Abstract:
:A mutation in Escherichia coli leads to the loss of ribosomal protein L24, severely impaired growth, and a temperature-sensitive phenotype. The mutation was shown to be in rplX, the gene for protein L24, and was due to the alteration of an AAA codon to a TAA stop codon at position 61 in rplX that resulted in a 20-amino acid peptide instead of the 104 amino acids of wild-type L24 protein. rplX genes from three temperature-resistant and fast growing pseudorevertants of the mutant were cloned and sequenced. They were found to have different base substitutions in the TAA codon, resulting in the reappearance of a full-sized protein L24 moiety. Complementation of the slow growth in trans could be achieved with several plasmids containing at least the spc promoter and intact L14 and L24 genes. Plasmids containing genes distal to rplX could further stimulate growth, and the wild type arose when the entire spc operon and the alpha operon were present. In all cases, protein L24 was expressed by the plasmids. Therefore, slow growth could be explained by polarity extending to the alpha operon. However, temperature sensitivity could not be complemented by any of the plasmids in trans, although we found that this phenotype was caused by the mutation in the rplX gene.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Nishi K,Dabbs ER,Schnier Jdoi
10.1128/JB.163.3.890-894.1985subject
Has Abstractpub_date
1985-09-01 00:00:00pages
890-4issue
3eissn
0021-9193issn
1098-5530journal_volume
163pub_type
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更新日期:1987-11-01 00:00:00
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更新日期:1963-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.178.7.1793-1799.1996
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更新日期:1989-04-01 00:00:00
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更新日期:1972-11-01 00:00:00
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更新日期:1969-01-01 00:00:00
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更新日期:2003-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1977-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1996-09-01 00:00:00
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更新日期:2003-01-01 00:00:00
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更新日期:2001-05-01 00:00:00