Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii.


:Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the enzyme GDP-mannose dehydrogenase (algD), which is the key enzyme for alginate synthesis in Pseudomonas aeruginosa. This gene has a high degree of similarity with the algD gene from P. aeruginosa, and similar proteins seem to be involved in algD regulation in both bacteria. We show the existence of two mRNA start sites; one of these sites corresponds to a promoter transcribed by RNA polymerase containing a sigma E subunit. An A. vinelandii algD mutant which is completely impaired in alginate production and which is unable to form desiccation-resistant cells was constructed. The effects of NH4, NO3, and NaCl concentrations on algD transcription for three A. vinelandii strains producing different alginate levels were evaluated. We found a strict correlation between alginate production and algD transcription for the three strains studied; however, the effects on algD transcription under the conditions studied were different for each strain. The nitrogen source regulates algD expression in the wild-type strain.


J Bacteriol


Journal of bacteriology


Campos M,Martínez-Salazar JM,Lloret L,Moreno S,Núñez C,Espín G,Soberón-Chávez G




Has Abstract


1996-04-01 00:00:00












  • Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.

    abstract::The pathway of anaerobic toluene oxidation to benzoyl coenzyme A (benzoyl-CoA) consists of an initial reaction catalyzed by benzylsuccinate synthase, a glycyl radical enzyme adding the methyl group of toluene to the double bond of a fumarate cosubstrate, and a subsequent beta-oxidation pathway of benzylsuccinate. Benz...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leuthner B,Heider J

    更新日期:2000-01-01 00:00:00

  • The Bacillus subtilis GntR family repressor YtrA responds to cell wall antibiotics.

    abstract::The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

    更新日期:2011-10-01 00:00:00

  • Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the beta subclass.

    abstract::Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hurek T,Egener T,Reinhold-Hurek B

    更新日期:1997-07-01 00:00:00

  • Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast.

    abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lorenz RT,Parks LW

    更新日期:1987-08-01 00:00:00

  • Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state.

    abstract::Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-04-01 00:00:00

  • Structures containing polyphosphate in Micrococcus lysodeikticus.

    abstract::Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Friedberg I,Avigad G

    更新日期:1968-08-01 00:00:00

  • Characterization of EvgAS-YdeO-GadE branched regulatory circuit governing glutamate-dependent acid resistance in Escherichia coli.

    abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ma Z,Masuda N,Foster JW

    更新日期:2004-11-01 00:00:00

  • Molecular mapping of glyW, a duplicate gene for tRNA3Gly of Escherichia coli.

    abstract::By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

    更新日期:1982-11-01 00:00:00

  • L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.

    abstract::Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mansilla MC,de Mendoza D

    更新日期:1997-02-01 00:00:00

  • Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.

    abstract::DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chen YM,Backman K,Magasanik B

    更新日期:1982-04-01 00:00:00

  • Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates.

    abstract::In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

    更新日期:2011-10-01 00:00:00

  • Mutants of yeast sensitive to ultraviolet light.

    abstract::Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Snow R

    更新日期:1967-09-01 00:00:00

  • Formation of protoplasts from resting spores.

    abstract::Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fitz-James PC

    更新日期:1971-03-01 00:00:00

  • Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

    abstract::Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Southworth MW,Perler FB

    更新日期:2002-11-01 00:00:00

  • Nature of the complementation products formed by a complementing mutant of neurospora crassa.

    abstract::The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sundaram TK,Fincham JR

    更新日期:1968-03-01 00:00:00

  • Cell-free amino acid-incorporating system from Pseudomonas indigofera.

    abstract::Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shiio T,McFadden BA

    更新日期:1965-10-01 00:00:00

  • Abortive infection mechanisms and prophage sequences significantly influence the genetic makeup of emerging lytic lactococcal phages.

    abstract::In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Labrie SJ,Moineau S

    更新日期:2007-02-01 00:00:00

  • Crl binds to domain 2 of σ(S) and confers a competitive advantage on a natural rpoS mutant of Salmonella enterica serovar Typhi.

    abstract::The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Monteil V,Kolb A,Mayer C,Hoos S,England P,Norel F

    更新日期:2010-12-01 00:00:00

  • Mutation of the murC and murB Genes Impairs Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

    abstract:UNLABELLED:To stabilize cellular integrity in the face of environmental perturbations, most bacteria, including cyanobacteria, synthesize and maintain a strong, flexible, three-dimensional peptidoglycan lattice. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium capable of differentiating morphologically dist...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Videau P,Rivers OS,Ushijima B,Oshiro RT,Kim MJ,Philmus B,Cozy LM

    更新日期:2016-03-31 00:00:00

  • Loss of 4.5S RNA induces the heat shock response and lambda prophage in Escherichia coli.

    abstract::During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

    更新日期:1990-02-01 00:00:00

  • Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen.

    abstract::Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

    更新日期:2011-10-01 00:00:00

  • A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength.

    abstract::Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Csonka LN

    更新日期:1982-09-01 00:00:00

  • Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber.

    abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

    更新日期:1994-07-01 00:00:00

  • Purification and characterization of specific 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Escherichia coli B.

    abstract::A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ray PH,Benedict CD

    更新日期:1980-04-01 00:00:00

  • Clonal diversity and host distribution in Bordetella bronchiseptica.

    abstract::A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Musser JM,Bemis DA,Ishikawa H,Selander RK

    更新日期:1987-06-01 00:00:00


    abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1963-08-01 00:00:00

  • Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

    abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

    更新日期:1990-01-01 00:00:00

  • Transcriptional control of the sulfur-regulated cysH operon, containing genes involved in L-cysteine biosynthesis in Bacillus subtilis.

    abstract::The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mansilla MC,Albanesi D,de Mendoza D

    更新日期:2000-10-01 00:00:00

  • Thermal death of a hydrocarbon bacterium in a nonaqueous fluid.

    abstract::A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Severance MM,LaRock PA

    更新日期:1973-12-01 00:00:00

  • Properties of D-arabinose isomerase purified from two strains of Escherichia coli.

    abstract::d-Arabinose isomerase (EC has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Boulter JR,Gielow WO

    更新日期:1973-02-01 00:00:00