The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump.

Abstract:

:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these genes are expressed and contribute to the baseline level resistance of this organism through active drug efflux. The gene disruption did not produce detectable changes in susceptibility to several other antibiotics, possibly because rapid influx of small antibiotic molecules through the large H. influenzae porin channels counterbalances their efflux.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sánchez L,Pan W,Viñas M,Nikaido H

doi

10.1128/jb.179.21.6855-6857.1997

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

6855-7

issue

21

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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