Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.

abstract：:The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a pote...

journal_title：Journal of bacteriology

authors： Peciña A,Pascual A,Paneque A

更新日期：1999-03-01 00:00:00

abstract：:Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...

journal_title：Journal of bacteriology

authors： Finkenwirth F,Kirsch F,Eitinger T

更新日期：2013-09-01 00:00:00

abstract：:Hyaluronate stimulated the formation of exolipase by Serratia marcescens. This ability was abolished when all carboxyl groups of hyaluronate were methyl esterified. Additional studies suggested that the biological inactivity of esterified hyaluronate should be ascribed to the reduced conformational order of the molecu...

journal_title：Journal of bacteriology

authors： Jäger KE,Winkler UK

更新日期：1979-09-01 00:00:00

abstract：:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

journal_title：Journal of bacteriology

authors： Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

更新日期：2005-06-01 00:00:00

abstract：:Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...

journal_title：Journal of bacteriology

authors： Sheehan SM,Switzer RL

更新日期：1990-01-01 00:00:00

abstract：:The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...

journal_title：Journal of bacteriology

authors： Seeboth PG,Bohnsack K,Hollenberg CP

更新日期：1990-02-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：:In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

journal_title：Journal of bacteriology

authors： Ray PH,Lillich TT,White DC

更新日期：1972-10-01 00:00:00

abstract：:Mitospores or cysts of Allomyces macrogynus do not take up the glucose analogue 2-deoxyglucose. Uptake of 2-deoxyglucose by germlings begins at 25 min into germination, the start of the rhizoid stage, and increases in rate by approximately 50-fold until 100 min into germination. The rate remains constant from 100 to 2...

journal_title：Journal of bacteriology

authors： Burke DD

更新日期：1975-11-01 00:00:00

abstract：:Acetate kinase catalyzes the reversible phosphorylation of acetate (CH3COO- + ATP<-->CH3CO2PO3(2-) + ADP). A mechanism which involves a covalent phosphoryl-enzyme intermediate has been proposed, and chemical modification studies of the enzyme from Escherichia coli indicate an unspecified glutamate residue is phosphory...

journal_title：Journal of bacteriology

authors： Singh-Wissmann K,Ingram-Smith C,Miles RD,Ferry JG

更新日期：1998-03-01 00:00:00

abstract：:Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

journal_title：Journal of bacteriology

authors： LYONS AJ Jr,PRIDHAM TG

更新日期：1962-02-01 00:00:00

abstract：:The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

journal_title：Journal of bacteriology

authors： Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

更新日期：2008-09-01 00:00:00

abstract：:Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...

journal_title：Journal of bacteriology

authors： Prakash-Cheng A,Ryu J

更新日期：1993-08-01 00:00:00

abstract：:Peptidoglycan synthesis rate in nonconstricting filaments of Escherichia coli dnaX(Ts) has been studied by autoradiography of incorporated [3H]diaminopimelic acid. Analysis of autoradiograms of whole cells and sacculi showed that peptidoglycan is synthesized at a reduced rate in the nucleoid-containing parts of these ...

journal_title：Journal of bacteriology

authors： Mulder E,Woldringh CL

更新日期：1991-08-01 00:00:00

abstract：:Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

journal_title：Journal of bacteriology

authors： Cerda-Maira FA,Ringelberg CS,Taylor RK

更新日期：2008-11-01 00:00:00

abstract：:Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

journal_title：Journal of bacteriology

authors： McCarthy D,Kupfer DM

更新日期：1987-02-01 00:00:00

abstract：:In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1974-01-01 00:00:00

abstract：:At some time during sporulation development, cells of Bacillus subtilis develop a commitment to continue sporulation even after addition of or dilution into a fresh nutrient. The extent of commitment was measured by the titer of spores produced at the time at which the original culture sporulated maximally. Since newl...

journal_title：Journal of bacteriology

authors： Cooney PH,Whiteman PF,Freese E

更新日期：1977-02-01 00:00:00

abstract：:The transcriptional control of the kdpFABC (K+ transport) operon of Salmonella typhimurium was characterized with a lacZ fusion. The kdpFABC operon of this organism was induced by K+ limitation and high osmolality, and osmotic induction was antagonized by a high concentration of K+. In the trkA (sapG) kdp+ mutant back...

journal_title：Journal of bacteriology

authors： Frymier JS,Reed TD,Fletcher SA,Csonka LN

更新日期：1997-05-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00

abstract：:The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...

journal_title：Journal of bacteriology

authors： Calvio C,Celandroni F,Ghelardi E,Amati G,Salvetti S,Ceciliani F,Galizzi A,Senesi S

更新日期：2005-08-01 00:00:00

abstract：:It was shown in an accompanying paper (Buck and Groman, J. Bacteriol. 148: 131-142, 1981) that gamma-tsr-1 phage stocks produced by heat induction of lysogens are a mixture of two phages which differ in the content of their deoxyribonucleic acid (DNA). This difference is evidenced by the appearance of "heterogeneous" ...

journal_title：Journal of bacteriology

authors： Buck GA,Groman NB

更新日期：1981-10-01 00:00:00

abstract：:Using a sensitive primer extension technique, we have carried out studies to localize the start site of replication of the replicon RepFIC. In the course of these studies, we have found evidence that supports the hypothesis that transcription is an integral component of the initiation of replication. On the basis of o...

journal_title：Journal of bacteriology

authors： Maas R,Wang C

更新日期：1997-04-01 00:00:00

abstract：:Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

journal_title：Journal of bacteriology

authors： Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

更新日期：1998-08-01 00:00:00

abstract：:In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

journal_title：Journal of bacteriology

authors： Frías JE,Flores E

更新日期：2010-06-01 00:00:00

abstract：:Some lines of the widely used strain CR34 of Escherichia coli K-12 carry a plasmid. The plasmid has a mass of 12 +/- 2 X 10(6) megadaltons and is maintained at a low copy number per cell (1 to 2), and this number is not amplified by growth of cells in the presence of chloramphenicol. The plasmid is designated as pCU3....

journal_title：Journal of bacteriology

authors： Wang PY,Relf J,Palchaudhuri S,Iyer VN

更新日期：1978-02-01 00:00:00

abstract：:The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...

journal_title：Journal of bacteriology

authors： Osborne C,Chen LM,Matthews RG

更新日期：1991-03-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3...

journal_title：Journal of bacteriology

authors： Wisniewski JA,Teng WL,Bannam TL,Rood JI

更新日期：2015-02-15 00:00:00

abstract：:We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

journal_title：Journal of bacteriology

authors： Wang YL,Beach MJ,Rodwell VW

更新日期：1989-10-01 00:00:00