Abstract:
:A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were present at about 1 copy per chromosome equivalent as compared to 3.6 copies for the same plasmid in its autonomous state. Consistent with this observation was the finding that penicillinase activity was lower for the former strain than for the latter. It was shown further that the plasmid-specific sequences cosedimented on neutral sucrose gradients with fragments of whole-cell DNA many times larger than the plasmid. These two findings were taken as strongly confirmatory of the integrated state. Analysis of whole-cell ribonucleic acid for the presence of plasmid-specific messengers revealed that these were present in approximately the amounts expected on the basis of the DNA study.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Novick R,Zouzias D,Rush Mdoi
10.1128/JB.124.3.1424-1428.1975keywords:
subject
Has Abstractpub_date
1975-12-01 00:00:00pages
1424-8issue
3eissn
0021-9193issn
1098-5530journal_volume
124pub_type
杂志文章abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5818-5830.2005
更新日期:2005-08-01 00:00:00
abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01593-07
更新日期:2008-02-01 00:00:00
abstract::Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00477-09
更新日期:2009-09-01 00:00:00
abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2425-2430.2001
更新日期:2001-04-01 00:00:00
abstract::Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.4.740-748.1963
更新日期:1963-10-01 00:00:00
abstract::The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.202-212.1967
更新日期:1967-07-01 00:00:00
abstract::Chimeric plasmids, which were useful as cloning vehicles in a Streptococcus sanguis (Challis) host vector system, have been constructed. By using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (DNA) fragment bearing an erythromycin resistance determinant was liga...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1425-1435.1980
更新日期:1980-09-01 00:00:00
abstract::Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01120-07
更新日期:2008-04-01 00:00:00
abstract::Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01638-12
更新日期:2012-11-01 00:00:00
abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1144-1155.1973
更新日期:1973-03-01 00:00:00
abstract::The DNA of bacteriophage Mu, extracted from induced lysates, is partially resistant to digestion by the endonuclease BalI. This modification of DNA is controlled by the Mu modification function (mom), which acts in conjunction with the dam (DNA-adenine methylation) function of Escherichia coli. Since the BalI recognit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.423-428.1978
更新日期:1978-10-01 00:00:00
abstract::Dowell, C. E. (The University of Texas, Dallas) and E. D. Rosenblum. Staphylococcal transducing particle. J. Bacteriol. 84:1076-1079. 1962.-When novobiocin-resistant transductants were isolated under conditions that permitted superinfection, almost all the clones were lysogenic for the transducing phage. If superinfec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.1076-1079.1962
更新日期:1962-11-01 00:00:00
abstract::A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.549-556.1986
更新日期:1986-02-01 00:00:00
abstract::The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.534-543.2003
更新日期:2003-01-01 00:00:00
abstract::Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00665-18
更新日期:2019-03-13 00:00:00
abstract::Brubaker, Robert R. (Fort Detrick, Frederick, Md.) and Michael J. Surgalla. Pesticins. II. Production of pesticin I and II. J. Bacteriol. 84:539-545. 1962.-Pesticin I was separated from pesticin I inhibitor by ion-exchange chromatography of cell-free culture supernatant fluids and by acid precipitation of soluble prep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.3.539-545.1962
更新日期:1962-09-01 00:00:00
abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1121-1128.1977
更新日期:1977-02-01 00:00:00
abstract::Mycoplasmas that are known to exhibit gliding motility possess a differentiated tip structure. This polar organelle mediates cytadherence and gliding motor activity and contains a cytoskeleton-like component that provides structural support. Here, we describe gliding motility and a unique cytoskeleton in Mycoplasma in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00474-09
更新日期:2009-08-01 00:00:00
abstract::Three gene libraries of Bordetella avium 197 DNA were prepared in Escherichia coli LE392 by using the cosmid vectors pCP13 and pYA2329, a derivative of pCP13 specifying spectinomycin resistance. The cosmid libraries were screened with convalescent-phase anti-B. avium turkey sera and polyclonal rabbit antisera against ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.23.7729-7742.1992
更新日期:1992-12-01 00:00:00
abstract::Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2773-2782.1989
更新日期:1989-05-01 00:00:00
abstract::The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.11.3328-3335.2001
更新日期:2001-06-01 00:00:00
abstract::Vi-agglutinin, active cutaneous anaphylaxis and protective responses (ed(50)) of three mouse strains (CFW, NIH, and Balb/cAnN) to acetone-inactivated typhoid vaccine and soluble Vi antigen were compared. Seven days after immunization with either typhoid vaccine or Vi antigen the three strains of mice differed with res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.8-12.1969
更新日期:1969-07-01 00:00:00
abstract::Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two diff...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01949-06
更新日期:2007-05-01 00:00:00
abstract::A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2793-2803.1987
更新日期:1987-06-01 00:00:00
abstract::The PRO1 gene of Saccharomyces cerevisiae encodes the 428-amino-acid protein gamma-glutamyl kinase (ATP:L-glutamate 5-phosphotransferase, EC 2.7.2.11), which catalyzes the first step in proline biosynthesis. Amino acid sequence comparison revealed significant homology between the yeast and Escherichia coli gamma-gluta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.4148-4156.1992
更新日期:1992-06-01 00:00:00
abstract::Using transposon Tn5-mediated mutagenesis, an essential Rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifA. Maxicell and DNA sequence analysis demonstrated that the new gene is transcribed in the same direction as nifA and codes for a 54-kilodalto...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1120-1126.1987
更新日期:1987-03-01 00:00:00
abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2835-2841.1989
更新日期:1989-05-01 00:00:00
abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6970-6982.2004
更新日期:2004-10-01 00:00:00
abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5508-5512.2002
更新日期:2002-10-01 00:00:00