptake increased at least 10-fold after 24 h of sulfur deprivation, whereas the K1/2 remained unchanged at approximately 1.35 microM. The initial rate of sulfate uptake increased between 2 and 6 h af...

journal_title：Journal of bacteriology

authors： Green LS,Grossman AR

更新日期：1988-02-01 00:00:00

abstract：:We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...

journal_title：Journal of bacteriology

authors： Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MS

更新日期：2004-07-01 00:00:00

abstract：:Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only ...

journal_title：Journal of bacteriology

authors： Englebrecht HL

更新日期：1972-07-01 00:00:00

abstract：:Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

journal_title：Journal of bacteriology

authors： Copeland JC

更新日期：1971-02-01 00:00:00

abstract：:The class 1 outer membrane protein encoded by the porA gene of Neisseria meningitidis is a candidate for a vaccine against meningococcal infection. The expression of class 1 outer membrane protein displays phase variation between three expression levels. Northern (RNA) blot and primer extension analysis revealed that ...

journal_title：Journal of bacteriology

authors： van der Ende A,Hopman CT,Zaat S,Essink BB,Berkhout B,Dankert J

更新日期：1995-05-01 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...

journal_title：Journal of bacteriology

authors： Prüss BM,Campbell JW,Van Dyk TK,Zhu C,Kogan Y,Matsumura P

更新日期：2003-01-01 00:00:00