Lysine decarboxylase mutants of Escherichia coli: evidence for two enzyme forms.

abstract：:Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schnaitman CA

更新日期：1978-03-01 00:00:00

abstract：:Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of...

journal_title：Journal of bacteriology

authors： Hilger F,Mortimer RK

更新日期：1980-01-01 00:00:00

abstract：:The ornithine lipid of Thiobacillus thiooxidans was found to be 1.9% of the total polar lipids. Approximately 80% of this lipid was found to be localized in the outer membrane. ...

journal_title：Journal of bacteriology

authors： Dees C,Shively JM

更新日期：1982-02-01 00:00:00

abstract：:The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low ...

journal_title：Journal of bacteriology

authors： Armstrong SK,Parr TR Jr,Parker CD,Hancock RE

更新日期：1986-04-01 00:00:00

abstract：:The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress. In response to reduced water activity, P. putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatible solute identified as mannitol (using 13C...

journal_title：Journal of bacteriology

authors： Kets EP,Galinski EA,de Wit M,de Bont JA,Heipieper HJ

更新日期：1996-12-01 00:00:00

abstract：:The rcs phosphorelay pathway components were originally identified as regulators of capsule synthesis. In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed r...

journal_title：Journal of bacteriology

authors： Majdalani N,Heck M,Stout V,Gottesman S

更新日期：2005-10-01 00:00:00

abstract：:Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...

journal_title：Journal of bacteriology

authors： Kurushima J,Nakane D,Nishizaka T,Tomita H

更新日期：2015-01-01 00:00:00

abstract：:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

journal_title：Journal of bacteriology

authors： Sánchez L,Pan W,Viñas M,Nikaido H

更新日期：1997-11-01 00:00:00

abstract：:We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...

journal_title：Journal of bacteriology

authors： Tsui HC,Arps PJ,Connolly DM,Winkler ME

更新日期：1991-11-01 00:00:00

abstract：:The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

journal_title：Journal of bacteriology

authors： Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

更新日期：1982-12-01 00:00:00

abstract：:Exponentially growing cells of the gram-negative bacterium Chromobacterium violaceum demonstrate invaginations of the cytoplasmic membrane with a high frequency. These invaginations conform to the ultrastructural appearance of mesosomes of gram-positive bacteria. As many as four mesosomes are observed per cell, each o...

journal_title：Journal of bacteriology

authors： Rucinsky TE,Cota-Robles EH

更新日期：1974-05-01 00:00:00

abstract：:With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

journal_title：Journal of bacteriology

authors： Potter SS,Bott KF,Newbold JE

更新日期：1977-01-01 00:00:00

abstract：:A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...

journal_title：Journal of bacteriology

authors： Wood DO,Brownell GH

更新日期：1975-02-01 00:00:00

abstract：:Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...

journal_title：Journal of bacteriology

authors： Martin K,Morlin G,Smith A,Nordyke A,Eisenstark A,Golomb M

更新日期：1998-01-01 00:00:00

abstract：:Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...

journal_title：Journal of bacteriology

authors： Kusuya Y,Kurokawa K,Ishikawa S,Ogasawara N,Oshima T

更新日期：2011-06-01 00:00:00

abstract：:Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

journal_title：Journal of bacteriology

authors： Popham DL,Meador-Parton J,Costello CE,Setlow P

更新日期：1999-10-01 00:00:00

abstract：:During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...

journal_title：Journal of bacteriology

authors： Guedon E,Desvaux M,Petitdemange H

更新日期：2000-04-01 00:00:00

abstract：:Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

journal_title：Journal of bacteriology

authors： Mallette MF

更新日期：1967-08-01 00:00:00

abstract：:Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....

journal_title：Journal of bacteriology

authors： NAKATA HM

更新日期：1963-09-01 00:00:00

abstract：:Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...

journal_title：Journal of bacteriology

authors： Gaffar A,Terry DR,Sagers RD

更新日期：1966-04-01 00:00:00

abstract：:Haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane proteins P2 and P5, are kn...

journal_title：Journal of bacteriology

authors： Mullins MA,Register KB,Bayles DO,Loving CL,Nicholson TL,Brockmeier SL,Dyer DW,Phillips GJ

更新日期：2009-10-01 00:00:00

abstract：:The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a pote...

journal_title：Journal of bacteriology

authors： Peciña A,Pascual A,Paneque A

更新日期：1999-03-01 00:00:00

abstract：:Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-ace...

journal_title：Journal of bacteriology

authors： Tisa LS,Cotter DA

更新日期：1980-02-01 00:00:00

abstract：:A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

journal_title：Journal of bacteriology

authors： Von Tersch MA,Carlton BC

更新日期：1984-12-01 00:00:00

abstract：:Analysis of strains with deletions of all or part of nif have ordered the Klebsiella pneumoniae genetic loci as thi rbt dal udk gnd rfb has nif shiA. The his-nif plasmids pRD1 and pTM4010 contain the genes gnd rfb his nif shiA. ...

journal_title：Journal of bacteriology

authors： MacNeil D,Supiano MA,Brill WJ

更新日期：1979-06-01 00:00:00

abstract：:Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

journal_title：Journal of bacteriology

authors： Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

更新日期：2000-07-01 00:00:00

abstract：:There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

journal_title：Journal of bacteriology

authors： Nagarajan S,Sherman DM,Shaw I,Sherman LA

更新日期：2012-01-01 00:00:00

abstract：:During growth of streptomycin-dependent strains of Escherichia coli in the absence of streptomycin (deprived growth), both constitutive and inducible synthesis of beta-galactosidase were preferentially inhibited. A similar preferential inhibition of constitutive and derepressed synthesis of alkaline phosphatase was ob...

journal_title：Journal of bacteriology

authors： Goodman RE,Spotts CR

更新日期：1967-10-01 00:00:00

abstract：:A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...

journal_title：Journal of bacteriology

authors： Yoshikawa H,Jeong SM,Hirata A,Kawamura F,Doi RH,Takahashi H

更新日期：1993-06-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00