Abstract:
:The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 and its mutant DL-54, which is deficient in Ca(2+)- and Mg(2+)-stimulated adenosine 5'-triphosphatase (EC 3.6.1.3) activity. It is concluded that active transport of glycylglycine, like other shock-sensitive transport systems, has an obligatory requirement for phosphate bond energy, but not for respiration or the energized state of the membrane. The major evidence for this conclusion is as follows. (i) Uptake of glycylglycine is severely inhibited by arsenate. (ii) Oxidizable energy sources such as d-lactate, succinate, and ascorbate, which is mediated by N-methylphenazinium methylsulfate, cannot serve as energy sources for the transport of glycylglycine in DL-54, which lacks oxidative phosphorylation. (iii) When energy is supplied only from adenosine-5'-triphosphate produced by glycolysis (anaerobic transport assays with glucose as the energy source in DL-54), substantial uptake of glycylglycine is observed. (iv) When the Ca(2+)-Mg(2+)-adenosine triphosphatase activity is absent but substrate-level phosphorylations and electron transport are operating (glucose as the energy source in DL-54), transport of glycylglycine shows significant resistance to the uncouplers, dinitrophenol and carbonyl cyanide-p-trifluoromethoxyphenylhydrazone.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cowell JLdoi
10.1128/JB.120.1.139-146.1974subject
Has Abstractpub_date
1974-10-01 00:00:00pages
139-46issue
1eissn
0021-9193issn
1098-5530journal_volume
120pub_type
杂志文章abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...
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doi:10.1128/JB.120.1.565-567.1974
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1223-1233.1980
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pub_type: 杂志文章
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doi:10.1128/jb.171.6.3440-3448.1989
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journal_title:Journal of bacteriology
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doi:10.1128/JB.186.16.5292-5302.2004
更新日期:2004-08-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.175.23.7697-7701.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1329-1338.1983
更新日期:1983-06-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.108.3.973-979.1971
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journal_title:Journal of bacteriology
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更新日期:1990-02-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.175.10.3228-3231.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1986-03-01 00:00:00
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更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.126.2.661-667.1976
更新日期:1976-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-09-01 00:00:00
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更新日期:1987-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.174.19.6109-6116.1992
更新日期:1992-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1994-04-01 00:00:00
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更新日期:2005-05-01 00:00:00