Separation of chromosome termini during sporulation of Bacillus subtilis depends on SpoIIIE.

Abstract:

:Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two different probes were used to study the behavior of the terminus (ter) region of the chromosome during spore formation. Only one ter region was observed at the time of sporulation division. A second ter region, indicative of chromosome separation, was not distinguishable until engulfment was nearing completion, when one was in the mother cell and the other in the prespore. Separation of the two ter regions depended on the DNA translocase SpoIIIE. It is concluded that SpoIIIE is required during spore formation for chromosome separation as well as for translocation; SpoIIIE is not required for separation during vegetative growth.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bogush M,Xenopoulos P,Piggot PJ

doi

10.1128/JB.01949-06

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

3564-72

issue

9

eissn

0021-9193

issn

1098-5530

pii

JB.01949-06

journal_volume

189

pub_type

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