Abstract:
:In wild-type Escherichia coli the activity of the maltose transport system is dependent on a periplasmic maltose-binding protein. It has been possible, however, to isolate mutants in which transport activity is mediated by the membrane components of the system and is no longer dependent on the periplasmic binding protein. In this manuscript we show that in these binding protein-independent strains, p-nitrophenyl-alpha-maltoside is a potent inhibitor of maltose transport. In contrast, p-nitrophenyl-alpha-maltoside is only a weak inhibitor of maltose transport in wild-type bacteria. In addition, we show that p-nitrophenyl-alpha-maltoside is transported by the binding protein-independent strains but not by wild-type bacteria. We were able to detect transport of this compound because there is a cytoplasmic enzyme that cleaves p-nitrophenyl-alpha-maltoside. This enzyme has not previously been described. We show that although the synthesis of this enzyme is subject to the same regulation as the components of the maltose regulon, and is MalT dependent, it is not coded for by a known mal gene. We refer to this enzyme as alpha-maltosidase. These results strengthen our proposal that the membrane components of the maltose transport system comprise a recognition site for maltose and related substrates.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Reyes M,Treptow NA,Shuman HAdoi
10.1128/jb.165.3.918-922.1986subject
Has Abstractpub_date
1986-03-01 00:00:00pages
918-22issue
3eissn
0021-9193issn
1098-5530journal_volume
165pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.182.4.1035-1045.2000
更新日期:2000-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.176.3.656-664.1994
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.125.2.744-746.1976
更新日期:1976-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5803-5811.1989
更新日期:1989-11-01 00:00:00
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更新日期:2000-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1197-1204.1988
更新日期:1988-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1965-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1658-1665.1988
更新日期:1988-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.139-146.1974
更新日期:1974-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.970-974.1968
更新日期:1968-10-01 00:00:00
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更新日期:2003-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.316-322.1973
更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6649-6655.1989
更新日期:1989-12-01 00:00:00
abstract::Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5768-5775.1989
更新日期:1989-11-01 00:00:00