Abstract:
:The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form had a molecular weight (66,400) and a composition in close agreement with that determined for the purified receptor. The distribution of amino acids in the receptor protein was similar to that of other outer membrane proteins, showing a fairly even distribution of charged residues and the absence of extensive hydrophobic stretches. The btuB451 mutation appears to alter the receptor to eliminate its ability to function in vitamin B12 uptake without affecting its ligand binding properties. The sequence of the DNA from this mutant was determined and revealed a leucine-to-proline (C-to-T transition) change in the eighth amino acid of the mature form.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Heller K,Kadner RJdoi
10.1128/JB.161.3.904-908.1985subject
Has Abstractpub_date
1985-03-01 00:00:00pages
904-8issue
3eissn
0021-9193issn
1098-5530journal_volume
161pub_type
杂志文章abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01579-14
更新日期:2014-08-01 00:00:00
abstract::Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4700-4706.1991
更新日期:1991-08-01 00:00:00
abstract::The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.3885-3894.1994
更新日期:1994-07-01 00:00:00
abstract::Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.840-843.1974
更新日期:1974-09-01 00:00:00
abstract::This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.635-641.1977
更新日期:1977-05-01 00:00:00
abstract::The mannans of Saccharomyces chevalieri, S. italicus, S. diastaticus, and S. carlsbergensis, were acetolyzed, and the fragments were separated by gel filtration. All gave similar acetolysis fingerprints, which were distinguished from S. cerevisiae by the presence of a pentasaccharide component in addition to the mono-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.461-467.1974
更新日期:1974-02-01 00:00:00
abstract::A circular genetic map of Bacillus stearothermophilus NUB36 was constructed by transduction with bacteriophage TP-42C and protoplast fusion. Sixty-four genes were tentatively assigned a cognate Bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.793-801.1990
更新日期:1990-02-01 00:00:00
abstract::Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4011-4017.1987
更新日期:1987-09-01 00:00:00
abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02051-12
更新日期:2013-02-01 00:00:00
abstract::This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5231-5240.1987
更新日期:1987-11-01 00:00:00
abstract::Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1538-1541.1980
更新日期:1980-09-01 00:00:00
abstract::An early step in crown gall tumor formation involves the attachment of Agrobacterium tumefaciens to host plant cells. A. tumefaciens C58::A205 (C58 attR) is a Tn3HoHo1 insertion mutant that was found to be avirulent on Bryophyllum daigremontiana and unable to attach to carrot suspension cells. The mutation mapped to a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.17.5372-5379.1997
更新日期:1997-09-01 00:00:00
abstract::The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.289-290.1978
更新日期:1978-07-01 00:00:00
abstract::Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5036-5042.1992
更新日期:1992-08-01 00:00:00
abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.603-607.1980
更新日期:1980-11-01 00:00:00
abstract::N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2902-2910.1996
更新日期:1996-05-01 00:00:00
abstract::In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00992-09
更新日期:2009-12-01 00:00:00
abstract::Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00235-10
更新日期:2010-10-01 00:00:00
abstract::The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2469-2482.2005
更新日期:2005-04-01 00:00:00
abstract::Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2711-2715.1988
更新日期:1988-06-01 00:00:00
abstract::We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.18.6046-6048.1993
更新日期:1993-09-01 00:00:00
abstract::Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3414-3421.1987
更新日期:1987-08-01 00:00:00
abstract::The exosporium of Bacillus anthracis is comprised of two distinct layers: a basal layer and a hair-like nap that covers the basal layer. The hair-like nap contains the glycoproteins BclA and, most likely, BclB. BclA and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains. Here, we i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05658-11
更新日期:2011-10-01 00:00:00
abstract::Plasmid Rms312, specifying resistance to tetracycline (Tc), chloramphenicol (Cm), streptomycin (Sm), sulfonamide (Su), and mercury chloride (Mer), deletes both Tc and Cm Sm Su Mer determinants at a high frequency in Salmonella typhimurium LT2. S. typhimurium mutants that were stable carriers of Rms312 were isolated by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.145-152.1980
更新日期:1980-04-01 00:00:00
abstract::t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01548-14
更新日期:2014-06-01 00:00:00
abstract::Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00261-07
更新日期:2007-06-01 00:00:00
abstract::The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01253-09
更新日期:2010-02-01 00:00:00
abstract::Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Se...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01804-08
更新日期:2009-06-01 00:00:00
abstract::The products of the bvgAS locus coordinately regulate expression of the Bordetella pertussis virulence regulon in response to environmental signals. Transcription of bvgAS-activated genes is nearly eliminated by several modulating conditions, including the presence of sulfate anion or nicotinic acid and growth at low ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.970-979.1992
更新日期:1992-02-01 00:00:00
abstract::Plasmids encoding the structural genes for the Rhodobacter capsulatus and Rhodobacter sphaeroides cytochrome (cyt) bc1 complexes were introduced into strains of R. capsulatus lacking the cyt bc1 complex, with and without cyt c2. The R. capsulatus merodiploids contained higher than wild-type levels of cyt bc1 complex, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6059-6068.1989
更新日期:1989-11-01 00:00:00