Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii.

abstract：:The pathway of anaerobic toluene oxidation to benzoyl coenzyme A (benzoyl-CoA) consists of an initial reaction catalyzed by benzylsuccinate synthase, a glycyl radical enzyme adding the methyl group of toluene to the double bond of a fumarate cosubstrate, and a subsequent beta-oxidation pathway of benzylsuccinate. Benz...

journal_title：Journal of bacteriology

authors： Leuthner B,Heider J

更新日期：2000-01-01 00:00:00

abstract：:The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

journal_title：Journal of bacteriology

authors： Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

更新日期：2011-10-01 00:00:00

abstract：:Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

journal_title：Journal of bacteriology

authors： Hurek T,Egener T,Reinhold-Hurek B

更新日期：1997-07-01 00:00:00

abstract：:Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...

journal_title：Journal of bacteriology

authors： Lorenz RT,Parks LW

更新日期：1987-08-01 00:00:00

abstract：:Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

journal_title：Journal of bacteriology

authors： JONES LM,McDUFF CR,WILSON JB

更新日期：1962-04-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

journal_title：Journal of bacteriology

authors： Ma Z,Masuda N,Foster JW

更新日期：2004-11-01 00:00:00

abstract：:By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

journal_title：Journal of bacteriology

authors： Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

更新日期：1982-11-01 00:00:00

abstract：:Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

journal_title：Journal of bacteriology

authors： Mansilla MC,de Mendoza D

更新日期：1997-02-01 00:00:00

abstract：:DNA was prepared from a strain of Escherichia coli bearing a mutation which confers the GlnC phenotype (inability to reduce the expression of glnA and other nitrogen-regulated operons in response to ammonia in the growth medium). A fragment of this DNA carrying glnA, the structural gene for glutamine synthetase, was c...

journal_title：Journal of bacteriology

authors： Chen YM,Backman K,Magasanik B

更新日期：1982-04-01 00:00:00

abstract：:In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

journal_title：Journal of bacteriology

authors： Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

更新日期：2011-10-01 00:00:00

abstract：:Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

journal_title：Journal of bacteriology

authors： Snow R

更新日期：1967-09-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...

journal_title：Journal of bacteriology

authors： Southworth MW,Perler FB

更新日期：2002-11-01 00:00:00

abstract：:The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

journal_title：Journal of bacteriology

authors： Sundaram TK,Fincham JR

更新日期：1968-03-01 00:00:00

abstract：:Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in...

journal_title：Journal of bacteriology

authors： Shiio T,McFadden BA

更新日期：1965-10-01 00:00:00

abstract：:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

journal_title：Journal of bacteriology

authors： Labrie SJ,Moineau S

更新日期：2007-02-01 00:00:00

abstract：:The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...

journal_title：Journal of bacteriology

authors： Monteil V,Kolb A,Mayer C,Hoos S,England P,Norel F

更新日期：2010-12-01 00:00:00

abstract：UNLABELLED:To stabilize cellular integrity in the face of environmental perturbations, most bacteria, including cyanobacteria, synthesize and maintain a strong, flexible, three-dimensional peptidoglycan lattice. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium capable of differentiating morphologically dist...

journal_title：Journal of bacteriology

authors： Videau P,Rivers OS,Ushijima B,Oshiro RT,Kim MJ,Philmus B,Cozy LM

更新日期：2016-03-31 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

journal_title：Journal of bacteriology

authors： Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

更新日期：2011-10-01 00:00:00

abstract：:Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...

journal_title：Journal of bacteriology

authors： Csonka LN

更新日期：1982-09-01 00:00:00

abstract：:The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

journal_title：Journal of bacteriology

authors： Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

更新日期：1994-07-01 00:00:00

abstract：:A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

journal_title：Journal of bacteriology

authors： Ray PH,Benedict CD

更新日期：1980-04-01 00:00:00

abstract：:A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

journal_title：Journal of bacteriology

authors： Musser JM,Bemis DA,Ishikawa H,Selander RK

更新日期：1987-06-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

journal_title：Journal of bacteriology

authors： DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

更新日期：1963-08-01 00:00:00

abstract：:The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

journal_title：Journal of bacteriology

authors： Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

更新日期：1990-01-01 00:00:00

abstract：:The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...

journal_title：Journal of bacteriology

authors： Mansilla MC,Albanesi D,de Mendoza D

更新日期：2000-10-01 00:00:00

abstract：:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

journal_title：Journal of bacteriology

authors： Severance MM,LaRock PA

更新日期：1973-12-01 00:00:00

abstract：:d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

journal_title：Journal of bacteriology

authors： Boulter JR,Gielow WO

更新日期：1973-02-01 00:00:00