Abstract:
:Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily of eubacterial RNA polymerase sigma and anti-sigma factors, respectively. This group of sigma factors has been shown to regulate gene expression in response to stress conditions. sigV and rsiV were shown to be under the control of the same promoter. The transcriptional start site was determined, and the 1.5-kb mRNA transcript was shown to be overexpressed under glucose and complete starvation, as well as under physicochemical treatments. Three mutants, affected in sigV, rsiV, and both genes, were constructed by double-crossover recombination within the genome of E. faecalis strain JH2-2. Compared with the wild type and the rsiV mutant, the sigV mutants were more susceptible to heat shock, acid, and ethanol treatments and displayed decreased survival during long-term starvation. A nisin-inducible sigV gene construction used in complementation assays restored the wild phenotype of the sigV mutants, confirming the involvement of SigV in the heat shock, ethanol, and acid stress responses. Northern blot analysis carried out with the three mutant strains revealed the inhibition of sigV expression by the related anti-sigma factor gene rsiV. In addition, putative candidates of the sigV regulon determined by computer search for the sigV promoter sequence were analyzed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke Adoi
10.1128/JB.187.3.1022-1035.2005keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
1022-35issue
3eissn
0021-9193issn
1098-5530pii
187/3/1022journal_volume
187pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.127.1.268-280.1976
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pub_type: 杂志文章
doi:10.1128/JB.164.1.237-244.1985
更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3468-3478.1988
更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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更新日期:1980-08-01 00:00:00
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更新日期:1975-12-01 00:00:00
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更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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更新日期:2002-11-01 00:00:00
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更新日期:1997-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.116.2.663-672.1973
更新日期:1973-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.139-146.1974
更新日期:1974-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.182.16.4466-4477.2000
更新日期:2000-08-01 00:00:00